PITX2_XENLA
ID PITX2_XENLA Reviewed; 326 AA.
AC Q9PWR3; O93396; Q9YHA4;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Pituitary homeobox 2;
DE AltName: Full=Homeobox protein PITX2;
DE AltName: Full=Paired-like homeodomain transcription factor 2;
DE AltName: Full=xPtx2;
GN Name=pitx2; Synonyms=ptx2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PTX2A).
RX PubMed=9707115; DOI=10.1038/29004;
RA Ryan A.K., Blumberg B., Rodriguez-Esteban C., Yonei-Tamura S., Tamura K.,
RA Tsukui T., de la Pena J., Sabbagh W., Greenwald J., Choe S., Norris D.P.,
RA Robertson E.J., Evans R.M., Rosenfeld M.G., Izpisua-Belmonte J.-C.;
RT "Pitx2 determines left-right asymmetry of internal organs in vertebrates.";
RL Nature 394:545-551(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PTX2A AND PTX2B).
RC TISSUE=Embryo;
RX PubMed=10021341; DOI=10.1242/dev.126.6.1225;
RA Campione M., Steinbeisser H., Schweickert A., Deissler K., van Bebber F.,
RA Lowe L.A., Nowotschin S., Viebahn C., Haffter P., Kuehn M.R., Blum M.;
RT "The homeobox gene Pitx2: mediator of asymmetric left-right signaling in
RT vertebrate heart and gut looping.";
RL Development 126:1225-1234(1999).
RN [3]
RP INDUCTION.
RX PubMed=16959238; DOI=10.1016/j.ydbio.2006.08.021;
RA Ohi Y., Wright C.V.E.;
RT "Anteriorward shifting of asymmetric Xnr1 expression and contralateral
RT communication in left-right specification in Xenopus.";
RL Dev. Biol. 301:447-463(2007).
CC -!- FUNCTION: May play an important role in development and maintenance of
CC anterior structures and mediate left-right asymmetry. Could play a role
CC at the interface of lateral plate signaling and heart and gut
CC morphogenesis.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=PTX2A;
CC IsoId=Q9PWR3-1; Sequence=Displayed;
CC Name=PTX2B;
CC IsoId=Q9PWR3-2; Sequence=VSP_002270;
CC -!- DEVELOPMENTAL STAGE: Asymmetrically expressed in the left lateral plate
CC mesoderm, tubular heart and early gut tube.
CC -!- INDUCTION: By nodal/nr-1 in the left lateral plate mesoderm.
CC {ECO:0000269|PubMed:16959238}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AF077767; AAC29426.1; -; mRNA.
DR EMBL; AJ005786; CAA06696.1; -; mRNA.
DR EMBL; AJ005787; CAA06697.1; -; mRNA.
DR RefSeq; NP_001079055.1; NM_001085586.1.
DR RefSeq; NP_001081756.1; NM_001088287.1.
DR AlphaFoldDB; Q9PWR3; -.
DR SMR; Q9PWR3; -.
DR DNASU; 373586; -.
DR GeneID; 373586; -.
DR GeneID; 398033; -.
DR KEGG; xla:373586; -.
DR KEGG; xla:398033; -.
DR CTD; 373586; -.
DR CTD; 398033; -.
DR Xenbase; XB-GENE-482496; pitx2.L.
DR Xenbase; XB-GENE-6253059; pitx2.S.
DR OMA; CPYATAP; -.
DR OrthoDB; 1432356at2759; -.
DR Proteomes; UP000186698; Chromosome 1L.
DR Proteomes; UP000186698; Chromosome 1S.
DR Bgee; 373586; Expressed in muscle tissue and 7 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0048562; P:embryonic organ morphogenesis; TAS:AgBase.
DR GO; GO:0003007; P:heart morphogenesis; TAS:AgBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome.
FT CHAIN 1..326
FT /note="Pituitary homeobox 2"
FT /id="PRO_0000049228"
FT DNA_BIND 94..153
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 17..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 288..301
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 294..298
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 66..92
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..70
FT /note="MNSMKEPLHLDHLAGSRVAAASSHHHHHHHHQHQTVTLVSMASSLGQRSGEC
FT KSRLEVHTISDTSSPDTA -> MDSNCRKLVTTCVQLGVQPSAVECLFSKESDMKKGGF
FT SAADGTEGNRKETGKRFSRIHQSGS (in isoform PTX2B)"
FT /evidence="ECO:0000303|PubMed:10021341"
FT /id="VSP_002270"
FT CONFLICT 78
FT /note="T -> S (in Ref. 2; CAA06697)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="S -> N (in Ref. 2; CAA06697)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="A -> T (in Ref. 2; CAA06697)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="A -> T (in Ref. 2; CAA06697)"
FT /evidence="ECO:0000305"
FT CONFLICT 306..307
FT /note="Missing (in Ref. 2; CAA06697)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="T -> N (in Ref. 2; CAA06697)"
FT /evidence="ECO:0000305"
FT CONFLICT 323
FT /note="D -> N (in Ref. 1; AAC29426)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 326 AA; 36339 MW; 74366F0E4FB48E73 CRC64;
MNSMKEPLHL DHLAGSRVAA ASSHHHHHHH HQHQTVTLVS MASSLGQRSG ECKSRLEVHT
ISDTSSPDTA DKDKSHQTKN EDSSTDDPSK KKRQRRQRTH FTSQQLQELE ATFQRNRYPD
MSTREEIAVW TNLTEARVRV WFKNRRAKWR KRERNQQAEL CKNGFGPQFN GLMQPYDDMY
PSYSYNNWAA KGLTSASLST KSFPFFNSMN VNPLSSQSMF SSPNSISSMS MSSGMVPSAV
TGVPGSGLNS LNNLNNLSNP SLNTAVPTSA CPYAPPTPPY VYRDTCNSSL ASLRLKAKQH
SSFGYATVQT PGSNLSACQY AVDRPV
