ID   PITA_ECOL6              Reviewed;         499 AA.
AC   P0AFJ8; P37308;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Low-affinity inorganic phosphate transporter 1;
GN   Name=pitA; OrderedLocusNames=c4291;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Low-affinity inorganic phosphate transport. Can also
CC       transport arsenate (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC       2.A.20) family. Pit subfamily. {ECO:0000305}.
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DR   EMBL; AE014075; AAN82727.1; -; Genomic_DNA.
DR   RefSeq; WP_000902780.1; NC_004431.1.
DR   AlphaFoldDB; P0AFJ8; -.
DR   SMR; P0AFJ8; -.
DR   STRING; 199310.c4291; -.
DR   EnsemblBacteria; AAN82727; AAN82727; c4291.
DR   GeneID; 66672625; -.
DR   KEGG; ecc:c4291; -.
DR   eggNOG; COG0306; Bacteria.
DR   HOGENOM; CLU_015355_4_0_6; -.
DR   OMA; GYDITRT; -.
DR   BioCyc; ECOL199310:C4291-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR001204; Phos_transporter.
DR   PANTHER; PTHR11101; PTHR11101; 1.
DR   Pfam; PF01384; PHO4; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Phosphate transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..499
FT                   /note="Low-affinity inorganic phosphate transporter 1"
FT                   /id="PRO_0000080784"
FT   TOPO_DOM        1..4
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..51
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        73..93
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        115..123
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        145..154
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        176..206
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        228..232
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        233..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        254..381
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        382..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        403..429
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        430..450
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        451..472
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        473..493
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        494..499
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   499 AA;  53389 MW;  93D551131C524084 CRC64;
     MLHLFAGLDL HTGLLLLLAL AFVLFYEAIN GFHDTANAVA TVIYTRAMRS QLAVVMAAVF
     NFLGVLLGGL SVAYAIVHML PTDLLLNMGS SHGLAMVFSM LLAAIIWNLG TWYFGLPASS
     SHTLIGAIIG IGLTNALMTG TSVVDALNIP KVLSIFGSLI VSPIVGLVFA GGLIFLLRRY
     WSGTKKRARI HLTPAEREKK DGKKKPPFWT RIALILSAIG VAFSHGANDG QKGIGLVMLV
     LIGVAPAGFV VNMNATGYEI TRTRDAINNV EAYFEQHPAL LKQATGADQL VPAPEAGATQ
     PAEFHCHPSN TINALNRLKG MLTTDVESYD KLSLDQRSQM RRIMLCVSDT IDKVVKMPGV
     SADDQRLLKK LKSDMLSTIE YAPVWIIMAV ALALGIGTMI GWRRVATTIG EKIGKKGMTY
     AQGMSAQMTA AVSIGLASYT GMPVSTTHVL SSSVAGTMVV DGGGLQRKTV TSILMAWVFT
     LPAAVLLSGG LYWLSLQFL