PIST_ARATH
ID PIST_ARATH Reviewed; 208 AA.
AC P48007; Q9SQ07; Q9SQ08; Q9SQ09; Q9SQ10; Q9SQ11; Q9SQ12; Q9SQ13;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Floral homeotic protein PISTILLATA;
DE AltName: Full=Transcription factor PI;
GN Name=PI; OrderedLocusNames=At5g20240; ORFNames=F5O24.130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=7958839; DOI=10.1101/gad.8.13.1548;
RA Goto K., Meyerowitz E.M.;
RT "Function and regulation of the Arabidopsis floral homeotic gene
RT PISTILLATA.";
RL Genes Dev. 8:1548-1560(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10769227; DOI=10.1242/dev.127.10.2021;
RA Honma T., Goto K.;
RT "The Arabidopsis floral homeotic gene PISTILLATA is regulated by discrete
RT cis-elements responsive to induction and maintenance signals.";
RL Development 127:2021-2030(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ALA-29; PRO-38; ASP-51;
RP MET-73; PRO-75; ASP-89; GLY-112; GLY-125; ILE-140; VAL-142; ILE-160 AND
RP THR-203.
RC STRAIN=cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Bu-2, cv. Chi-1,
RC cv. Co-1, cv. Columbia, cv. Corsacalla-1, cv. Cvi-0, cv. Gr-3, cv. Jl-1,
RC cv. Kas-1, cv. Kent, cv. Landsberg erecta, cv. Li-3, cv. Li-8, and
RC cv. Lisse;
RX PubMed=9927474; DOI=10.1093/genetics/151.2.839;
RA Purugganan M.D., Suddith J.I.;
RT "Molecular population genetics of floral homeotic loci: departures from the
RT equilibrium-neutral model at the APETALA3 and PISTILLATA genes of
RT Arabidopsis thaliana.";
RL Genetics 151:839-848(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP FUNCTION.
RX PubMed=8565821; DOI=10.1242/dev.122.1.11;
RA Krizek B.A., Meyerowitz E.M.;
RT "The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to
RT provide the B class organ identity function.";
RL Development 122:11-22(1996).
RN [7]
RP FUNCTION IN NAP EXPRESSION.
RX PubMed=9489703; DOI=10.1016/s0092-8674(00)80902-2;
RA Sablowski R.W.M., Meyerowitz E.M.;
RT "A homolog of NO APICAL MERISTEM is an immediate target of the floral
RT homeotic genes APETALA3/PISTILLATA.";
RL Cell 92:93-103(1998).
RN [8]
RP CHARACTERIZATION.
RX PubMed=8643482; DOI=10.1073/pnas.93.10.4793;
RA Riechmann J.L., Krizek B.A., Meyerowitz E.M.;
RT "Dimerization specificity of Arabidopsis MADS domain homeotic proteins
RT APETALA1, APETALA3, PISTILLATA, and AGAMOUS.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:4793-4798(1996).
RN [9]
RP INDUCTION.
RX PubMed=11283333; DOI=10.2307/3871337;
RA Ng M., Yanofsky M.F.;
RT "Activation of the Arabidopsis B class homeotic genes by APETALA1.";
RL Plant Cell 13:739-753(2001).
RN [10]
RP CHARACTERIZATION.
RX PubMed=11206550; DOI=10.1038/35054083;
RA Honma T., Goto K.;
RT "Complexes of MADS-box proteins are sufficient to convert leaves into
RT floral organs.";
RL Nature 409:525-529(2001).
RN [11]
RP FUNCTION.
RX PubMed=18417639; DOI=10.1104/pp.107.115634;
RA Mara C.D., Irish V.F.;
RT "Two GATA transcription factors are downstream effectors of floral homeotic
RT gene action in Arabidopsis.";
RL Plant Physiol. 147:707-718(2008).
RN [12]
RP INDUCTION BY EMF1 AND EMF2.
RX PubMed=19783648; DOI=10.1104/pp.109.143495;
RA Kim S.Y., Zhu T., Sung Z.R.;
RT "Epigenetic regulation of gene programs by EMF1 and EMF2 in Arabidopsis.";
RL Plant Physiol. 152:516-528(2010).
CC -!- FUNCTION: Probable transcription factor involved in the genetic control
CC of flower development. Is required for normal development of petals and
CC stamens in the wild-type flower. Forms a heterodimer with APETALA3 that
CC is required for autoregulation of both AP3 and PI genes. AP3/PI
CC heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary
CC complex that could be responsible for the regulation of the genes
CC involved in the flower development. AP3/PI heterodimer activates the
CC expression of NAP. AP3/PI prevents GATA22/GNL and GATA21/GNC expression
CC (PubMed:18417639). {ECO:0000269|PubMed:18417639,
CC ECO:0000269|PubMed:8565821, ECO:0000269|PubMed:9489703}.
CC -!- SUBUNIT: Forms a heterodimer with APETALA3, capable of binding to CArG-
CC box sequences. AP3/PI heterodimer binds AP1 or SEP3 to form a ternary
CC complex.
CC -!- INTERACTION:
CC P48007; O22456: SEP3; NbExp=3; IntAct=EBI-1395334, EBI-592020;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- INDUCTION: Positively regulated by the meristem identity proteins
CC APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing
CC mediated by polycomb group (PcG) protein complex containing EMF1 and
CC EMF2. {ECO:0000269|PubMed:11283333, ECO:0000269|PubMed:19783648}.
CC -!- MISCELLANEOUS: Mutations in PI cause transformation of petals into
CC sepals and stamina into carpels.
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DR EMBL; D30807; BAA06465.1; -; mRNA.
DR EMBL; AB035137; BAA87000.1; -; Genomic_DNA.
DR EMBL; AF115815; AAD51984.1; -; Genomic_DNA.
DR EMBL; AF115816; AAD51985.1; -; Genomic_DNA.
DR EMBL; AF115817; AAD51986.1; -; Genomic_DNA.
DR EMBL; AF115818; AAD51987.1; -; Genomic_DNA.
DR EMBL; AF115819; AAD51988.1; -; Genomic_DNA.
DR EMBL; AF115820; AAD51989.1; -; Genomic_DNA.
DR EMBL; AF115821; AAD51990.1; -; Genomic_DNA.
DR EMBL; AF115822; AAD51991.1; -; Genomic_DNA.
DR EMBL; AF115823; AAD51992.1; -; Genomic_DNA.
DR EMBL; AF115824; AAD51993.1; -; Genomic_DNA.
DR EMBL; AF115825; AAD51994.1; -; Genomic_DNA.
DR EMBL; AF115826; AAD51995.1; -; Genomic_DNA.
DR EMBL; AF115827; AAD51996.1; -; Genomic_DNA.
DR EMBL; AF115828; AAD51997.1; -; Genomic_DNA.
DR EMBL; AF115829; AAD51998.1; -; Genomic_DNA.
DR EMBL; AF115830; AAD51999.1; -; Genomic_DNA.
DR EMBL; AF296825; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92817.1; -; Genomic_DNA.
DR PIR; A53839; A53839.
DR RefSeq; NP_197524.1; NM_122031.4.
DR AlphaFoldDB; P48007; -.
DR SMR; P48007; -.
DR BioGRID; 17422; 21.
DR IntAct; P48007; 11.
DR STRING; 3702.AT5G20240.1; -.
DR PaxDb; P48007; -.
DR PRIDE; P48007; -.
DR ProteomicsDB; 236740; -.
DR EnsemblPlants; AT5G20240.1; AT5G20240.1; AT5G20240.
DR GeneID; 832146; -.
DR Gramene; AT5G20240.1; AT5G20240.1; AT5G20240.
DR KEGG; ath:AT5G20240; -.
DR Araport; AT5G20240; -.
DR TAIR; locus:2149264; AT5G20240.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_4_1; -.
DR InParanoid; P48007; -.
DR OMA; LVHMKGE; -.
DR OrthoDB; 1276495at2759; -.
DR PhylomeDB; P48007; -.
DR PRO; PR:P48007; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P48007; baseline and differential.
DR Genevisible; P48007; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010093; P:specification of floral organ identity; IMP:CACAO.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Developmental protein; Differentiation;
KW DNA-binding; Flowering; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..208
FT /note="Floral homeotic protein PISTILLATA"
FT /id="PRO_0000199482"
FT DOMAIN 3..57
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 84..170
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT COILED 75..117
FT /evidence="ECO:0000255"
FT VARIANT 29
FT /note="V -> A (in strain: cv. Cvi-0)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 38
FT /note="L -> P (in strain: cv. Co-1)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 51
FT /note="N -> D (in strain: cv. Lisse)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 73
FT /note="K -> M (in strain: cv. Co-1)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 75
FT /note="S -> P (in strain: cv. Kent)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 89
FT /note="N -> D (in strain: cv. Gr-3)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 112
FT /note="D -> G (in strain: cv. Lisse)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 125
FT /note="E -> G (in strain: cv. Gr-3)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 140
FT /note="M -> I (in strain: cv. Corsacalla-1)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 142
FT /note="I -> V (in strain: cv. Kent)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 160
FT /note="L -> I (in strain: cv. Kent)"
FT /evidence="ECO:0000269|PubMed:9927474"
FT VARIANT 203
FT /note="M -> T (in strain: cv. Cvi-0)"
FT /evidence="ECO:0000269|PubMed:9927474"
SQ SEQUENCE 208 AA; 24047 MW; 49728735AC883CCA CRC64;
MGRGKIEIKR IENANNRVVT FSKRRNGLVK KAKEITVLCD AKVALIIFAS NGKMIDYCCP
SMDLGAMLDQ YQKLSGKKLW DAKHENLSNE IDRIKKENDS LQLELRHLKG EDIQSLNLKN
LMAVEHAIEH GLDKVRDHQM EILISKRRNE KMMAEEQRQL TFQLQQQEMA IASNARGMMM
RDHDGQFGYR VQPIQPNLQE KIMSLVID
