PITB_ECOLI
ID PITB_ECOLI Reviewed; 499 AA.
AC P43676; Q2M9K8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Probable low-affinity inorganic phosphate transporter 2;
GN Name=pitB; OrderedLocusNames=b2987, JW2955;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-217.
RC STRAIN=K12;
RX PubMed=7775463; DOI=10.1074/jbc.270.23.14031;
RA Bollinger J.M. Jr., Kwon D.S., Huisman G.W., Kolter R., Walsh C.T.;
RT "Glutathionylspermidine metabolism in Escherichia coli. Purification,
RT cloning, overproduction, and characterization of a bifunctional
RT glutathionylspermidine synthetase/amidase.";
RL J. Biol. Chem. 270:14031-14041(1995).
RN [4]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Low-affinity inorganic phosphate transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC 2.A.20) family. Pit subfamily. {ECO:0000305}.
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DR EMBL; U28377; AAA69154.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76023.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77048.1; -; Genomic_DNA.
DR EMBL; U23148; AAC43340.1; -; Genomic_DNA.
DR PIR; A65085; A65085.
DR RefSeq; NP_417461.1; NC_000913.3.
DR RefSeq; WP_000933250.1; NZ_SSZK01000023.1.
DR AlphaFoldDB; P43676; -.
DR SMR; P43676; -.
DR BioGRID; 4261181; 20.
DR STRING; 511145.b2987; -.
DR TCDB; 2.A.20.1.2; the inorganic phosphate transporter (pit) family.
DR PaxDb; P43676; -.
DR PRIDE; P43676; -.
DR EnsemblBacteria; AAC76023; AAC76023; b2987.
DR EnsemblBacteria; BAE77048; BAE77048; BAE77048.
DR GeneID; 947475; -.
DR KEGG; ecj:JW2955; -.
DR KEGG; eco:b2987; -.
DR PATRIC; fig|1411691.4.peg.3743; -.
DR EchoBASE; EB2721; -.
DR eggNOG; COG0306; Bacteria.
DR HOGENOM; CLU_015355_4_0_6; -.
DR InParanoid; P43676; -.
DR OMA; RANPHKA; -.
DR PhylomeDB; P43676; -.
DR BioCyc; EcoCyc:PITB-MON; -.
DR BioCyc; MetaCyc:PITB-MON; -.
DR PRO; PR:P43676; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IDA:EcoCyc.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IMP:EcoCyc.
DR InterPro; IPR001204; Phos_transporter.
DR PANTHER; PTHR11101; PTHR11101; 1.
DR Pfam; PF01384; PHO4; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Phosphate transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..499
FT /note="Probable low-affinity inorganic phosphate
FT transporter 2"
FT /id="PRO_0000080785"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 473..493
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 182
FT /note="S -> C (in Ref. 3; AAC43340)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="A -> G (in Ref. 3; AAC43340)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 499 AA; 53809 MW; 1D3677057FAB295B CRC64;
MLNLFVGLDI YTGLLLLLAL AFVLFYEAIN GFHDTANAVA AVIYTRAMQP QLAVVMAAFF
NFFGVLLGGL SVAYAIVHML PTDLLLNMGS THGLAMVFSM LLAAIIWNLG TWFFGLPASS
SHTLIGAIIG IGLTNALLTG SSVMDALNLR EVTKIFSSLI VSPIVGLVIA GGLIFLLRRY
WSGTKKRDRI HRIPEDRKKK KGKRKPPFWT RIALIVSAAG VAFSHGANDG QKGIGLVMLV
LVGIAPAGFV VNMNASGYEI TRTRDAVTNF EHYLQQHPEL PQKLIAMEPP LPAASTDGTQ
VTEFHCHPAN TFDAIARVKT MLPGNMESYE PLSVSQRSQL RRIMLCISDT SAKLAKLPGV
SKEDQNLLKK LRSDMLSTIE YAPVWIIMAV ALALGIGTMI GWRRVAMTIG EKIGKRGMTY
AQGMAAQMTA AVSIGLASYI GMPVSTTHVL SSAVAGTMVV DGGGLQRKTV TSILMAWVFT
LPAAIFLSGG LYWIALQLI
