ID   PIT_MYCTU               Reviewed;         417 AA.
AC   P9WIA7; L0T6T9; O06411;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Probable low-affinity inorganic phosphate transporter;
GN   Name=pit; Synonyms=pitA; OrderedLocusNames=Rv0545c; ORFNames=MTCY25D10.24c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
CC   -!- FUNCTION: Low-affinity inorganic phosphate transport. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC       2.A.20) family. Pit subfamily. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43283.1; -; Genomic_DNA.
DR   PIR; D70547; D70547.
DR   RefSeq; NP_215059.1; NC_000962.3.
DR   RefSeq; WP_003911224.1; NZ_NVQJ01000036.1.
DR   AlphaFoldDB; P9WIA7; -.
DR   SMR; P9WIA7; -.
DR   STRING; 83332.Rv0545c; -.
DR   PaxDb; P9WIA7; -.
DR   DNASU; 887517; -.
DR   GeneID; 887517; -.
DR   KEGG; mtu:Rv0545c; -.
DR   TubercuList; Rv0545c; -.
DR   eggNOG; COG0306; Bacteria.
DR   OMA; GYDITRT; -.
DR   PhylomeDB; P9WIA7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0035435; P:phosphate ion transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR001204; Phos_transporter.
DR   PANTHER; PTHR11101; PTHR11101; 1.
DR   Pfam; PF01384; PHO4; 2.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Phosphate transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..417
FT                   /note="Probable low-affinity inorganic phosphate
FT                   transporter"
FT                   /id="PRO_0000080792"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          379..417
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        388..405
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   417 AA;  42745 MW;  427A06C3237F7574 CRC64;
     MNLQLFLLLI VVVTALAFDF TNGFHDTGNA MATSIASGAL APRVAVALPA VLNLIGAFLS
     TAVAATIAKG LIDANLVTLE LVFAGLVGGI VWNLLTWLLG IPSSSSHALI GGIVGATIAA
     VGLRGVIWSG VVSKVIVPAV VAALLATLVG AVGTWLVYRT TRGVAEKRTE RGFRRGQIGS
     ASLVSLAHGT NDAQKTMGVI FLALMSYGAV STTASVPPLW VIVSCAVAMA AGTYLGGWRI
     IRTLGKGLVE IKPPQGMAAE SSSAAVILLS AHFGYALSTT QVATGSVLGS GVGKPGAEVR
     WGVAGRMVVA WLVTLPLAGL VGAFTYGLVH FIGGYPGAIL GFALLWLTAT AIWLRSRRAP
     IDHTNVNADW EGNLTAGLEA GAQPLADQRP PVPAPPAPTP PPNHRAPQFG VTTRNAP