ID   PK22_PSESM              Reviewed;         498 AA.
AC   Q886D9;
DT   20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Polyphosphate:AMP phosphotransferase {ECO:0000305};
DE            Short=PAP {ECO:0000250|UniProtKB:Q83XD3};
DE            EC=2.7.4.33 {ECO:0000269|PubMed:19001261};
DE   AltName: Full=Polyphosphate kinase PPK2 {ECO:0000305};
GN   OrderedLocusNames=PSPTO_1640 {ECO:0000312|EMBL:AAO55160.1};
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=19001261; DOI=10.1073/pnas.0807563105;
RA   Nocek B., Kochinyan S., Proudfoot M., Brown G., Evdokimova E., Osipiuk J.,
RA   Edwards A.M., Savchenko A., Joachimiak A., Yakunin A.F.;
RT   "Polyphosphate-dependent synthesis of ATP and ADP by the family-2
RT   polyphosphate kinases in bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:17730-17735(2008).
CC   -!- FUNCTION: Uses inorganic polyphosphate (polyP) as a donor to convert
CC       AMP to ADP. Can also convert GMP to GDP, with lower efficiency.
CC       {ECO:0000269|PubMed:19001261}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[phosphate](n) + ADP = [phosphate](n+1) + AMP;
CC         Xref=Rhea:RHEA:57820, Rhea:RHEA-COMP:9859, Rhea:RHEA-COMP:14280,
CC         ChEBI:CHEBI:16838, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216;
CC         EC=2.7.4.33; Evidence={ECO:0000269|PubMed:19001261};
CC   -!- SIMILARITY: Belongs to the polyphosphate kinase 2 (PPK2) family. Class
CC       II subfamily. {ECO:0000305}.
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DR   EMBL; AE016853; AAO55160.1; -; Genomic_DNA.
DR   RefSeq; NP_791465.1; NC_004578.1.
DR   RefSeq; WP_011103643.1; NC_004578.1.
DR   AlphaFoldDB; Q886D9; -.
DR   SMR; Q886D9; -.
DR   STRING; 223283.PSPTO_1640; -.
DR   EnsemblBacteria; AAO55160; AAO55160; PSPTO_1640.
DR   GeneID; 1183277; -.
DR   KEGG; pst:PSPTO_1640; -.
DR   PATRIC; fig|223283.9.peg.1664; -.
DR   eggNOG; COG2326; Bacteria.
DR   HOGENOM; CLU_033786_0_2_6; -.
DR   OMA; SPLDWQQ; -.
DR   OrthoDB; 953793at2; -.
DR   PhylomeDB; Q886D9; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043751; F:polyphosphate:AMP phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006797; P:polyphosphate metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022489; PolyP_AMP_Tfrase.
DR   InterPro; IPR022488; PPK2-related.
DR   Pfam; PF03976; PPK2; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR03708; poly_P_AMP_trns; 1.
PE   1: Evidence at protein level;
KW   Kinase; Reference proteome; Repeat; Transferase.
FT   CHAIN           1..498
FT                   /note="Polyphosphate:AMP phosphotransferase"
FT                   /id="PRO_0000442598"
FT   REGION          11..234
FT                   /note="PPK2 1"
FT                   /evidence="ECO:0000305"
FT   REGION          269..491
FT                   /note="PPK2 2"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   498 AA;  57955 MW;  334F148213842F45 CRC64;
     MFESAEIGHA IDDDTYEAAL PSLREALLEA QIDLHEQAKR QIIVLINGIE GAGKGETVKL
     LSEWMDPRLI EVRTFDQQTD EELAHPPVWR YWRQLPAKGR MGIFFGNWYS QMLQGRVHGQ
     YKDAVLDQAI SGAERLEKML CDEGALIFKF WFHLSKKQMK LRLKTLKDDP LHSWRISPLD
     WQQSKTYDKF VRFGERVLRR TSRDYAPWHV IEGVDANYRS LTVGRLLLEG MQAALNKVEP
     ESSALTIGPL AIHNNERTLL DSLDLSLHLS KEDYQHELIA EQARLSGNLR DKRMKSHALV
     AVFEGNDAAG KGGAIRRVAA ALDPRQYAIV PIAAPTQDER AQPYLWRFWR QIPARGKFTI
     FDRSWYGRVL VERVEGFCSE SDWKRAYAEI NDFEEQLTEA GVVVVKFWLA IDEQTQLERF
     QEREKIPFKR YKITEDDWRN RKKWPDYRQA VGDMVDRTST EIAPWTLIEA NDKRWARVKV
     LRTINEALEK AFARDKKK