PIYH1_XYLFT
ID PIYH1_XYLFT Reviewed; 1472 AA.
AC Q87B49;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Type IV pilus biogenesis factor PilY1 homolog PD_1611 {ECO:0000250|UniProtKB:S0HPF7, ECO:0000303|PubMed:25217013};
GN Name=pilY1_1 {ECO:0000312|EMBL:AAO29451.1};
GN OrderedLocusNames=PD_1611 {ECO:0000312|EMBL:AAO29451.1};
OS Xylella fastidiosa (strain Temecula1 / ATCC 700964).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xylella.
OX NCBI_TaxID=183190 {ECO:0000312|EMBL:AAO29451.1};
RN [1] {ECO:0000312|EMBL:AAO29451.1, ECO:0000312|Proteomes:UP000002516}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Temecula1 / ATCC 700964 {ECO:0000312|Proteomes:UP000002516};
RX PubMed=12533478; DOI=10.1128/jb.185.3.1018-1026.2003;
RA Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y.,
RA Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A.,
RA Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S.,
RA Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M., Carrer H.,
RA Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L.,
RA Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L.,
RA Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S.,
RA Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F.,
RA Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G.,
RA Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A.,
RA Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L.,
RA Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P.;
RT "Comparative analyses of the complete genome sequences of Pierce's disease
RT and citrus variegated chlorosis strains of Xylella fastidiosa.";
RL J. Bacteriol. 185:1018-1026(2003).
RN [2]
RP FUNCTION, CALCIUM-BINDING, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Temecula1 / ATCC 700964 {ECO:0000303|PubMed:25217013};
RX PubMed=25217013; DOI=10.1128/aem.02153-14;
RA Cruz L.F., Parker J.K., Cobine P.A., De La Fuente L.;
RT "Calcium-enhanced twitching motility in Xylella fastidiosa is linked to a
RT single PilY1 homolog.";
RL Appl. Environ. Microbiol. 80:7176-7185(2014).
CC -!- FUNCTION: One of the three PilY1 homologs of X.fastidiosa, which are
CC involved in bacterial twitching motility as component of the
CC filamentous type IV pili (T4P). The twitching motility of this protein
CC is enhanced by calcium, which may provide the bacterium an adaptive
CC advantage in environments with high calcium concentrations.
CC {ECO:0000269|PubMed:25217013, ECO:0000305|PubMed:25217013}.
CC -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000305|PubMed:25217013}.
CC -!- INDUCTION: Expression is not affected by calcium.
CC {ECO:0000269|PubMed:25217013}.
CC -!- DISRUPTION PHENOTYPE: Shows twitching motility. Loss of increased
CC twitching motility at higher Ca(2+) concentrations. Forms T4P. Impaired
CC in the calcium-mediated increase in single-cell attachment to surfaces,
CC but biofilm formation is not affected. {ECO:0000269|PubMed:25217013}.
CC -!- SIMILARITY: Belongs to the PilY1 family. {ECO:0000305}.
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DR EMBL; AE009442; AAO29451.1; -; Genomic_DNA.
DR AlphaFoldDB; Q87B49; -.
DR SMR; Q87B49; -.
DR EnsemblBacteria; AAO29451; AAO29451; PD_1611.
DR KEGG; xft:PD_1611; -.
DR HOGENOM; CLU_001890_0_1_6; -.
DR OMA; DPLWYAA; -.
DR Proteomes; UP000002516; Chromosome.
DR GO; GO:0044096; C:type IV pilus; IC:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043107; P:type IV pilus-dependent motility; IMP:UniProtKB.
DR InterPro; IPR008707; PilC_beta_prop_dom.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR Pfam; PF05567; Neisseria_PilC; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
PE 1: Evidence at protein level;
KW Calcium; Fimbrium; Fimbrium biogenesis; Metal-binding.
FT CHAIN 1..1472
FT /note="Type IV pilus biogenesis factor PilY1 homolog
FT PD_1611"
FT /id="PRO_0000431918"
FT REGION 1383..1403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 1172
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 1174
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 1176
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 1178
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
SQ SEQUENCE 1472 AA; 158643 MW; 5CD0D5481BC04ADA CRC64;
MLVMGRDHKL YYEAYNDASD LDGDGVLDVG YKPDKITYYG YYNSNVCYRA NGTMFQAMSV
ANGSNGKKCT GAWSGDFLNY LTTSRMDALR KVLFGGYREV DTLTQTILRA SYTPQDAHSW
GKEYASVSHD GYDISDYAPL SAPGSGHYHL FAVTTLSDNG IPQLRVLADT TFRVWNWVSI
ERPVAGSDCF TPENKRVSCV SGGSRGISDY PLRVEVCSVA DELRESNCKL YQNNTSYKPT
GILHDYGEND RMYFGLLTGS YQKNITGGVL HSNVSNFSRE INPLTGQFCL NGNCGGGGDV
KGIVHTISSF RMLDFNYKDY TYGCGWIATR PVKEGECWMW GNPVAEMMYE TLRYFGGATA
PRPEYDINSS SQDIATLQLS HPGWKPPYTS VDKGGSGYSV CAQPTMTVFS DINPSYDDKL
PGSHWSNFSG SGDPASMRNL DVSAEADRIW EAEGGGSKLF FIGESNNNSD NAPTPKVVSN
LSTVRGLSPE EPSKGGTYYS AAVARYGANH KMGGSKFVRT YAVALASPLP KFEFPVGNAR
VSLVPFAKSV RGFGISATGN FQPTNQIIDF YVQRVANMAG SSGADYDATI NGGRPYAEFR
INYEDVEQGA DHDMDAIALY TIYVNAKNQL VVTLKSEYSA GSIDQHMGYV ISGTTRDGVY
LEICDLADGH SNDGTRSSCA GQQPYKLNTP PNRLPGYCNA TPMPGDCNGL PPVATRIFSV
ASQGANAVLL KDPLWYAAKY GHDQGVILNS SGGLANYFPV NNALYLRQQV AKAFSAIQSQ
AGSSGSIAVV GASVSSTSFA VIPSYSSTHD GKDWTGEMTA YRIDANGMIG DVLWLASAGV
PSGTSAIAKR VIYTALSHVD DTNRASVVRR FVAEKLVDSS SGDVATDAAQ VFGRLGYTPR
GVIDDFGSSV TPNQLVNYLR GDKRMEGATL NTAPFRRRFG PLGDMINSIP VVATRRANYG
WATASGLPQV QRDSYSAFIN ARQNSTAAEH IFVGANDGML HAFDDKGTER FAYVPNGVLH
HLGFLANPEY QHHYYVDGKS TLSDAYLDGS WRSVLVGGTG AGGRSMFALD VTSPSTFNES
NVLWEMNSEN DDDMGYTMGK PYIVPLQNGS WAAIFGNGYN STNGRAVLFI VNLATGQLIR
KIEARDGIDP DGSDPANMGY NGLGNLAVLD TDGDGLVDMV YGADLHGNLW KFNLSGKDPQ
RWGIAYKDGL GNPIPLFVAR NPQGYRQPIT GGLEVAVGPS AGYIIYFGSG RYFAANDNNS
KDLSTLYGIW DSGSPVIAGR AALSAQIIQA SDHPTSPDTR IVTRRPLSYL SKHGWYVDLV
VQGQDPQGER SIATPLLQGG RVFFSTYVPG VSVNCASGGS NWLYVLDAAS GGAALGQVNI
PSRGSRSSIG NSDTGAVSTG GDAPIQSVAM TRTAPRQPVF CNPGEQGCPL VPETHAAPLD
TRCSEVIIDP NDPTRSISLF RACGRQSWRQ LR
