PITX1_RAT
ID PITX1_RAT Reviewed; 315 AA.
AC Q99NA7;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Pituitary homeobox 1;
DE AltName: Full=Homeobox protein PITX1;
DE AltName: Full=Paired-like homeodomain transcription factor 1;
GN Name=Pitx1; Synonyms=Ptx1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Pituitary;
RX PubMed=11250922; DOI=10.1210/endo.142.4.8098;
RA Kimura N., Tomizawa S., Arai K.N., Osamura R.Y., Kimura N.;
RT "Characterization of 5'-flanking region of rat somatostatin receptor sst2
RT gene: transcriptional regulatory elements and activation by Pitx1 and
RT estrogen.";
RL Endocrinology 142:1427-1441(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Sequence-specific transcription factor that binds gene
CC promoters and activates their transcription. May play a role in the
CC development of anterior structures, and in particular, the brain and
CC facies and in specifying the identity or structure of hindlimb.
CC {ECO:0000250|UniProtKB:P78337}.
CC -!- SUBUNIT: Interacts with POU1F1. {ECO:0000250|UniProtKB:P56673}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AB047298; BAB41202.1; -; mRNA.
DR EMBL; BC061966; AAH61966.1; -; mRNA.
DR RefSeq; NP_446076.1; NM_053624.2.
DR RefSeq; XP_006253631.1; XM_006253569.3.
DR AlphaFoldDB; Q99NA7; -.
DR SMR; Q99NA7; -.
DR STRING; 10116.ENSRNOP00000015307; -.
DR iPTMnet; Q99NA7; -.
DR PhosphoSitePlus; Q99NA7; -.
DR PaxDb; Q99NA7; -.
DR Ensembl; ENSRNOT00000015307; ENSRNOP00000015307; ENSRNOG00000011423.
DR GeneID; 113983; -.
DR KEGG; rno:113983; -.
DR UCSC; RGD:69253; rat.
DR CTD; 5307; -.
DR RGD; 69253; Pitx1.
DR eggNOG; KOG0486; Eukaryota.
DR GeneTree; ENSGT00940000154518; -.
DR HOGENOM; CLU_030301_0_0_1; -.
DR InParanoid; Q99NA7; -.
DR OMA; QASHDMA; -.
DR OrthoDB; 1432356at2759; -.
DR PhylomeDB; Q99NA7; -.
DR TreeFam; TF351940; -.
DR PRO; PR:Q99NA7; -.
DR Proteomes; UP000002494; Chromosome 17.
DR Bgee; ENSRNOG00000011423; Expressed in esophagus and 7 other tissues.
DR Genevisible; Q99NA7; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0014707; P:branchiomeric skeletal muscle development; ISO:RGD.
DR GO; GO:0051216; P:cartilage development; ISO:RGD.
DR GO; GO:0035116; P:embryonic hindlimb morphogenesis; ISO:RGD.
DR GO; GO:0035137; P:hindlimb morphogenesis; ISO:RGD.
DR GO; GO:0048625; P:myoblast fate commitment; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0021983; P:pituitary gland development; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:RGD.
DR GO; GO:0001501; P:skeletal system development; ISO:RGD.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Activator; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..315
FT /note="Pituitary homeobox 1"
FT /id="PRO_0000049220"
FT DNA_BIND 89..148
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..280
FT /note="Interaction with PIT-1"
FT /evidence="ECO:0000250"
FT MOTIF 281..294
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 287..291
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 45..66
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P78337"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P78337"
FT MOD_RES 48
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P78337"
SQ SEQUENCE 315 AA; 34207 MW; BACBA7E43C0A9B1F CRC64;
MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARTADPR EPLENSASES SDADLPDKER
GGEAKVPEDG GAGSAGCGSA EDPAKKKKQR RQRTHFTSQQ LQELEATFQR NRYPDMSMRE
EIAVWTNLTE PRVRVWFKNR RAKWRKRERN QQLDLCKGGY VPQFSGLVQP YEDVYAAAGY
SYNNWAAKSL APAPLSTKSF TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGSVPGMPN
SGLNNINNLT GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG
LQGPASGLNA CQYNS
