PIUC_PSEAE
ID PIUC_PSEAE Reviewed; 226 AA.
AC Q9HVQ7; O68589;
DT 20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=PKHD-type hydroxylase PiuC;
DE EC=1.14.11.-;
DE AltName: Full=Iron-uptake factor PiuC;
GN Name=piuC; OrderedLocusNames=PA4515;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RA Ochsner U.A., Vasil A.I., Johnson Z., Vasil M.L.;
RT "Genetic characterization of novel siderophore receptor genes involved in
RT iron acquisition in Pseudomonas aeruginosa.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290; Evidence={ECO:0000250};
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DR EMBL; AF051690; AAC06214.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG07903.1; -; Genomic_DNA.
DR PIR; E83081; E83081.
DR RefSeq; NP_253205.1; NC_002516.2.
DR RefSeq; WP_003112849.1; NZ_QZGE01000004.1.
DR AlphaFoldDB; Q9HVQ7; -.
DR SMR; Q9HVQ7; -.
DR STRING; 287.DR97_1694; -.
DR PaxDb; Q9HVQ7; -.
DR DNASU; 881121; -.
DR EnsemblBacteria; AAG07903; AAG07903; PA4515.
DR GeneID; 881121; -.
DR KEGG; pae:PA4515; -.
DR PATRIC; fig|208964.12.peg.4725; -.
DR PseudoCAP; PA4515; -.
DR HOGENOM; CLU_106663_0_0_6; -.
DR InParanoid; Q9HVQ7; -.
DR OMA; FPPLFNC; -.
DR PhylomeDB; Q9HVQ7; -.
DR BioCyc; PAER208964:G1FZ6-4604-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016706; F:2-oxoglutarate-dependent dioxygenase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IBA:GO_Central.
DR GO; GO:0071281; P:cellular response to iron ion; EXP:CollecTF.
DR HAMAP; MF_00657; Hydroxyl_YbiX; 1.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR041097; PKHD_C.
DR InterPro; IPR023550; PKHD_hydroxylase.
DR InterPro; IPR006620; Pro_4_hyd_alph.
DR InterPro; IPR044862; Pro_4_hyd_alph_FE2OG_OXY.
DR PANTHER; PTHR41536; PTHR41536; 1.
DR Pfam; PF13640; 2OG-FeII_Oxy_3; 1.
DR Pfam; PF18331; PKHD_C; 1.
DR SMART; SM00702; P4Hc; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome;
KW Vitamin C.
FT CHAIN 1..226
FT /note="PKHD-type hydroxylase PiuC"
FT /id="PRO_0000206680"
FT DOMAIN 78..178
FT /note="Fe2OG dioxygenase"
FT BINDING 96
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 98
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 159
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 169
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000255"
FT CONFLICT 216
FT /note="Y -> I (in Ref. 1; AAC06214)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 226 AA; 25616 MW; 17F711DBDC5FEBAA CRC64;
MLLHIPAIFT AEEVSRIRAA LEQAEWADGK ATAGYQSAKA KHNLQLPQDH PLAREIGEAM
LQRLWNHPLF MSAALPLKVF PPLFNCYTGG GSFDFHIDNA VRDVHGGRER VRTDLSSTLF
FSDPEDYDGG ELVIQDTYGL QQVKLPAGDL VLYPGTSLHK VNPVTRGARY ASFFWTQSLV
REDSQRTLLF EMDQSIQRLT RDVPDHPSLI RLTGTYHNLL RRWSEL
