PIT1_CANLF
ID PIT1_CANLF Reviewed; 291 AA.
AC Q9TTI7;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Pituitary-specific positive transcription factor 1;
DE Short=PIT-1;
DE AltName: Full=Growth hormone factor 1;
DE Short=GHF-1;
GN Name=POU1F1; Synonyms=GHF1, PIT1;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT LEU-81.
RX PubMed=10602989; DOI=10.1007/s003350010006;
RA Lantinga-van Leeuwen I.S., Mol J.A., Kooistra H.S., Rijnberk A., Breen M.,
RA Renier C., van Oost B.A.;
RT "Cloning of the canine gene encoding transcription factor Pit-1 and its
RT exclusion as candidate gene in a canine model of pituitary dwarfism.";
RL Mamm. Genome 11:31-36(2000).
CC -!- FUNCTION: Transcription factor involved in the specification of the
CC lactotrope, somatotrope, and thyrotrope phenotypes in the developing
CC anterior pituitary. Activates growth hormone and prolactin genes.
CC Specifically binds to the consensus sequence 5'-TAAAT-3'.
CC {ECO:0000250|UniProtKB:P28069}.
CC -!- SUBUNIT: Interacts with PITX1. Interacts with LHX3. Interacts with
CC ELK1. {ECO:0000250|UniProtKB:P28069}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28069}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:P28069}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-1
CC subfamily. {ECO:0000305}.
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DR EMBL; AF035585; AAF21643.1; -; mRNA.
DR RefSeq; NP_001006950.1; NM_001006949.1.
DR AlphaFoldDB; Q9TTI7; -.
DR SMR; Q9TTI7; -.
DR STRING; 9615.ENSCAFP00000011512; -.
DR Ensembl; ENSCAFT00030024206; ENSCAFP00030021110; ENSCAFG00030013046.
DR Ensembl; ENSCAFT00040040973; ENSCAFP00040035724; ENSCAFG00040022037.
DR Ensembl; ENSCAFT00845045738; ENSCAFP00845035896; ENSCAFG00845025896.
DR GeneID; 403753; -.
DR KEGG; cfa:403753; -.
DR CTD; 5449; -.
DR VEuPathDB; HostDB:ENSCAFG00845025896; -.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000158913; -.
DR InParanoid; Q9TTI7; -.
DR OrthoDB; 797288at2759; -.
DR Proteomes; UP000002254; Chromosome 31.
DR Bgee; ENSCAFG00000007781; Expressed in pituitary gland and 13 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:Ensembl.
DR GO; GO:0030183; P:B cell differentiation; IEA:Ensembl.
DR GO; GO:0001708; P:cell fate specification; IEA:Ensembl.
DR GO; GO:0008283; P:cell population proliferation; IEA:Ensembl.
DR GO; GO:0008340; P:determination of adult lifespan; IEA:Ensembl.
DR GO; GO:0032959; P:inositol trisphosphate biosynthetic process; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0032962; P:positive regulation of inositol trisphosphate biosynthetic process; IEA:Ensembl.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0043567; P:regulation of insulin-like growth factor receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060133; P:somatotropin secreting cell development; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR015586; Pit_1.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR PANTHER; PTHR11636:SF84; PTHR11636:SF84; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..291
FT /note="Pituitary-specific positive transcription factor 1"
FT /id="PRO_0000100696"
FT DOMAIN 124..198
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 214..273
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT MOTIF 5..13
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:P28069"
FT VARIANT 81
FT /note="F -> L (may be a predisposing factor for congenital
FT dwarfism)"
FT /evidence="ECO:0000269|PubMed:10602989"
SQ SEQUENCE 291 AA; 32504 MW; CA10C7C94D20D903 CRC64;
MSCQPFTSAD TFLPLNSEAS AALPLIMHPG AAECLPGSNH ATNVVSTATG LHYSVPSCHY
GNQPSTYGVM AGGLTPCLYK FPEHGLGPGF PAAHQPLLAE GPAVADFKQE LRRRSKLAEE
PVDTESPEIR ELEKFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF
KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS
SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FTISKEHLEC R
