PITX_DROME
ID PITX_DROME Reviewed; 509 AA.
AC O18400; Q9VA26;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Pituitary homeobox homolog Ptx1;
DE Short=D-PTX1;
GN Name=Ptx1; ORFNames=CG1447;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Embryo;
RX PubMed=9431811; DOI=10.1016/s0925-4773(97)00139-1;
RA Vorbrueggen G., Constien R., Zilian O., Wimmer E.A., Dowe G., Taubert H.,
RA Noll M., Jaeckle H.;
RT "Embryonic expression and characterization of a Ptx1 homolog in
RT Drosophila.";
RL Mech. Dev. 68:139-147(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Appears to control physiological cell functions rather than
CC pattern formation during embryogenesis. {ECO:0000269|PubMed:9431811}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00138, ECO:0000269|PubMed:9431811}.
CC -!- DEVELOPMENTAL STAGE: First detected in the posterior region of the
CC blastoderm embryo. In later stages of embryonic development, detected
CC in the posterior portion of the midgut, in the developing malpighian
CC tubules, in a subset of ventral somatic muscles, in the developing CNS
CC and in Bolwig's organ. {ECO:0000269|PubMed:9431811}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ001519; CAA04801.1; -; mRNA.
DR EMBL; AE014297; AAF57099.3; -; Genomic_DNA.
DR EMBL; BT009990; AAQ22459.1; -; mRNA.
DR RefSeq; NP_733410.2; NM_170531.4.
DR AlphaFoldDB; O18400; -.
DR SMR; O18400; -.
DR BioGRID; 68514; 26.
DR IntAct; O18400; 23.
DR STRING; 7227.FBpp0271894; -.
DR PaxDb; O18400; -.
DR DNASU; 43664; -.
DR EnsemblMetazoa; FBtr0089389; FBpp0088412; FBgn0020912.
DR GeneID; 43664; -.
DR KEGG; dme:Dmel_CG1447; -.
DR UCSC; CG1447-RA; d. melanogaster.
DR CTD; 43664; -.
DR FlyBase; FBgn0020912; Ptx1.
DR VEuPathDB; VectorBase:FBgn0020912; -.
DR eggNOG; KOG0486; Eukaryota.
DR GeneTree; ENSGT00940000168132; -.
DR HOGENOM; CLU_030301_3_1_1; -.
DR InParanoid; O18400; -.
DR OMA; ANHQDEG; -.
DR PhylomeDB; O18400; -.
DR BioGRID-ORCS; 43664; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Ptx1; fly.
DR GenomeRNAi; 43664; -.
DR PRO; PR:O18400; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0020912; Expressed in embryonic/larval Malpighian tubule (Drosophila) and 39 other tissues.
DR ExpressionAtlas; O18400; baseline and differential.
DR Genevisible; O18400; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005667; C:transcription regulator complex; ISS:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:FlyBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..509
FT /note="Pituitary homeobox homolog Ptx1"
FT /id="PRO_0000049233"
FT DNA_BIND 262..322
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 70..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 460..473
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 464..470
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 70..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 9..49
FT /note="GCGGGGGLGVGVVTSSATALGPGGLTNGGGGVVSGALNGLE -> LRRGRRS
FT GSGRGHQLGHGLGTGWPDQRGRRSGQWRTQRIG (in Ref. 1; CAA04801)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="T -> A (in Ref. 1; CAA04801)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="I -> SLFFSV (in Ref. 1; CAA04801)"
FT /evidence="ECO:0000305"
FT CONFLICT 283
FT /note="F -> S (in Ref. 1; CAA04801)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 509 AA; 53217 MW; 6C01616DB9D79C88 CRC64;
MDRSSAVGGC GGGGGLGVGV VTSSATALGP GGLTNGGGGV VSGALNGLEA MSAESTGLCL
QDLVSAGTAN GAGSAGSAES ATTTSTALSS GSTGSSTVNG GGSSTSGTEH LHSHHSLHDS
SSSVSISPAI SSLMPISSLS HLHHSAGQDL VGGYSQHPHH TVVPPHTPKH EPLEKLRIWA
ETGDFRDSHS SMTAVANSLD STHLNNFQTS STSSISNRSR DRKDGNRSVN ETTIKTENIS
SSGHDEPMTT SGEEPKNDKK NKRQRRQRTH FTSQQLQELE HTFSRNRYPD MSTREEIAMW
TNLTEARVRV WFKNRRAKWR KRERNAMNAA VAAADFKSGF GTQFMQPFAD DSLYSSYPYN
NWTKVPSPLG TKPFPWPVNP LGSMVAGNHH QNSVNCFNTG ASGVAVSMNN ASMLPGSMGS
SLSNTSNVGA VGAPCPYTTP ANPYMYRSAA EPCMSSSMSS SIATLRLKAK QHASAGFGSP
YSAPSPVSRS NSAGLSACQY TGVGVTDVV
