PIRL1_ARATH
ID PIRL1_ARATH Reviewed; 506 AA.
AC Q9FFJ3;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Plant intracellular Ras-group-related LRR protein 1;
GN Name=PIRL1; OrderedLocusNames=At5g05850; ORFNames=MJJ3.27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, MOTIF GVYW, AND TISSUE
RP SPECIFICITY.
RX PubMed=15809230; DOI=10.1093/pcp/pci097;
RA Forsthoefel N.R., Cutler K., Port M.D., Yamamoto T., Vernon D.M.;
RT "PIRLs: a novel class of plant intracellular leucine-rich repeat
RT proteins.";
RL Plant Cell Physiol. 46:913-922(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20697737; DOI=10.1007/s00425-010-1242-6;
RA Forsthoefel N.R., Dao T.P., Vernon D.M.;
RT "PIRL1 and PIRL9, encoding members of a novel plant-specific family of
RT leucine-rich repeat proteins, are essential for differentiation of
RT microspores into pollen.";
RL Planta 232:1101-1114(2010).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21140166; DOI=10.1007/s00425-010-1324-5;
RA Forsthoefel N.R., Vernon D.M.;
RT "Effect of sporophytic PIRL9 genotype on post-meiotic expression of the
RT Arabidopsis pirl1;pirl9 mutant pollen phenotype.";
RL Planta 233:423-431(2011).
CC -!- FUNCTION: Leucine-rich repeat protein that likely mediates protein
CC interactions, possibly in the context of signal transduction. PIRL1
CC acts redundantly with PIRL9 in the differentiation of microspores into
CC pollen. {ECO:0000269|PubMed:20697737, ECO:0000269|PubMed:21140166}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:15809230}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Pirl1 and pirl9 double
CC mutant is lethal due to a male-specific transmission failure leading to
CC a severe pollen malformation. {ECO:0000269|PubMed:20697737,
CC ECO:0000269|PubMed:21140166}.
CC -!- SIMILARITY: Belongs to the SHOC2 family. {ECO:0000305}.
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DR EMBL; AY849571; AAW57410.1; -; mRNA.
DR EMBL; AB005237; BAB09679.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90932.1; -; Genomic_DNA.
DR EMBL; BT015883; AAU95419.1; -; mRNA.
DR EMBL; BT020196; AAV59262.1; -; mRNA.
DR EMBL; AK228975; BAF00864.1; -; mRNA.
DR RefSeq; NP_196204.1; NM_120667.5.
DR AlphaFoldDB; Q9FFJ3; -.
DR SMR; Q9FFJ3; -.
DR STRING; 3702.AT5G05850.1; -.
DR iPTMnet; Q9FFJ3; -.
DR PaxDb; Q9FFJ3; -.
DR PRIDE; Q9FFJ3; -.
DR ProteomicsDB; 234962; -.
DR EnsemblPlants; AT5G05850.1; AT5G05850.1; AT5G05850.
DR GeneID; 830470; -.
DR Gramene; AT5G05850.1; AT5G05850.1; AT5G05850.
DR KEGG; ath:AT5G05850; -.
DR Araport; AT5G05850; -.
DR TAIR; locus:2166429; AT5G05850.
DR eggNOG; KOG0619; Eukaryota.
DR HOGENOM; CLU_021557_0_0_1; -.
DR InParanoid; Q9FFJ3; -.
DR OMA; ETHDAYE; -.
DR OrthoDB; 783662at2759; -.
DR PhylomeDB; Q9FFJ3; -.
DR PRO; PR:Q9FFJ3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFJ3; baseline and differential.
DR Genevisible; Q9FFJ3; AT.
DR GO; GO:0055046; P:microgametogenesis; IGI:UniProtKB.
DR GO; GO:0009555; P:pollen development; IGI:UniProtKB.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13855; LRR_8; 2.
DR SMART; SM00369; LRR_TYP; 9.
DR PROSITE; PS51450; LRR; 9.
PE 2: Evidence at transcript level;
KW Coiled coil; Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..506
FT /note="Plant intracellular Ras-group-related LRR protein 1"
FT /id="PRO_0000423601"
FT REPEAT 203..225
FT /note="LRR 1"
FT REPEAT 226..249
FT /note="LRR 2"
FT REPEAT 251..272
FT /note="LRR 3"
FT REPEAT 273..295
FT /note="LRR 4"
FT REPEAT 297..319
FT /note="LRR 5"
FT REPEAT 320..342
FT /note="LRR 6"
FT REPEAT 344..364
FT /note="LRR 7"
FT REPEAT 365..389
FT /note="LRR 8"
FT REPEAT 390..412
FT /note="LRR 9"
FT REPEAT 414..436
FT /note="LRR 10"
FT REGION 24..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 143..193
FT /evidence="ECO:0000255"
FT MOTIF 437..449
FT /note="GVYW"
SQ SEQUENCE 506 AA; 56578 MW; C5368FE28D1EE3F1 CRC64;
MATELNPKNF PVLSYVLDRL PSFTAKSSSS SDVEPPPSKS DPSSSSNHSI EIVTQMPHLA
HPDVLASMTN ATADVSQTRS VLRTLGPRPD HETVDRARAR LREIDASLSE SFEEIALSPN
DIDVAEKEQK RREAVEQEKI WYKSILKLNE LHESYEKLLK EAEERLVRIY ESAEKNAAAV
AEEEAAEVEV NEEVVSILQQ AAENPLDRVD LSGRKLKLLP EAFGKIQGLL VLNLYNNQLQ
AIPDSIAGLH NLLELDVSTN FLETLPDSIG LLSKLKILNV SCNKLTTLPD SICHCGSLVV
LDASYNNLTY LPTNIGFELV KLEKLLIHLN KIRSLPTSIG EMRSLRYLDA HFNELNGLPN
SFGLLTNLEY LNLSSNFSDL QDLPASFGDL ISLQELDLSN NQIHSLPDAF GTLVNLTKLN
LDQNPLVVPP DEVVKQGVDA VKMYMGKRWV SMLEEEEKMA NMKDEMDQTN TDWLTRTTSK
LKTYVTEVSE YLGSNPPRDP YLDQQL
