PITA_ECOLI
ID PITA_ECOLI Reviewed; 499 AA.
AC P0AFJ7; P37308; Q2M7F5;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Low-affinity inorganic phosphate transporter 1;
GN Name=pitA; Synonyms=pit; OrderedLocusNames=b3493, JW3460;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Low-affinity inorganic phosphate transport. Can also
CC transport arsenate.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC 2.A.20) family. Pit subfamily. {ECO:0000305}.
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DR EMBL; U00039; AAB18469.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76518.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77801.1; -; Genomic_DNA.
DR PIR; S47713; S47713.
DR RefSeq; NP_417950.1; NC_000913.3.
DR RefSeq; WP_000902780.1; NZ_STEB01000046.1.
DR AlphaFoldDB; P0AFJ7; -.
DR SMR; P0AFJ7; -.
DR BioGRID; 4262094; 5.
DR STRING; 511145.b3493; -.
DR TCDB; 2.A.20.1.1; the inorganic phosphate transporter (pit) family.
DR jPOST; P0AFJ7; -.
DR PaxDb; P0AFJ7; -.
DR PRIDE; P0AFJ7; -.
DR EnsemblBacteria; AAC76518; AAC76518; b3493.
DR EnsemblBacteria; BAE77801; BAE77801; BAE77801.
DR GeneID; 66672625; -.
DR GeneID; 948009; -.
DR KEGG; ecj:JW3460; -.
DR KEGG; eco:b3493; -.
DR PATRIC; fig|1411691.4.peg.3229; -.
DR EchoBASE; EB2142; -.
DR eggNOG; COG0306; Bacteria.
DR HOGENOM; CLU_015355_4_0_6; -.
DR InParanoid; P0AFJ7; -.
DR OMA; GYDITRT; -.
DR PhylomeDB; P0AFJ7; -.
DR BioCyc; EcoCyc:PITA-MON; -.
DR BioCyc; MetaCyc:PITA-MON; -.
DR PRO; PR:P0AFJ7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015295; F:solute:proton symporter activity; IDA:EcoCyc.
DR GO; GO:0015654; F:tellurite transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0010960; P:magnesium ion homeostasis; IDA:EcoCyc.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IMP:EcoCyc.
DR GO; GO:0015710; P:tellurite transport; IMP:EcoCyc.
DR InterPro; IPR001204; Phos_transporter.
DR PANTHER; PTHR11101; PTHR11101; 1.
DR Pfam; PF01384; PHO4; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Phosphate transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..499
FT /note="Low-affinity inorganic phosphate transporter 1"
FT /id="PRO_0000080782"
FT TOPO_DOM 1..4
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..51
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..93
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..154
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..206
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..232
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 403..429
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 451..472
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 473..493
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 494..499
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 499 AA; 53389 MW; 93D551131C524084 CRC64;
MLHLFAGLDL HTGLLLLLAL AFVLFYEAIN GFHDTANAVA TVIYTRAMRS QLAVVMAAVF
NFLGVLLGGL SVAYAIVHML PTDLLLNMGS SHGLAMVFSM LLAAIIWNLG TWYFGLPASS
SHTLIGAIIG IGLTNALMTG TSVVDALNIP KVLSIFGSLI VSPIVGLVFA GGLIFLLRRY
WSGTKKRARI HLTPAEREKK DGKKKPPFWT RIALILSAIG VAFSHGANDG QKGIGLVMLV
LIGVAPAGFV VNMNATGYEI TRTRDAINNV EAYFEQHPAL LKQATGADQL VPAPEAGATQ
PAEFHCHPSN TINALNRLKG MLTTDVESYD KLSLDQRSQM RRIMLCVSDT IDKVVKMPGV
SADDQRLLKK LKSDMLSTIE YAPVWIIMAV ALALGIGTMI GWRRVATTIG EKIGKKGMTY
AQGMSAQMTA AVSIGLASYT GMPVSTTHVL SSSVAGTMVV DGGGLQRKTV TSILMAWVFT
LPAAVLLSGG LYWLSLQFL
