PITX1_MOUSE
ID PITX1_MOUSE Reviewed; 315 AA.
AC P70314; O54742; P70427;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Pituitary homeobox 1;
DE AltName: Full=Hindlimb-expressed homeobox protein backfoot;
DE AltName: Full=Homeobox protein P-OTX;
DE AltName: Full=Homeobox protein PITX1;
DE AltName: Full=Paired-like homeodomain transcription factor 1;
DE AltName: Full=Pituitary OTX-related factor;
GN Name=Pitx1; Synonyms=Bft, Potx, Ptx1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pituitary;
RX PubMed=8755540; DOI=10.1073/pnas.93.15.7706;
RA Szeto D.P., Ryan A.K., O'Connell S.M., Rosenfeld M.G.;
RT "P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior
RT pituitary gland development.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:7706-7710(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pituitary;
RX PubMed=8675014; DOI=10.1101/gad.10.10.1284;
RA Lamonerie T., Tremblay J.J., Lanctot C., Therrien M., Gauthier Y.,
RA Drouin J.;
RT "Ptx1, a bicoid-related homeo box transcription factor involved in
RT transcription of the pro-opiomelanocortin gene.";
RL Genes Dev. 10:1284-1295(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 133-315.
RX PubMed=9070926; DOI=10.1006/geno.1996.4558;
RA Shang J., Li X., Ring H.Z., Clayton D.A., Francke U.;
RT "Backfoot, a novel homeobox gene, maps to human chromosome 5 (BFT) and
RT mouse chromosome 13 (Bft).";
RL Genomics 40:108-113(1997).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=9226452; DOI=10.1242/dev.124.14.2807;
RA Lanctot C., Lamolet B., Drouin J.;
RT "The bicoid-related homeoprotein Ptx1 defines the most anterior domain of
RT the embryo and differentiates posterior from anterior lateral mesoderm.";
RL Development 124:2807-2817(1997).
CC -!- FUNCTION: Sequence-specific transcription factor that binds gene
CC promoters and activates their transcription. May play a role in the
CC development of anterior structures, and in particular, the brain and
CC facies and in specifying the identity or structure of hindlimb. Can
CC independently activate and synergize with PIT-1 on pituitary-specific
CC target gene promoters, thus may subserve functions in generating both
CC precursor and specific cell phenotypes in the anterior pituitary gland
CC and in several other organs. Can activate pituitary transcription of
CC the proopiomelanocortin gene. {ECO:0000250|UniProtKB:P56673,
CC ECO:0000269|PubMed:8675014, ECO:0000269|PubMed:8755540}.
CC -!- SUBUNIT: Interacts with POU1F1. {ECO:0000250|UniProtKB:P78337}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in primordial Rathke pouch, oral
CC epithelium, first branchial arch, duodenum, and hindlimb.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout pituitary development. Not
CC found in 8 dpc embryos but seen in embryos at 8.5 dpc in the region of
CC the first branchial arch and ventral portion of the caudal-most region
CC of the embryo. At 9.5 dpc observed in the hindlimb buds, maxillary and
CC mandibular components of the first branchial arch and Rathke pouch and
CC the pattern of expression continues through 10.5 dpc at 11.5 dpc also
CC found in ventral mesenchyme covering the abdominal cavity and in the
CC nasal epithelium. At 17.5 dpc expressed in derivatives of the first
CC branchial arch, including tongue and mandible as well as duodenum,
CC salivary glands, nasal epithelium and condensing cartilage in the
CC hindlimbs. {ECO:0000269|PubMed:9226452}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; U54499; AAC52716.1; -; mRNA.
DR EMBL; U71206; AAB16860.1; -; mRNA.
DR EMBL; BC012696; AAH12696.1; -; mRNA.
DR EMBL; U70371; AAC53059.1; -; mRNA.
DR CCDS; CCDS26556.1; -.
DR RefSeq; NP_035227.1; NM_011097.2.
DR RefSeq; XP_006517220.1; XM_006517157.3.
DR RefSeq; XP_006517221.1; XM_006517158.3.
DR AlphaFoldDB; P70314; -.
DR SMR; P70314; -.
DR BioGRID; 202186; 4.
DR STRING; 10090.ENSMUSP00000021968; -.
DR PhosphoSitePlus; P70314; -.
DR PaxDb; P70314; -.
DR PRIDE; P70314; -.
DR ProteomicsDB; 288174; -.
DR Antibodypedia; 14722; 236 antibodies from 33 providers.
DR DNASU; 18740; -.
DR Ensembl; ENSMUST00000021968; ENSMUSP00000021968; ENSMUSG00000021506.
DR GeneID; 18740; -.
DR KEGG; mmu:18740; -.
DR UCSC; uc007qsd.1; mouse.
DR CTD; 5307; -.
DR MGI; MGI:107374; Pitx1.
DR VEuPathDB; HostDB:ENSMUSG00000021506; -.
DR eggNOG; KOG0486; Eukaryota.
DR GeneTree; ENSGT00940000154518; -.
DR HOGENOM; CLU_030301_0_0_1; -.
DR InParanoid; P70314; -.
DR OMA; QASHDMA; -.
DR OrthoDB; 1432356at2759; -.
DR PhylomeDB; P70314; -.
DR TreeFam; TF351940; -.
DR BioGRID-ORCS; 18740; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Pitx1; mouse.
DR PRO; PR:P70314; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P70314; protein.
DR Bgee; ENSMUSG00000021506; Expressed in mouth mucosa and 148 other tissues.
DR ExpressionAtlas; P70314; baseline and differential.
DR Genevisible; P70314; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0014707; P:branchiomeric skeletal muscle development; IMP:MGI.
DR GO; GO:0051216; P:cartilage development; IMP:MGI.
DR GO; GO:0035116; P:embryonic hindlimb morphogenesis; IMP:CACAO.
DR GO; GO:0035137; P:hindlimb morphogenesis; IDA:MGI.
DR GO; GO:0048625; P:myoblast fate commitment; IMP:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:CACAO.
DR GO; GO:0021983; P:pituitary gland development; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Activator; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..315
FT /note="Pituitary homeobox 1"
FT /id="PRO_0000049219"
FT DNA_BIND 90..149
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..280
FT /note="Interaction with PIT-1"
FT MOTIF 281..294
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 287..291
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 45..66
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P78337"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P78337"
FT MOD_RES 48
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P78337"
FT CONFLICT 287
FT /note="R -> A (in Ref. 1; AAC52716)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 315 AA; 34075 MW; 6E1C24718DCA3F30 CRC64;
MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES SDADLPDKER
GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ QLQELEATFQ RNRYPDMSMR
EEIAVWTNLT EPRVRVWFKN RRAKWRKRER NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY
SYNNWAAKSL APAPLSTKSF TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN
SGLNNINNLT GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG
LQGPASGLNA CQYNS
