ID   PIRC2_MOUSE             Reviewed;         122 AA.
AC   V9GXK1;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   19-FEB-2014, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=Piercer of microtubule wall 2 protein;
GN   Name=PIERCE2; Synonyms=Ccpg1os {ECO:0000312|MGI:MGI:3648770};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN   [1] {ECO:0000312|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J;
RG   Ensembl;
RL   Submitted (DEC-2013) to UniProtKB.
RN   [3]
RP   DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Microtubule inner protein involved in the attachment of outer
CC       dynein arms (ODAs) to dynein-decorated doublet microtubules (DMTs) in
CC       cilia axoneme, which is required for motile cilia beating.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SUBUNIT: Interacts with CFAP53, ODAD1 and ODAD3; the interactions link
CC       the outer dynein arms docking complex (ODA-DC) to the internal
CC       microtubule inner proteins (MIP) in cilium axoneme.
CC       {ECO:0000250|UniProtKB:A0A3Q1LFK7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000250|UniProtKB:A0A3Q1LFK7}.
CC   -!- DISRUPTION PHENOTYPE: Mutants born at the expected frequency
CC       (PubMed:34715025). At 8 dpc, they have a reduced beat frequency of
CC       nodal cilia (PubMed:34715025). The motility of tracheal cilia in adult
CC       mice is also affected (PubMed:34715025). 25% of tracheal cilia axonemes
CC       have missing dynein arms (PubMed:34715025). Double PIERCE1 and PIERCE2
CC       mutants show high levels of embryonic and pre-weaning lethality. The
CC       few mice that survive birth display hydrocephalus and laterality
CC       abnormalities, dying by 20 days of age (PubMed:34715025).
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SIMILARITY: Belongs to the PIERCE2 family. {ECO:0000305}.
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DR   RefSeq; NP_001185718.1; NM_001198789.1.
DR   AlphaFoldDB; V9GXK1; -.
DR   SMR; V9GXK1; -.
DR   PRIDE; V9GXK1; -.
DR   ProteomicsDB; 308559; -.
DR   Antibodypedia; 73844; 5 antibodies from 5 providers.
DR   Ensembl; ENSMUST00000124565; ENSMUSP00000139187; ENSMUSG00000086158.
DR   GeneID; 546143; -.
DR   KEGG; mmu:546143; -.
DR   UCSC; uc009qqk.3; mouse.
DR   CTD; 145788; -.
DR   MGI; MGI:3648770; Ccpg1os.
DR   VEuPathDB; HostDB:ENSMUSG00000086158; -.
DR   eggNOG; ENOG502S7B5; Eukaryota.
DR   GeneTree; ENSGT00940000154745; -.
DR   HOGENOM; CLU_153970_0_0_1; -.
DR   OMA; MIMYKTS; -.
DR   OrthoDB; 1488437at2759; -.
DR   PhylomeDB; V9GXK1; -.
DR   BioGRID-ORCS; 546143; 3 hits in 66 CRISPR screens.
DR   ChiTaRS; Ccpg1os; mouse.
DR   Proteomes; UP000000589; Chromosome 9.
DR   RNAct; V9GXK1; protein.
DR   Bgee; ENSMUSG00000086158; Expressed in spermatid and 182 other tissues.
DR   GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR   GO; GO:0035082; P:axoneme assembly; IMP:UniProtKB.
DR   GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR   GO; GO:0007368; P:determination of left/right symmetry; IMP:UniProtKB.
DR   InterPro; IPR026507; UPF0691.
DR   PANTHER; PTHR20899; PTHR20899; 1.
DR   Pfam; PF14892; DUF4490; 1.
PE   3: Inferred from homology;
KW   Cell projection; Cytoplasm; Cytoskeleton; Reference proteome.
FT   CHAIN           1..122
FT                   /note="Piercer of microtubule wall 2 protein"
FT                   /id="PRO_0000455517"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          99..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..18
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   122 AA;  13714 MW;  EBA40B89A5347590 CRC64;
     MARETDCDLD KKTSLTSDAE MRPEPPALCV NPGNPVFSCM LDPKTLHTAT SLSKAQMIMY
     KTSASQYGAF SPRPFFFPCK FLPQEQAFTE HLKTTGFYQN NSLNVGPDRT RTIDSPNYQH
     TL