PIT1_USTMA
ID PIT1_USTMA Reviewed; 435 AA.
AC A0A0D1E6B3;
DT 31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2015, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=Virulence factor PIT1 {ECO:0000303|PubMed:17080091};
DE AltName: Full=Proteins important for tumors 1 {ECO:0000303|PubMed:21692877};
GN Name=PIT1 {ECO:0000303|PubMed:21692877}; ORFNames=UMAG_01374;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP INDUCTION, FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=21692877; DOI=10.1111/j.1365-2958.2011.07728.x;
RA Doehlemann G., Reissmann S., Assmann D., Fleckenstein M., Kahmann R.;
RT "Two linked genes encoding a secreted effector and a membrane protein are
RT essential for Ustilago maydis-induced tumour formation.";
RL Mol. Microbiol. 81:751-766(2011).
RN [4]
RP GLYCOSYLATION.
RX PubMed=22416226; DOI=10.1371/journal.ppat.1002563;
RA Fernandez-Alvarez A., Marin-Menguiano M., Lanver D., Jimenez-Martin A.,
RA Elias-Villalobos A., Perez-Pulido A.J., Kahmann R., Ibeas J.I.;
RT "Identification of O-mannosylated virulence factors in Ustilago maydis.";
RL PLoS Pathog. 8:E1002563-E1002563(2012).
RN [5]
RP INDUCTION.
RX PubMed=27093436; DOI=10.1371/journal.pone.0153861;
RA Hampel M., Jakobi M., Schmitz L., Meyer U., Finkernagel F., Doehlemann G.,
RA Heimel K.;
RT "Unfolded protein response (UPR) regulator Cib1 controls expression of
RT genes encoding secreted virulence factors in Ustilago maydis.";
RL PLoS ONE 11:E0153861-E0153861(2016).
CC -!- FUNCTION: Plasma membrane virulence factor required for spreading and
CC inducing tumors in infected leaves. {ECO:0000269|PubMed:21692877}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:21692877};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expression is highly up-regulated during all stages of
CC pathogenic development (PubMed:21692877). Expression is positively
CC regulated by the unfolded protein response (UPR) signaling via the
CC binding of CIB1 to the UPRE motif localized at the PIT1/PIT2 promoter
CC (PubMed:27093436). {ECO:0000269|PubMed:21692877,
CC ECO:0000269|PubMed:27093436}.
CC -!- PTM: O-mannosylated by PMT4 (PubMed:22416226). Is also N-glycosylated
CC (PubMed:22416226). {ECO:0000269|PubMed:22416226}.
CC -!- DISRUPTION PHENOTYPE: Keeps the ability to penetrate maize epidermis
CC and to grow intracellularly at sites of infection but fails to spread
CC and induce tumors in infected leaves. {ECO:0000269|PubMed:21692877}.
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DR EMBL; CM003141; KIS71479.1; -; Genomic_DNA.
DR RefSeq; XP_011387263.1; XM_011388961.1.
DR AlphaFoldDB; A0A0D1E6B3; -.
DR EnsemblFungi; KIS71479; KIS71479; UMAG_01374.
DR GeneID; 23562419; -.
DR KEGG; uma:UMAG_01374; -.
DR VEuPathDB; FungiDB:UMAG_01374; -.
DR eggNOG; ENOG502R2GG; Eukaryota.
DR OMA; PNMAEIF; -.
DR OrthoDB; 945646at2759; -.
DR Proteomes; UP000000561; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..435
FT /note="Virulence factor PIT1"
FT /id="PRO_0000447640"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 392..435
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..407
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..435
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 330
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 435 AA; 47743 MW; F15DF06AFE0ECCF6 CRC64;
MERHDGEEYI PAIFGGQPPH ASQVISLMKF SQETTTWNLP IMIAQALLVS EVIRTYPAEL
RMLHRLFQRK HPNMAEIFFI LIKYLTIFAV IFDILVTETF AARTDFDCRS WAWTSSTFYF
MCSTLVFCVI GWRARIIFRT SQVASWSLSV GLMGQFAVAM WTNYRVDKAD ALTPAGTCAP
AAQVHADASQ RNAALQLHFW QSSTFWFLLY NTIFESSILM ACCIKLRKTS SGPSGLTKIA
KVLFSNNVHY MAGVETCNVI ELVMLLGWTS SLPPVHITSI AIQIVVGLQM LIGEQEAVYS
PTCSQLSYSQ YSSDSGGYIN KHHAATSSSN GTFSTPSRTL SYVKRPGTGT TVADIGCADA
SGGGQHGRKG TFSSISSVPA YVKANPIVET VSPQMPSKAQ SQSIPYKREV EVTVDMSPVP
PPPGPSPAPL PAPYM
