PISP_NASVI
ID PISP_NASVI Reviewed; 80 AA.
AC A0A7M6UNN1;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 07-APR-2021, sequence version 1.
DT 03-AUG-2022, entry version 5.
DE RecName: Full=Small pacifastin protease inhibitor {ECO:0000303|PubMed:28283430};
DE Short=NvSPPI {ECO:0000303|PubMed:28283430};
DE Short=PPI {ECO:0000303|PubMed:28283430};
DE Flags: Precursor;
OS Nasonia vitripennis (Parasitic wasp).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Parasitoida; Chalcidoidea;
OC Pteromalidae; Pteromalinae; Nasonia.
OX NCBI_TaxID=7425;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AsymCX;
RX PubMed=20075255; DOI=10.1126/science.1178028;
RG Nasonia Genome Working Group;
RA Werren J.H., Richards S., Desjardins C.A., Niehuis O., Gadau J.,
RA Colbourne J.K., Werren J.H., Richards S., Desjardins C.A., Niehuis O.,
RA Gadau J., Colbourne J.K., Beukeboom L.W., Desplan C., Elsik C.G.,
RA Grimmelikhuijzen C.J., Kitts P., Lynch J.A., Murphy T., Oliveira D.C.,
RA Smith C.D., van de Zande L., Worley K.C., Zdobnov E.M., Aerts M.,
RA Albert S., Anaya V.H., Anzola J.M., Barchuk A.R., Behura S.K., Bera A.N.,
RA Berenbaum M.R., Bertossa R.C., Bitondi M.M., Bordenstein S.R., Bork P.,
RA Bornberg-Bauer E., Brunain M., Cazzamali G., Chaboub L., Chacko J.,
RA Chavez D., Childers C.P., Choi J.H., Clark M.E., Claudianos C.,
RA Clinton R.A., Cree A.G., Cristino A.S., Dang P.M., Darby A.C.,
RA de Graaf D.C., Devreese B., Dinh H.H., Edwards R., Elango N., Elhaik E.,
RA Ermolaeva O., Evans J.D., Foret S., Fowler G.R., Gerlach D., Gibson J.D.,
RA Gilbert D.G., Graur D., Grunder S., Hagen D.E., Han Y., Hauser F.,
RA Hultmark D., Hunter H.C. IV, Hurst G.D., Jhangian S.N., Jiang H.,
RA Johnson R.M., Jones A.K., Junier T., Kadowaki T., Kamping A., Kapustin Y.,
RA Kechavarzi B., Kim J., Kim J., Kiryutin B., Koevoets T., Kovar C.L.,
RA Kriventseva E.V., Kucharski R., Lee H., Lee S.L., Lees K., Lewis L.R.,
RA Loehlin D.W., Logsdon J.M. Jr., Lopez J.A., Lozado R.J., Maglott D.,
RA Maleszka R., Mayampurath A., Mazur D.J., McClure M.A., Moore A.D.,
RA Morgan M.B., Muller J., Munoz-Torres M.C., Muzny D.M., Nazareth L.V.,
RA Neupert S., Nguyen N.B., Nunes F.M., Oakeshott J.G., Okwuonu G.O.,
RA Pannebakker B.A., Pejaver V.R., Peng Z., Pratt S.C., Predel R., Pu L.L.,
RA Ranson H., Raychoudhury R., Rechtsteiner A., Reese J.T., Reid J.G.,
RA Riddle M., Robertson H.M., Romero-Severson J., Rosenberg M., Sackton T.B.,
RA Sattelle D.B., Schluns H., Schmitt T., Schneider M., Schuler A.,
RA Schurko A.M., Shuker D.M., Simoes Z.L., Sinha S., Smith Z., Solovyev V.,
RA Souvorov A., Springauf A., Stafflinger E., Stage D.E., Stanke M.,
RA Tanaka Y., Telschow A., Trent C., Vattathil S., Verhulst E.C.,
RA Viljakainen L., Wanner K.W., Waterhouse R.M., Whitfield J.B., Wilkes T.E.,
RA Williamson M., Willis J.H., Wolschin F., Wyder S., Yamada T., Yi S.V.,
RA Zecher C.N., Zhang L., Gibbs R.A.;
RT "Functional and evolutionary insights from the genomes of three parasitoid
RT Nasonia species.";
RL Science 327:343-348(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND RECOMBINANT EXPRESSION.
RX PubMed=28283430; DOI=10.1016/j.toxicon.2017.03.005;
RA Qian C., Liang D., Liu Y., Wang P., Kausar S., Wei G., Zhu B., Wang L.,
RA Liu C.;
RT "Identification of a small pacifastin protease inhibitor from Nasonia
RT vitripennis venom that inhibits humoral immunity of host (Musca
RT domestica).";
RL Toxicon 131:54-62(2017).
CC -!- FUNCTION: Parasitic wasp protein that may interfere with the host
CC immune response. The recombinant protein inhibits trypsin activity and
CC prophenoloxidase (PPO) activation, an enzyme essential for both
CC clotting and insect innate immune responses. It does not inhibit
CC activity of chymotrypsin and protease K, and has no effect on
CC phenoloxidase (PO) activity. {ECO:0000269|PubMed:28283430}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:28283430}.
CC -!- TISSUE SPECIFICITY: Expressed in the venom apparatus. Low transcript
CC levels are also detected in other tissues.
CC {ECO:0000269|PubMed:28283430}.
CC -!- DEVELOPMENTAL STAGE: Transcripts are not detected at 0 and 1st day post
CC eclosion (dpe), weakly expressed at 2nd and 3rd dpe, reach a peak on
CC the 4th dpe, and then decline gradually until 7th dpe.
CC {ECO:0000269|PubMed:28283430}.
CC -!- SIMILARITY: Belongs to the protease inhibitor I19 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; NP_001155083.1; NM_001161611.1.
DR SMR; A0A7M6UNN1; -.
DR GeneID; 100123852; -.
DR KEGG; nvi:100123852; -.
DR Proteomes; UP000002358; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR008037; Pacifastin_dom.
DR InterPro; IPR036201; Pacifastin_dom_sf.
DR Pfam; PF05375; Pacifastin_I; 1.
DR SUPFAM; SSF57283; SSF57283; 1.
DR PROSITE; PS51446; PACIFASTIN; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..47
FT /evidence="ECO:0000305"
FT /id="PRO_0000454319"
FT CHAIN 48..80
FT /note="Small pacifastin protease inhibitor"
FT /evidence="ECO:0000305"
FT /id="PRO_5029545642"
FT DOMAIN 45..80
FT /note="Pacifastin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00776"
FT DISULFID 48..63
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00776"
FT DISULFID 58..77
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00776"
FT DISULFID 61..72
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00776"
SQ SEQUENCE 80 AA; 8615 MW; 8A0AD8246F447C0A CRC64;
MSKVLKVGLL LLLVAVAASA YAVAEENGAP KENKQLPQID DYGVTNKCPA NQPFKWNCNY
CTCGPEGKDA SCTRMACPQH
