ID   PITX_BRABE              Reviewed;         331 AA.
AC   Q9U637;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Pituitary homeobox x {ECO:0000250|UniProtKB:Q9W5Z2};
DE   AltName: Full=Bicoid type transcription factor Pitx {ECO:0000312|EMBL:AAF03901.1};
DE            Short=BbPtx {ECO:0000303|PubMed:10654612};
DE   AltName: Full=Homeobox protein Ptx {ECO:0000250|UniProtKB:Q9W5Z2};
DE   AltName: Full=Paired-like homeodomain transcription factor x {ECO:0000250|UniProtKB:Q9W5Z2};
GN   Name=Ptx {ECO:0000312|EMBL:AAF03901.1};
OS   Branchiostoma belcheri (Amphioxus).
OC   Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC   Branchiostomidae; Branchiostoma.
OX   NCBI_TaxID=7741;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAF03901.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=10654612; DOI=10.1242/dev.127.1.187;
RA   Yasui K., Zhang S., Uemura M., Saiga H.;
RT   "Left-right asymmetric expression of BbPtx, a Ptx-related gene, in a
RT   lancelet species and the developmental left-sidedness in deuterostomes.";
RL   Development 127:187-195(2000).
CC   -!- FUNCTION: Involved in left-right asymmetry in the developing embryo.
CC       {ECO:0000269|PubMed:10654612}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00138}.
CC   -!- DEVELOPMENTAL STAGE: Expression is first detected in the early mid-
CC       gastrulae at the dorsal margin of the blastospore and at the anterior
CC       region of the ectoderm until the late gastrula stage. It then becomes
CC       restricted to the left anterodorsal wall of the primitive gut and to
CC       the developing left somitocoelomic system in the early neurulae stage.
CC       Expression continues on the left side in the developing preoral pit,
CC       the duct of the club-shaped gland and mouth as well as in the mesoderm
CC       at the caudal end. No expression is seen in the gland body on the right
CC       side or around the gill pores. By the mid-neurulae stage expression is
CC       detected in some cells of the central nervous system (CNS), this
CC       continues along with posterior expression in to the early larval stage.
CC       Around the rim of the mouth, expression is detected in the endoderm and
CC       ectoderm at the 36 and 48 hour larval stages. Left-sided asymmetric
CC       expression continues into the 48 hour larval stage in the preoral pit,
CC       but becomes weak or disappears in other regions.
CC       {ECO:0000269|PubMed:10654612}.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC       {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF195616; AAF03901.1; -; mRNA.
DR   AlphaFoldDB; Q9U637; -.
DR   SMR; Q9U637; -.
DR   Proteomes; UP000515135; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR016233; Homeobox_Pitx/unc30.
DR   InterPro; IPR003654; OAR_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF03826; OAR; 1.
DR   PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS50803; OAR; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..331
FT                   /note="Pituitary homeobox x"
FT                   /id="PRO_0000396654"
FT   DNA_BIND        105..164
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          25..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          53..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           289..302
FT                   /note="OAR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT   MOTIF           295..299
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q9W5Z2, ECO:0000255"
FT   COMPBIAS        53..92
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   331 AA;  35780 MW;  2661AC42286086DB CRC64;
     MDTLNDSLSL EQLVSVSPRR SQLTMAGIPP INNSGTSTGS ALDSTAVSGA HTSLAGTDSS
     MDSTHGSGAA TVSAGSPVGK DSGSGSTPST DVTQDDDEMR KRRRQRRQRT HFTSQQLQEL
     EASFARNRYP DMATREEIAA WTNLTEARVR VWFKNRRAKW RKRERNQLGE FKNGFGPHFN
     GLMQPFDDGL YSGYSPAYNN WAAKVPSPLT AKSFPWGLNS SGVPNVNPLS SQAMCFTPPT
     TIGTATTMVP SMNVGNGLNS LSSLQNPTVA PCPYASPGQP YAYREQCNSS IAALRLKAKQ
     HSTSVASSFS YPSPVRQQTL SACQYAVDRP V