ID   PIRL9_ARATH             Reviewed;         499 AA.
AC   Q8VYG9; Q9C769;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 127.
DE   RecName: Full=Plant intracellular Ras-group-related LRR protein 9;
GN   Name=PIRL9; OrderedLocusNames=At3g11330; ORFNames=F11B9.22;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, MOTIF GVYW, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=15809230; DOI=10.1093/pcp/pci097;
RA   Forsthoefel N.R., Cutler K., Port M.D., Yamamoto T., Vernon D.M.;
RT   "PIRLs: a novel class of plant intracellular leucine-rich repeat
RT   proteins.";
RL   Plant Cell Physiol. 46:913-922(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20697737; DOI=10.1007/s00425-010-1242-6;
RA   Forsthoefel N.R., Dao T.P., Vernon D.M.;
RT   "PIRL1 and PIRL9, encoding members of a novel plant-specific family of
RT   leucine-rich repeat proteins, are essential for differentiation of
RT   microspores into pollen.";
RL   Planta 232:1101-1114(2010).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=21140166; DOI=10.1007/s00425-010-1324-5;
RA   Forsthoefel N.R., Vernon D.M.;
RT   "Effect of sporophytic PIRL9 genotype on post-meiotic expression of the
RT   Arabidopsis pirl1;pirl9 mutant pollen phenotype.";
RL   Planta 233:423-431(2011).
CC   -!- FUNCTION: Leucine-rich repeat protein that likely mediates protein
CC       interactions, possibly in the context of signal transduction. PIRL9
CC       acts redundantly with PIRL1 in the differentiation of microspores into
CC       pollen. {ECO:0000269|PubMed:20697737, ECO:0000269|PubMed:21140166}.
CC   -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:15809230}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype. Pirl1 and pirl9 double
CC       mutant is lethal due to a male-specific transmission failure leading to
CC       a severe pollen malformation. {ECO:0000269|PubMed:20697737,
CC       ECO:0000269|PubMed:21140166}.
CC   -!- SIMILARITY: Belongs to the SHOC2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG50968.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY849579; AAW57418.1; -; mRNA.
DR   EMBL; AC073395; AAG50968.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE75031.1; -; Genomic_DNA.
DR   EMBL; AY072078; AAL59901.1; -; mRNA.
DR   EMBL; AY122958; AAM67491.1; -; mRNA.
DR   RefSeq; NP_187741.2; NM_111967.4.
DR   AlphaFoldDB; Q8VYG9; -.
DR   SMR; Q8VYG9; -.
DR   STRING; 3702.AT3G11330.1; -.
DR   iPTMnet; Q8VYG9; -.
DR   PaxDb; Q8VYG9; -.
DR   PRIDE; Q8VYG9; -.
DR   ProteomicsDB; 235027; -.
DR   EnsemblPlants; AT3G11330.1; AT3G11330.1; AT3G11330.
DR   GeneID; 820306; -.
DR   Gramene; AT3G11330.1; AT3G11330.1; AT3G11330.
DR   KEGG; ath:AT3G11330; -.
DR   Araport; AT3G11330; -.
DR   TAIR; locus:2074728; AT3G11330.
DR   eggNOG; KOG0619; Eukaryota.
DR   HOGENOM; CLU_021557_0_0_1; -.
DR   InParanoid; Q8VYG9; -.
DR   OMA; AVKTYMG; -.
DR   OrthoDB; 783662at2759; -.
DR   PhylomeDB; Q8VYG9; -.
DR   PRO; PR:Q8VYG9; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q8VYG9; baseline and differential.
DR   Genevisible; Q8VYG9; AT.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0055046; P:microgametogenesis; IGI:UniProtKB.
DR   GO; GO:0009555; P:pollen development; IGI:UniProtKB.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   Pfam; PF13855; LRR_8; 3.
DR   SMART; SM00369; LRR_TYP; 9.
DR   PROSITE; PS51450; LRR; 8.
PE   2: Evidence at transcript level;
KW   Coiled coil; Leucine-rich repeat; Reference proteome; Repeat.
FT   CHAIN           1..499
FT                   /note="Plant intracellular Ras-group-related LRR protein 9"
FT                   /id="PRO_0000423609"
FT   REPEAT          197..219
FT                   /note="LRR 1"
FT   REPEAT          220..243
FT                   /note="LRR 2"
FT   REPEAT          245..266
FT                   /note="LRR 3"
FT   REPEAT          267..289
FT                   /note="LRR 4"
FT   REPEAT          291..312
FT                   /note="LRR 5"
FT   REPEAT          313..336
FT                   /note="LRR 6"
FT   REPEAT          338..359
FT                   /note="LRR 7"
FT   REPEAT          360..383
FT                   /note="LRR 8"
FT   REPEAT          384..406
FT                   /note="LRR 9"
FT   REPEAT          408..430
FT                   /note="LRR 10"
FT   REGION          25..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          138..187
FT                   /evidence="ECO:0000255"
FT   MOTIF           431..438
FT                   /note="GVYW; degenerate"
SQ   SEQUENCE   499 AA;  55503 MW;  35166D473D42714E CRC64;
     MAAEPNPKNF PVLSYVLARL PSFTAKSPSS SVPPFDIEQP PPSSSSSSIE IVTQMPHLTQ
     PDVLASMTSA ISDVAETRSI LRTLGPRPDH ESVDKARAKL SEIESFLSES FEDIALTDAA
     AKDEKRRQEM DQEKTWCESI LKLDEVHASY EKLLKEAEER LVRIYESAEK NAAEDEENVA
     AVEVNEEVVG ILQHASANPV DRVDLSGRKL RLLPEAFGRI QGLLVLNLSN NKLESIPDSI
     AGLHSLVELD VSTNSLETLP DSIGLLSKLK ILNVSTNKLT SLPDSICRCG SLVILDVSFN
     RLTYLPTNIG PELVNLEKLL VQYNKIRSFP TSIGEMRSLK HLDAHFNELN GLPDSFVLLT
     NLEYLNLSSN FSDLKDLPFS FGELISLQEL DLSNNQIHAL PDTFGTLDSL TKLNVDQNPL
     VVPPEEVVKE GVEAVKTYMG QRRISMLEEE EKKKMEEEME QANAGWLTRT TSKLKTYVAD
     VSEYLGSNSP RDPYLERQL