PITX2_RAT
ID PITX2_RAT Reviewed; 324 AA.
AC Q9R0W1;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Pituitary homeobox 2 {ECO:0000305};
DE AltName: Full=Homeobox protein PITX2;
DE AltName: Full=Paired-like homeodomain transcription factor 2;
DE AltName: Full=rPtx2;
GN Name=Pitx2 {ECO:0000312|RGD:3331}; Synonyms=Ptx2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PTX2B).
RC STRAIN=Sprague-Dawley;
RX PubMed=9748586; DOI=10.1016/s0165-3806(98)00110-2;
RA Lindberg C., Wunderlich M., Ratliff J., Dinsmore J., Jacoby D.B.;
RT "Regulated expression of the homeobox gene, rPtx2, in the developing rat.";
RL Brain Res. Dev. Brain Res. 110:215-226(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PTX2A AND PTX2B).
RX PubMed=11032870; DOI=10.1046/j.1471-4159.2000.0751818.x;
RA Smidt M.P., Cox J.J., van Schaick H.S.A., Coolen M., Schepers J.,
RA van der Kleij A.M., Burbach J.P.H.;
RT "Analysis of three Ptx2 splice variants on transcriptional activity and
RT differential expression pattern in the brain.";
RL J. Neurochem. 75:1818-1825(2000).
CC -!- FUNCTION: Controls cell proliferation in a tissue-specific manner and
CC is involved in morphogenesis. During embryonic development, exerts a
CC role in the expansion of muscle progenitors. May play a role in the
CC proper localization of asymmetric organs such as the heart and stomach
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with PITX2. {ECO:0000250|UniProtKB:Q99697}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=PTX2A;
CC IsoId=Q9R0W1-1; Sequence=Displayed;
CC Name=PTX2B;
CC IsoId=Q9R0W1-2; Sequence=VSP_002264, VSP_002265;
CC -!- TISSUE SPECIFICITY: Expressed in the brain.
CC -!- PTM: Phosphorylation at Thr-97 impairs its association with the CCND1
CC mRNA-stabilizing complex thus shortening the half-life of CCND1.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AF039832; AAC70909.1; -; mRNA.
DR EMBL; AJ222971; CAB52283.1; -; mRNA.
DR EMBL; AJ222972; CAB52284.1; -; mRNA.
DR RefSeq; NP_001035970.1; NM_001042505.1. [Q9R0W1-1]
DR RefSeq; NP_062207.1; NM_019334.2. [Q9R0W1-2]
DR RefSeq; XP_017446543.1; XM_017591054.1. [Q9R0W1-2]
DR AlphaFoldDB; Q9R0W1; -.
DR SMR; Q9R0W1; -.
DR STRING; 10116.ENSRNOP00000046259; -.
DR PaxDb; Q9R0W1; -.
DR Ensembl; ENSRNOT00000044232; ENSRNOP00000043402; ENSRNOG00000010681. [Q9R0W1-2]
DR Ensembl; ENSRNOT00000051009; ENSRNOP00000046259; ENSRNOG00000010681. [Q9R0W1-1]
DR GeneID; 54284; -.
DR KEGG; rno:54284; -.
DR UCSC; RGD:3331; rat. [Q9R0W1-1]
DR CTD; 5308; -.
DR RGD; 3331; Pitx2.
DR eggNOG; KOG0486; Eukaryota.
DR GeneTree; ENSGT00940000154518; -.
DR HOGENOM; CLU_030301_0_0_1; -.
DR InParanoid; Q9R0W1; -.
DR OMA; NSMRNPL; -.
DR OrthoDB; 1432356at2759; -.
DR PhylomeDB; Q9R0W1; -.
DR TreeFam; TF351940; -.
DR PRO; PR:Q9R0W1; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000010681; Expressed in esophagus and 10 other tissues.
DR Genevisible; Q9R0W1; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0031490; F:chromatin DNA binding; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:RGD.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:RGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:RGD.
DR GO; GO:0009653; P:anatomical structure morphogenesis; ISO:RGD.
DR GO; GO:0009887; P:animal organ morphogenesis; ISO:RGD.
DR GO; GO:0055009; P:atrial cardiac muscle tissue morphogenesis; ISO:RGD.
DR GO; GO:0003171; P:atrioventricular valve development; ISO:RGD.
DR GO; GO:0007420; P:brain development; IEP:RGD.
DR GO; GO:0001569; P:branching involved in blood vessel morphogenesis; ISO:RGD.
DR GO; GO:0043010; P:camera-type eye development; ISO:RGD.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; ISO:RGD.
DR GO; GO:0048738; P:cardiac muscle tissue development; ISO:RGD.
DR GO; GO:0003253; P:cardiac neural crest cell migration involved in outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0061325; P:cell proliferation involved in outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0035993; P:deltoid tuberosity development; ISO:RGD.
DR GO; GO:0007368; P:determination of left/right symmetry; ISO:RGD.
DR GO; GO:0055123; P:digestive system development; ISO:RGD.
DR GO; GO:0031076; P:embryonic camera-type eye development; ISO:RGD.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISO:RGD.
DR GO; GO:0060971; P:embryonic heart tube left/right pattern formation; ISO:RGD.
DR GO; GO:0035116; P:embryonic hindlimb morphogenesis; ISO:RGD.
DR GO; GO:0061031; P:endodermal digestive tract morphogenesis; ISO:RGD.
DR GO; GO:0002074; P:extraocular skeletal muscle development; ISO:RGD.
DR GO; GO:0008585; P:female gonad development; IEP:RGD.
DR GO; GO:0007507; P:heart development; ISO:RGD.
DR GO; GO:0021855; P:hypothalamus cell migration; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0061072; P:iris morphogenesis; ISO:RGD.
DR GO; GO:0060460; P:left lung morphogenesis; ISO:RGD.
DR GO; GO:0070986; P:left/right axis specification; ISO:RGD.
DR GO; GO:0030324; P:lung development; ISO:RGD.
DR GO; GO:0008584; P:male gonad development; IEP:RGD.
DR GO; GO:0030182; P:neuron differentiation; IEP:RGD.
DR GO; GO:0001764; P:neuron migration; ISO:RGD.
DR GO; GO:0042476; P:odontogenesis; ISO:RGD.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IEP:RGD.
DR GO; GO:0021983; P:pituitary gland development; ISO:RGD.
DR GO; GO:0043388; P:positive regulation of DNA binding; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0003350; P:pulmonary myocardium development; ISO:RGD.
DR GO; GO:0060577; P:pulmonary vein morphogenesis; ISO:RGD.
DR GO; GO:0030334; P:regulation of cell migration; ISO:RGD.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0009725; P:response to hormone; IEP:RGD.
DR GO; GO:0033189; P:response to vitamin A; IEP:RGD.
DR GO; GO:0007519; P:skeletal muscle tissue development; ISO:RGD.
DR GO; GO:0048536; P:spleen development; ISO:RGD.
DR GO; GO:0021763; P:subthalamic nucleus development; ISO:RGD.
DR GO; GO:0060578; P:superior vena cava morphogenesis; ISO:RGD.
DR GO; GO:0035886; P:vascular associated smooth muscle cell differentiation; ISO:RGD.
DR GO; GO:0001570; P:vasculogenesis; ISO:RGD.
DR GO; GO:0055015; P:ventricular cardiac muscle cell development; ISO:RGD.
DR GO; GO:0060412; P:ventricular septum morphogenesis; ISO:RGD.
DR GO; GO:0016055; P:Wnt signaling pathway; ISO:RGD.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..324
FT /note="Pituitary homeobox 2"
FT /id="PRO_0000049225"
FT DNA_BIND 92..151
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 57..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 286..299
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 292..296
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 57..90
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 97
FT /note="Phosphothreonine; by PKB/AKT2"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..53
FT /note="Missing (in isoform PTX2B)"
FT /evidence="ECO:0000303|PubMed:11032870,
FT ECO:0000303|PubMed:9748586"
FT /id="VSP_002264"
FT VAR_SEQ 54..68
FT /note="LEVHTISDTSSPEVA -> METNCRKLVSACVQL (in isoform
FT PTX2B)"
FT /evidence="ECO:0000303|PubMed:11032870,
FT ECO:0000303|PubMed:9748586"
FT /id="VSP_002265"
SQ SEQUENCE 324 AA; 35758 MW; 0FAB2ABA8CF180E3 CRC64;
MNCMKGPLPL EHRAAGTKLS AASSPFCHHT QALAMASVLA PGQPRSLDAS KHRLEVHTIS
DTSSPEVAEK DKGQQGKNED VGAEDPSKKK RQRRQRTHFT SQQLQELEAT FQRNRYPDMS
TREEIAVWTN LTEARVRVWF KNRRAKWRKR ERNQQAELCK NGFGPQFNGL MQPYDDMYPG
YSYNNWAAKG LTSASLSTKS FPFFNSMNVN PLSSQSMFSP PNSISSMSMS SSMVPSAVTG
VPGSSLNSLN NLNNLSSPSL NSAVPTPACP YAPPTPPYVY RDTCNSSLAS LRLKAKQHSS
FGYASVQNPA SNLSACQYAV DRPV
