ID   PIT1_MACMU              Reviewed;         291 AA.
AC   Q28503;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   25-MAY-2022, entry version 129.
DE   RecName: Full=Pituitary-specific positive transcription factor 1;
DE            Short=PIT-1;
DE   AltName: Full=Growth hormone factor 1;
DE            Short=GHF-1;
GN   Name=POU1F1; Synonyms=GHF1, PIT1;
OS   Macaca mulatta (Rhesus macaque).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=9062486; DOI=10.1210/jcem.82.3.3791;
RA   Schanke J.T., Conwell C.M., Durning M., Fisher J.M., Golos T.G.;
RT   "Pit-1/growth hormone factor 1 splice variant expression in the rhesus
RT   monkey pituitary gland and the rhesus and human placenta.";
RL   J. Clin. Endocrinol. Metab. 82:800-807(1997).
CC   -!- FUNCTION: Transcription factor involved in the specification of the
CC       lactotrope, somatotrope, and thyrotrope phenotypes in the developing
CC       anterior pituitary. Activates growth hormone and prolactin genes.
CC       Specifically binds to the consensus sequence 5'-TAAAT-3'.
CC       {ECO:0000250|UniProtKB:P28069}.
CC   -!- SUBUNIT: Interacts with PITX1. Interacts with LHX3. Interacts with
CC       ELK1. {ECO:0000250|UniProtKB:P28069}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28069}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=B; Synonyms=PIT-1B, GHF-1;
CC         IsoId=Q28503-1; Sequence=Displayed;
CC       Name=A; Synonyms=PIT-1A, GHF-2;
CC         IsoId=Q28503-2; Sequence=VSP_002315;
CC   -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC       number of yeast and animal transcription factors.
CC       {ECO:0000250|UniProtKB:P28069}.
CC   -!- MISCELLANEOUS: [Isoform A]: Altered in its ability to trans-activate
CC       compared to isoform B. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-1
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U53566; AAB51352.1; -; mRNA.
DR   RefSeq; NP_001036325.1; NM_001042860.1. [Q28503-2]
DR   AlphaFoldDB; Q28503; -.
DR   SMR; Q28503; -.
DR   STRING; 9544.ENSMMUP00000021350; -.
DR   GeneID; 719349; -.
DR   KEGG; mcc:719349; -.
DR   CTD; 5449; -.
DR   eggNOG; KOG3802; Eukaryota.
DR   InParanoid; Q28503; -.
DR   OrthoDB; 797288at2759; -.
DR   Proteomes; UP000006718; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR015586; Pit_1.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636:SF84; PTHR11636:SF84; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..291
FT                   /note="Pituitary-specific positive transcription factor 1"
FT                   /id="PRO_0000100698"
FT   DOMAIN          124..198
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        214..273
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   MOTIF           5..13
FT                   /note="9aaTAD"
FT                   /evidence="ECO:0000250|UniProtKB:P28069"
FT   VAR_SEQ         47
FT                   /note="T -> TVPSILSLIQTPKCLCTHFLVTTLGNT (in isoform A)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002315"
SQ   SEQUENCE   291 AA;  32959 MW;  79ACFBAD0C8DED15 CRC64;
     MSCQAFTSAD TFIPLNSDAS ATLPLIMHHS AAECLPVSNH ATNVMSTATG LHYSVPSCHY
     GNQPSTYGVM AGSLTLVFYK FPDHTLSHGF PPIHQPLLAE DPTAADFKQE LRRKSKLVEE
     PIDMDSPEIR ELEKFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF
     KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS
     SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R