PIT1_PIG
ID PIT1_PIG Reviewed; 291 AA.
AC Q04788; O18807; O46410;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 3.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=Pituitary-specific positive transcription factor 1;
DE Short=PIT-1;
DE AltName: Full=Growth hormone factor 1;
DE Short=GHF-1;
GN Name=POU1F1; Synonyms=PIT-1, PIT1;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pituitary;
RA Vila V., Sanchez M.P., Malagon M.M., Gracia F., Castrillo J.-L.;
RT "Molecular cloning of pig (Sus scrofa) pituitary transcription factor GHF-
RT 1/Pit-1.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yu T.-P., Sun H.S., Rothschild M.F., Tuggle C.K.;
RT "Cloning of the full length pig PIT-1 cDNA and its alternative transcripts,
RT and functional studies of their encoded proteins.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 139-267.
RC STRAIN=Duroc; TISSUE=Brain cortex, and Pituitary;
RX PubMed=8098921; DOI=10.1111/j.1365-2052.1993.tb00913.x;
RA Tuggle C.K., Yu T.-P., Helm J., Rothschild M.F.;
RT "Cloning and restriction fragment length polymorphism analysis of a cDNA
RT for swine PIT-1, a gene controlling growth hormone expression.";
RL Anim. Genet. 24:17-21(1993).
CC -!- FUNCTION: Transcription factor involved in the specification of the
CC lactotrope, somatotrope, and thyrotrope phenotypes in the developing
CC anterior pituitary. Activates growth hormone and prolactin genes.
CC Specifically binds to the consensus sequence 5'-TAAAT-3'.
CC {ECO:0000250|UniProtKB:P28069}.
CC -!- SUBUNIT: Interacts with PITX1. Interacts with LHX3. Interacts with
CC ELK1. {ECO:0000250|UniProtKB:P28069}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28069}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:P28069}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-1
CC subfamily. {ECO:0000305}.
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DR EMBL; Y13617; CAA73939.1; -; mRNA.
DR EMBL; AF016251; AAB65789.2; -; mRNA.
DR EMBL; L03841; AAA31108.1; -; mRNA.
DR RefSeq; NP_999328.1; NM_214163.1.
DR AlphaFoldDB; Q04788; -.
DR SMR; Q04788; -.
DR STRING; 9823.ENSSSCP00000012779; -.
DR PaxDb; Q04788; -.
DR PRIDE; Q04788; -.
DR GeneID; 397325; -.
DR KEGG; ssc:397325; -.
DR CTD; 5449; -.
DR eggNOG; KOG3802; Eukaryota.
DR InParanoid; Q04788; -.
DR OrthoDB; 797288at2759; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR015586; Pit_1.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR PANTHER; PTHR11636:SF84; PTHR11636:SF84; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..291
FT /note="Pituitary-specific positive transcription factor 1"
FT /id="PRO_0000100700"
FT DOMAIN 124..198
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 214..273
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT MOTIF 5..13
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:P28069"
FT CONFLICT 64
FT /note="P -> S (in Ref. 2; AAB65789)"
FT /evidence="ECO:0000305"
FT CONFLICT 94
FT /note="Y -> H (in Ref. 2; AAB65789)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="P -> A (in Ref. 2; AAB65789)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="V -> A (in Ref. 1; CAA73939)"
FT /evidence="ECO:0000305"
FT CONFLICT 144
FT /note="I -> M (in Ref. 3; AAA31108)"
FT /evidence="ECO:0000305"
FT CONFLICT 155..161
FT /note="EALAAVH -> QVAAAWT (in Ref. 1; CAA73939)"
FT /evidence="ECO:0000305"
FT CONFLICT 183
FT /note="A -> R (in Ref. 1; CAA73939)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="E -> V (in Ref. 1; CAA73939)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="R -> S (in Ref. 3; AAA31108)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="V -> F (in Ref. 1; CAA73939)"
FT /evidence="ECO:0000305"
FT CONFLICT 265
FT /note="R -> H (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 291 AA; 33033 MW; C13EB6634697F8EA CRC64;
MSCQPFTSAD TFIPLNSESS ATLPLIMHHS AAECLPASNH ATNVMSTATG LHYSVPSCHY
GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPMYQPLLPE DPTAADFKQE LRRKSKLVEE
PIDMDSPEIR ELEKFPNEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF
KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS
SQEILRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FTISKEHLEC R
