ID   PIRT_HUMAN              Reviewed;         137 AA.
AC   P0C851; B7Z648;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Phosphoinositide-interacting protein;
GN   Name=PIRT;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Fetal brain, and Hippocampus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16625196; DOI=10.1038/nature04689;
RA   Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA   Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA   Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA   Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA   DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA   Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA   Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA   LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA   Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA   Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA   Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA   Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA   Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT   "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT   human lineage.";
RL   Nature 440:1045-1049(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulatory subunit of TRPV1, a molecular sensor of noxious
CC       heat and capsaicin. Positively regulates TRPV1 channel activity via
CC       phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various
CC       phosphoinositide, including phosphatidylinositol 4,5-bisphosphate
CC       (PIP2), but not phosphatidylinositol (PI) (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with TRPV1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-3 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; AK299808; BAH13134.1; -; mRNA.
DR   EMBL; DC332889; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AC015908; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471108; EAW89989.1; -; Genomic_DNA.
DR   CCDS; CCDS45614.1; -.
DR   RefSeq; NP_001094857.1; NM_001101387.1.
DR   AlphaFoldDB; P0C851; -.
DR   BioGRID; 569345; 1.
DR   STRING; 9606.ENSP00000462046; -.
DR   TCDB; 8.A.64.1.1; the phosphoinositide-interacting protein (pirt) family.
DR   iPTMnet; P0C851; -.
DR   PhosphoSitePlus; P0C851; -.
DR   BioMuta; PIRT; -.
DR   DMDM; 308153473; -.
DR   jPOST; P0C851; -.
DR   MassIVE; P0C851; -.
DR   PaxDb; P0C851; -.
DR   PeptideAtlas; P0C851; -.
DR   PRIDE; P0C851; -.
DR   ProteomicsDB; 52394; -.
DR   Antibodypedia; 47967; 33 antibodies from 8 providers.
DR   DNASU; 644139; -.
DR   Ensembl; ENST00000580256.3; ENSP00000462046.1; ENSG00000233670.7.
DR   GeneID; 644139; -.
DR   KEGG; hsa:644139; -.
DR   MANE-Select; ENST00000580256.3; ENSP00000462046.1; NM_001101387.2; NP_001094857.1.
DR   UCSC; uc010col.4; human.
DR   CTD; 644139; -.
DR   GeneCards; PIRT; -.
DR   HGNC; HGNC:37239; PIRT.
DR   HPA; ENSG00000233670; Tissue enriched (brain).
DR   MIM; 612068; gene.
DR   neXtProt; NX_P0C851; -.
DR   OpenTargets; ENSG00000233670; -.
DR   VEuPathDB; HostDB:ENSG00000233670; -.
DR   eggNOG; ENOG502S54V; Eukaryota.
DR   GeneTree; ENSGT00940000154322; -.
DR   HOGENOM; CLU_1906059_0_0_1; -.
DR   InParanoid; P0C851; -.
DR   OMA; WIPIIRK; -.
DR   OrthoDB; 1610805at2759; -.
DR   PhylomeDB; P0C851; -.
DR   TreeFam; TF328608; -.
DR   PathwayCommons; P0C851; -.
DR   BioGRID-ORCS; 644139; 16 hits in 1063 CRISPR screens.
DR   GenomeRNAi; 644139; -.
DR   Pharos; P0C851; Tdark.
DR   PRO; PR:P0C851; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; P0C851; protein.
DR   Bgee; ENSG00000233670; Expressed in dorsal root ganglion and 77 other tissues.
DR   Genevisible; P0C851; HS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:1902936; F:phosphatidylinositol bisphosphate binding; IBA:GO_Central.
DR   GO; GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; IEA:Ensembl.
DR   GO; GO:0044325; F:transmembrane transporter binding; IBA:GO_Central.
DR   GO; GO:0048266; P:behavioral response to pain; IEA:Ensembl.
DR   GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR   GO; GO:2001259; P:positive regulation of cation channel activity; IBA:GO_Central.
DR   GO; GO:0009408; P:response to heat; IEA:Ensembl.
DR   InterPro; IPR028068; PIRT.
DR   Pfam; PF15099; PIRT; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..137
FT                   /note="Phosphoinositide-interacting protein"
FT                   /id="PRO_0000347060"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   137 AA;  15334 MW;  588C1AA13A5BDD05 CRC64;
     MTMETLPKVL EVDEKSPEAK DLLPSQTASS LCISSRSESV WTTTPRSNWE IYRKPIVIMS
     VGGAILLFGV VITCLAYTLK LSDKSLSILK MVGPGFLSLG LMMLVCGLVW VPIIKKKQKH
     RQKSNFLRSL KSFFLTR