PIYH3_XYLFT
ID PIYH3_XYLFT Reviewed; 1214 AA.
AC Q87FA5;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Type IV pilus biogenesis factor PilY1 homolog PD_0023 {ECO:0000250|UniProtKB:S0HPF7, ECO:0000303|PubMed:25217013};
DE Flags: Precursor;
GN Name=pilY1_3 {ECO:0000312|EMBL:AAO27930.1};
GN OrderedLocusNames=PD_0023 {ECO:0000312|EMBL:AAO27930.1};
OS Xylella fastidiosa (strain Temecula1 / ATCC 700964).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xylella.
OX NCBI_TaxID=183190 {ECO:0000312|EMBL:AAO27930.1};
RN [1] {ECO:0000312|EMBL:AAO27930.1, ECO:0000312|Proteomes:UP000002516}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Temecula1 / ATCC 700964 {ECO:0000312|Proteomes:UP000002516};
RX PubMed=12533478; DOI=10.1128/jb.185.3.1018-1026.2003;
RA Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y.,
RA Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A.,
RA Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S.,
RA Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M., Carrer H.,
RA Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L.,
RA Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L.,
RA Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S.,
RA Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F.,
RA Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G.,
RA Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A.,
RA Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L.,
RA Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P.;
RT "Comparative analyses of the complete genome sequences of Pierce's disease
RT and citrus variegated chlorosis strains of Xylella fastidiosa.";
RL J. Bacteriol. 185:1018-1026(2003).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Temecula1 / ATCC 700964 {ECO:0000303|PubMed:17693510};
RX PubMed=17693510; DOI=10.1128/jb.00934-07;
RA De La Fuente L., Burr T.J., Hoch H.C.;
RT "Mutations in type I and type IV pilus biosynthetic genes affect twitching
RT motility rates in Xylella fastidiosa.";
RL J. Bacteriol. 189:7507-7510(2007).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=Temecula1 / ATCC 700964 {ECO:0000303|PubMed:17322192};
RX PubMed=17322192; DOI=10.1099/mic.0.2006/002311-0;
RA Li Y., Hao G., Galvani C.D., Meng Y., De La Fuente L., Hoch H.C.,
RA Burr T.J.;
RT "Type I and type IV pili of Xylella fastidiosa affect twitching motility,
RT biofilm formation and cell-cell aggregation.";
RL Microbiology 153:719-726(2007).
RN [4]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Temecula1 / ATCC 700964 {ECO:0000303|PubMed:25217013};
RX PubMed=25217013; DOI=10.1128/aem.02153-14;
RA Cruz L.F., Parker J.K., Cobine P.A., De La Fuente L.;
RT "Calcium-enhanced twitching motility in Xylella fastidiosa is linked to a
RT single PilY1 homolog.";
RL Appl. Environ. Microbiol. 80:7176-7185(2014).
CC -!- FUNCTION: One of the three PilY1 homologs of X.fastidiosa, which are
CC involved in bacterial twitching motility as component of the
CC filamentous type IV pili (T4P). {ECO:0000269|PubMed:17693510,
CC ECO:0000305|PubMed:25217013}.
CC -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000250|UniProtKB:Q87B49}.
CC -!- INDUCTION: Expression is not affected by calcium.
CC {ECO:0000269|PubMed:25217013}.
CC -!- DISRUPTION PHENOTYPE: Shows twitching motility, but the speed of the
CC twitching movement is three times slower than wild-type on a glass
CC surface against the flow direction of media. Mutant cells also move
CC more sporadically with frequent stop intervals. Forms T4P
CC (PubMed:17693510,PubMed:17322192). Not impaired in attachment to
CC surfaces or biofilm formation (PubMed:25217013).
CC {ECO:0000269|PubMed:17322192, ECO:0000269|PubMed:17693510,
CC ECO:0000269|PubMed:25217013}.
CC -!- SIMILARITY: Belongs to the PilY1 family. {ECO:0000305}.
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DR EMBL; AE009442; AAO27930.1; -; Genomic_DNA.
DR RefSeq; WP_011097477.1; NC_004556.1.
DR AlphaFoldDB; Q87FA5; -.
DR SMR; Q87FA5; -.
DR EnsemblBacteria; AAO27930; AAO27930; PD_0023.
DR GeneID; 58015588; -.
DR KEGG; xft:PD_0023; -.
DR HOGENOM; CLU_001890_0_1_6; -.
DR OMA; IDPFRWA; -.
DR Proteomes; UP000002516; Chromosome.
DR GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR008707; PilC_beta_prop_dom.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR Pfam; PF05567; Neisseria_PilC; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
PE 2: Evidence at transcript level;
KW Calcium; Fimbrium; Fimbrium biogenesis; Metal-binding; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..1214
FT /note="Type IV pilus biogenesis factor PilY1 homolog
FT PD_0023"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431920"
FT REGION 307..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 438..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..458
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 952
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 954
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 956
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
FT BINDING 958
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:S0HPF7"
SQ SEQUENCE 1214 AA; 132880 MW; E1E8913521F98C3A CRC64;
MKKTVFNPAL NRAAAILIGT LVGISGVVHA SVDISSSPLH GGKDVPGNLA ILASVEFPTL
ISVANLADTY TPGVRYVGYF DSNKCYKYHY SSRELDRYFY PVASPRPQAN YGCNTTGGVW
AGNFLNWAAT QTIDPFRSAL TGGYRVRDTV SETILEKAVM DRDSQENFPR RNVAGRNVLA
TLVPTQWNNF RIRIDGLGNR MRFTQFSSLW TDPLNTEGQP YDSSRHPLNR NDRGVYEVSV
RVKVCDPSAG LESNCVAYPR GSYKPEGLIQ EYSKRIRYSV FGYKNDHSYL IDGGVLRARQ
KFVGPQTHYP EQGKKTNSHA EWDPQTGILY DNPDPEDAAA TTRRVGRTIA NSGVINYLNK
SGQMDTGRIS KIYDPVSELY YTAYRYFKRL GNVPEYSVLT GSVNEKYQQA DAFPVITDWD
DPIRYACQSN VVLGIGDTHT NQDKNLPGNT NTMEEPSKPQ TVRNDRSIDV VKRMAQIFRM
EGMRQQDAMT SAVAPKFNFH RYNSAYIAAL AYDAHTKDMR PDLEGDQLLT THWVDVVEAG
DYKIPISTNQ YWLAAKYGGF QVPAGYDPDK TVNPLSEATW WTNGEYVNND LKANAKRADN
FYVAADAEKM VASLKHAFSR IVAEIKGAGT GLSSNSARLE TGAVTYQAQF FSGTWRGDLI
AYHVDKVTGA LTPFWNANFP AWEQRVIKFA NATTLQDFTK KNLGQTALAS ASAQQINYLR
GDRSQEGNVP GKLRIRSGIM GDIVNSQPLY VGAPNGRLYT TANFTGASAY AAFAAQQANR
VPVVYVGAND GMLHAFDANT GKEIFAFVPR AAMPKLLEYT DQNYGHQYYV DGELTAADIY
DTKLGWRSVL VGTLGRGGKG LFALDVTDPS NIRLLWDKTS ADIGGLGNTL SKPMIAQTSD
GTWSVLLGNG PNSTADNAQL IVMNLLTGHA AQVPVSKTSN NGLSGVFPWS SQSNGITDRV
YAGDLLGTLW RFTFSDNAWK VAPLFTATYQ GKAQPISATP LGAIERSTGR MWIFFGTGRA
LSSHDMDNKE VQSWYGLIDQ GTTIPGRTRL SQVQIVDEGV VNGYAVRTVS DPKNIGTDGW
YMDLISPKSG KQGERMIVSN MFRGAALIGT TRIPDNSDIC KLSGSGFVMA INPFTGGRLG
QWFFDLNTGG GSGGALNGNP VSGVGVSSAP NSPVFTGNIM QIGADDGTVT SLKTPSSGGL
NINRVSWREI LRTE
