ID   PIT1_ONCMY              Reviewed;         358 AA.
AC   Q08478;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Pituitary-specific positive transcription factor 1;
DE            Short=PIT-1;
DE   AltName: Full=Growth hormone factor 1;
DE            Short=GHF-1;
GN   Name=pou1f1; Synonyms=pit1;
OS   Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=8022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=8226986; DOI=10.1016/s0021-9258(20)80534-1;
RA   Yamada S., Hata J., Yamashita S.;
RT   "Molecular cloning of fish Pit-1 cDNA and its functional binding to
RT   promoter of gene expressed in the pituitary.";
RL   J. Biol. Chem. 268:24361-24366(1993).
CC   -!- FUNCTION: Transcription factor that activates growth hormone and
CC       prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-
CC       3'. {ECO:0000250|UniProtKB:P28069}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28069}.
CC   -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC       number of yeast and animal transcription factors.
CC       {ECO:0000250|UniProtKB:P28069}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; D16513; BAA03964.1; -; mRNA.
DR   PIR; A49511; A49511.
DR   RefSeq; NP_001118118.1; NM_001124646.1.
DR   AlphaFoldDB; Q08478; -.
DR   SMR; Q08478; -.
DR   Ensembl; ENSOMYT00000078026; ENSOMYP00000071664; ENSOMYG00000033132.
DR   GeneID; 100136675; -.
DR   KEGG; omy:100136675; -.
DR   CTD; 5449; -.
DR   GeneTree; ENSGT00940000158913; -.
DR   OrthoDB; 797288at2759; -.
DR   GO; GO:0005634; C:nucleus; IDA:AgBase.
DR   GO; GO:0001046; F:core promoter sequence-specific DNA binding; IDA:AgBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR   GO; GO:0010628; P:positive regulation of gene expression; IDA:AgBase.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR015586; Pit_1.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636:SF84; PTHR11636:SF84; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Homeobox; Nucleus; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..358
FT                   /note="Pituitary-specific positive transcription factor 1"
FT                   /id="PRO_0000100706"
FT   DOMAIN          192..266
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        282..341
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          164..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           5..12
FT                   /note="9aaTAD"
FT                   /evidence="ECO:0000250|UniProtKB:P28069"
FT   COMPBIAS        175..195
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   358 AA;  39376 MW;  DD7696FC6A6B1967 CRC64;
     MSCQAFSADS FTTLAGDSLP LLMHHASAAD CLPSSASTHT HNMVSAVPSG LSLLQSSKRS
     HMHLSTSTLG NALSNGPPGL HYPVTPCHYS NQQTTYGMMA AQEMLSASIS QTRILQTCSV
     PHPNMVNGAN TLQGSLAPCL YKFPEHGLGG GSCSLSHSFP PLPPAVLSEE PPLGGTKDLR
     LRSRPPDDPP DMDSPQIREL EKFANDFKLR RIKLGYTQTN VGEALAAVHG SEFSQTTICR
     FENLQLSFKN ACTLKAILAK WLDEAEQAGA LFNEKMGMNE RKRKRRTTIS LGAKEALERS
     FGEKIKPSSQ EIVRMAEGLH LEKEVVRVWF CNRRQREKRV KTSLHHSSYL TKDSPTYR