PIT1_MELGA
ID PIT1_MELGA Reviewed; 370 AA.
AC Q05749;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Pituitary-specific positive transcription factor 1;
DE Short=PIT-1;
DE AltName: Full=Growth hormone factor 1;
DE Short=GHF-1;
GN Name=POU1F1; Synonyms=PIT1;
OS Meleagris gallopavo (Wild turkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Meleagridinae; Meleagris.
OX NCBI_TaxID=9103;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pituitary;
RX PubMed=1418622; DOI=10.1089/dna.1992.11.651;
RA Wong E.A., Silsby J.L., el Halawani M.E.;
RT "Complementary DNA cloning and expression of Pit-1/GHF-1 from the domestic
RT turkey.";
RL DNA Cell Biol. 11:651-660(1992).
RN [2]
RP SEQUENCE REVISION, AND ALTERNATIVE SPLICING.
RA Wong E.A., Sharova L., Kurima K., Weatherly K.L.;
RL Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor that activates growth hormone and
CC prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-
CC 3'. {ECO:0000250|UniProtKB:P28069}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28069}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=PIT-1beta*;
CC IsoId=Q05749-1; Sequence=Displayed;
CC Name=PIT-1;
CC IsoId=Q05749-2; Sequence=VSP_002318;
CC Name=PIT-1*;
CC IsoId=Q05749-3; Sequence=VSP_002319;
CC -!- TISSUE SPECIFICITY: Pituitary gland.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:P28069}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-1
CC subfamily. {ECO:0000305}.
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DR EMBL; U18928; AAB04690.1; -; Genomic_DNA.
DR EMBL; U62732; AAB04690.1; JOINED; Genomic_DNA.
DR EMBL; U18923; AAB04690.1; JOINED; Genomic_DNA.
DR EMBL; U18924; AAB04690.1; JOINED; Genomic_DNA.
DR EMBL; U18925; AAB04690.1; JOINED; Genomic_DNA.
DR EMBL; U18926; AAB04690.1; JOINED; Genomic_DNA.
DR EMBL; U18927; AAB04690.1; JOINED; Genomic_DNA.
DR EMBL; U18928; AAB04691.1; -; Genomic_DNA.
DR EMBL; U62732; AAB04691.1; JOINED; Genomic_DNA.
DR EMBL; U18923; AAB04691.1; JOINED; Genomic_DNA.
DR EMBL; U18924; AAB04691.1; JOINED; Genomic_DNA.
DR EMBL; U18925; AAB04691.1; JOINED; Genomic_DNA.
DR EMBL; U18926; AAB04691.1; JOINED; Genomic_DNA.
DR EMBL; U18927; AAB04691.1; JOINED; Genomic_DNA.
DR EMBL; U18928; AAB04692.1; -; Genomic_DNA.
DR EMBL; U18923; AAB04692.1; JOINED; Genomic_DNA.
DR EMBL; U18924; AAB04692.1; JOINED; Genomic_DNA.
DR EMBL; U18925; AAB04692.1; JOINED; Genomic_DNA.
DR EMBL; U18926; AAB04692.1; JOINED; Genomic_DNA.
DR EMBL; U18927; AAB04692.1; JOINED; Genomic_DNA.
DR EMBL; X69471; CAA49229.1; ALT_SEQ; mRNA.
DR PIR; S26693; S26693.
DR RefSeq; NP_001290151.1; NM_001303222.1.
DR AlphaFoldDB; Q05749; -.
DR SMR; Q05749; -.
DR GeneID; 100151749; -.
DR KEGG; mgp:100151749; -.
DR CTD; 5449; -.
DR InParanoid; Q05749; -.
DR OrthoDB; 797288at2759; -.
DR Proteomes; UP000001645; Unplaced.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR015586; Pit_1.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR PANTHER; PTHR11636:SF84; PTHR11636:SF84; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..370
FT /note="Pituitary-specific positive transcription factor 1"
FT /id="PRO_0000100704"
FT DOMAIN 202..276
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 292..351
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT MOTIF 5..13
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:P28069"
FT VAR_SEQ 1..47
FT /note="MTCQAFASSDNFVPLNSDSSPSLPLIMHHSAAECLPVSNHATSVVST -> M
FT YLESSCVFLP (in isoform PIT-1)"
FT /evidence="ECO:0000305"
FT /id="VSP_002318"
FT VAR_SEQ 48..75
FT /note="Missing (in isoform PIT-1*)"
FT /evidence="ECO:0000305"
FT /id="VSP_002319"
SQ SEQUENCE 370 AA; 41191 MW; AB2ABFCBE257C171 CRC64;
MTCQAFASSD NFVPLNSDSS PSLPLIMHHS AAECLPVSNH ATSVVSTVPS VLSLIQTPKC
SHLHFAMMTS GNVSAGLHYS VPSCHYGNQT STYGVMTGIK PATPEMLSAS LSQSRILQTC
SMPHPNVVNG VSTLQSKSFS FSCSLTPCLY KFPEHALSAS SCALGHSFTP MHQTLLSDDP
TAADFKQEFR RKSKSVEEPV DMDSPEIREL EKFANEFKLR RIKLGYTQTN VGEALAAVHG
SEFSQTTICR FENSQLSFKN ACKLKSILSK WLEEAEQVGA LYNEKVGVNE RKRKRRTTIS
IAAKEALERH FGEQSKPSSQ EIMRMAEGLN LEKEVVRVWF CNRRQREKRV KTSLHQNAFS
SIIKEHHECR
