ID   PIR_ARATH               Reviewed;        1282 AA.
AC   Q5S2C3; Q3E9F2; Q6DLU3;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Protein PIR;
DE   AltName: Full=PIR of plants;
DE   AltName: Full=Protein KLUNKER;
DE            Short=AtSRA1;
DE   AltName: Full=Protein PIROGI;
GN   Name=PIR; Synonyms=KLK, PIRP; OrderedLocusNames=At5g18410;
GN   ORFNames=F20L16.130;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX   PubMed=15516496; DOI=10.1104/pp.104.053173;
RA   Li Y., Sorefan K., Hemmann G., Bevan M.W.;
RT   "Arabidopsis NAP and PIR regulate actin-based cell morphogenesis and
RT   multiple developmental processes.";
RL   Plant Physiol. 136:3616-3627(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH NAP1 AND RPO2, AND
RP   TISSUE SPECIFICITY.
RX   PubMed=15294869; DOI=10.1242/dev.01307;
RA   Basu D., El-Din El-Assal S., Le J., Mallery E.L., Szymanski D.B.;
RT   "Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex
RT   subunit SRA1 during leaf epidermal development.";
RL   Development 131:4345-4355(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION (ISOFORM 2), AND
RP   TISSUE SPECIFICITY.
RX   PubMed=15316111; DOI=10.1105/tpc.104.023739;
RA   Brembu T., Winge P., Seem M., Bones A.M.;
RT   "NAPP and PIRP encode subunits of a putative wave regulatory protein
RT   complex involved in plant cell morphogenesis.";
RL   Plant Cell 16:2335-2349(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [6]
RP   TISSUE SPECIFICITY, AND INTERACTION WITH NAP1.
RX   PubMed=15296760; DOI=10.1016/j.cub.2004.06.062;
RA   El-Din El-Assal S., Le J., Basu D., Mallery E.L., Szymanski D.B.;
RT   "Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates
RT   ARP2/3.";
RL   Curr. Biol. 14:1405-1409(2004).
CC   -!- FUNCTION: Involved in regulation of actin and microtubule organization.
CC       Part of a WAVE complex that activates the ARP2/3 complex. Interacts
CC       with the active form of RHO-family GTPases.
CC   -!- SUBUNIT: Binds NAP1 and ROP2, but not ROP8.
CC   -!- INTERACTION:
CC       Q5S2C3; Q5S2C4: NAP1; NbExp=5; IntAct=EBI-1547858, EBI-1547830;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q5S2C3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q5S2C3-2; Sequence=VSP_041592;
CC   -!- TISSUE SPECIFICITY: Expressed in roots, root hairs, hypocotyls,
CC       cotyledons, stems, leaves, trichomes and flowers.
CC       {ECO:0000269|PubMed:15294869, ECO:0000269|PubMed:15296760,
CC       ECO:0000269|PubMed:15316111, ECO:0000269|PubMed:15516496}.
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DR   EMBL; AY787212; AAV64873.1; -; mRNA.
DR   EMBL; AY662957; AAT71307.1; -; mRNA.
DR   EMBL; AY496701; AAS78644.1; -; mRNA.
DR   EMBL; AC051626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED92556.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED92557.1; -; Genomic_DNA.
DR   EMBL; BK004073; DAA04564.1; -; Genomic_DNA.
DR   RefSeq; NP_197342.3; NM_121846.4. [Q5S2C3-2]
DR   RefSeq; NP_974801.2; NM_203072.3. [Q5S2C3-1]
DR   AlphaFoldDB; Q5S2C3; -.
DR   SMR; Q5S2C3; -.
DR   BioGRID; 17235; 8.
DR   DIP; DIP-29824N; -.
DR   IntAct; Q5S2C3; 7.
DR   STRING; 3702.AT5G18410.1; -.
DR   PaxDb; Q5S2C3; -.
DR   PRIDE; Q5S2C3; -.
DR   ProteomicsDB; 234762; -. [Q5S2C3-1]
DR   EnsemblPlants; AT5G18410.1; AT5G18410.1; AT5G18410. [Q5S2C3-2]
DR   EnsemblPlants; AT5G18410.2; AT5G18410.2; AT5G18410. [Q5S2C3-1]
DR   GeneID; 831959; -.
DR   Gramene; AT5G18410.1; AT5G18410.1; AT5G18410. [Q5S2C3-2]
DR   Gramene; AT5G18410.2; AT5G18410.2; AT5G18410. [Q5S2C3-1]
DR   KEGG; ath:AT5G18410; -.
DR   Araport; AT5G18410; -.
DR   TAIR; locus:2146193; AT5G18410.
DR   eggNOG; KOG3534; Eukaryota.
DR   InParanoid; Q5S2C3; -.
DR   OMA; MPKVCKL; -.
DR   OrthoDB; 108507at2759; -.
DR   PRO; PR:Q5S2C3; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q5S2C3; baseline and differential.
DR   Genevisible; Q5S2C3; AT.
DR   GO; GO:0031209; C:SCAR complex; IBA:GO_Central.
DR   GO; GO:0000340; F:RNA 7-methylguanosine cap binding; IBA:GO_Central.
DR   GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IMP:TAIR.
DR   GO; GO:0045010; P:actin nucleation; TAS:TAIR.
DR   GO; GO:0000902; P:cell morphogenesis; IBA:GO_Central.
DR   GO; GO:0030833; P:regulation of actin filament polymerization; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR   GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR   InterPro; IPR009828; CYRIA/CYRIB_Rac1-bd.
DR   InterPro; IPR008081; Cytoplasmic_FMR1-int.
DR   PANTHER; PTHR12195; PTHR12195; 1.
DR   Pfam; PF07159; DUF1394; 1.
DR   Pfam; PF05994; FragX_IP; 1.
DR   PIRSF; PIRSF008153; FMR1_interacting; 1.
DR   PRINTS; PR01698; CYTOFMRPINTP.
PE   1: Evidence at protein level;
KW   Alternative splicing; Reference proteome.
FT   CHAIN           1..1282
FT                   /note="Protein PIR"
FT                   /id="PRO_0000076292"
FT   VAR_SEQ         1022..1023
FT                   /note="RE -> VSP (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_041592"
SQ   SEQUENCE   1282 AA;  145488 MW;  3755F9E5D025D55E CRC64;
     MAVPVEEAIA ALSTFSLEDE QPEVQGPAVM VSAERAATDS PIEYSDVAAY RLSLSEDTKA
     LNQLNTLIQE GKEMASILYT YRSCVKALPQ LPESMKHSQA DLYLETYQVL DLEMSRLREI
     QRWQSSASAK LAADMQRFSR PERRINGPTV THLWSMLKLL DVLVQLDHLK NAKASIPNDF
     SWYKRTFTQV SAQWQDTDTM REELDDLQIF LSTRWAILLN LHVEMFRVNN VEDILQVLIV
     FIVESLELDF ALLFPERYIL LRVLPVLVVL ATPSEKDTEA LYKRVKLNRL INIFKNDPVI
     PAFPDLHLSP AAILKELSVY FQKFSSQTRL LTLPAPHELP PREALEYQRH YLIVNHIGAL
     RAEHDDFTIR FASSMNQLLL LKSNDGAYTE WCREVKGNMY DMVVEGFQLL SRWTARIWEQ
     CAWKFSRPCR DAGETPEASG SYSDYEKVVR FNYTAEERKA LVELVGYIKS VGSMLQRCDT
     LVADALWETI HAEVQDFVQN TLATMLRTTF RKKKDLSRIL SDMRTLSADW MANTRPEHEM
     PSSQHGNDES RGNFFYPRPV APTAAQVHCL QFLIYEVVSG GNLRRPGGFF GNNGSEIPVN
     DLKQLETFFY KLSFFLHILD YSASIGILTD LGFLWFREFY LESSRVIQFP IECSLPWMLI
     DYILEAQNSG LLESVLLPFD IYNDSAQQAL VVLRQRFLYD EIEAEVDHGF DIFVSRLSES
     IFTYYKSWSA SELLDPSFLF ALDNGEKFSI QPVRFTALFK MTKVKILGRT INLRSLIAQR
     MNRIFRENLE FLFDRFESQD LCAVVELEKL IDILKHSHEL LSQDLSIDPF SLMLNEMQEN
     ISLVSFSSRL ATQIWSEMQS DFLPNFILCN TTQRFVRSSK VPPTQKPSVP SAKPSFYCGT
     QDLNAAHQSF ARLHSGFFGI PHLFSIVKLL GSRSLPWLIR ALLDHISNKI TTLEPMISGL
     QEALPKSIGL LSFDGGVTGC MKLIREQLNW GTKSELKSEV LRGIKEIGSV IYTMGLLDIV
     LREVDTKRFM QTAPWLGLIP GAEGQIVNAQ DGESPLVNLL KSATSAVVSS PGCLNPAAFY
     TMSKQAEAAD LLYKANMNGG SVLEYTLAFT SASLDKYCSK WSAPPKTGFV DITTSKDFYR
     IYGGLQIGYL EEITAPQSAQ HEVLGDSIAW GGCTIIYLLG QQLHFELFDF SYQVLNVSEV
     ETVSASHTHR NPQIHQGWEG LLEGMKKARR LNNHVFSMLK ARCPLEDKTA CAIKQSGAPL
     PRVRFENTVS AFETLPQKGT VG