ID   PK66_PLAKU              Reviewed;         563 AA.
AC   P21303;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 2.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Merozoite receptor PK66;
DE   AltName: Full=66 kDa protective minor surface antigen;
DE   Flags: Precursor;
GN   Name=PK66;
OS   Plasmodium knowlesi (strain nuri).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Plasmodium).
OX   NCBI_TaxID=5852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Line W1;
RX   PubMed=2211675; DOI=10.1016/s0021-9258(18)38259-0;
RA   Waters A.P., Thomas A.W., Deans J.A., Mitchell G.H., Hudson D.E.,
RA   Miller L.H., McCutchan T.F., Cohen S.;
RT   "A merozoite receptor protein from Plasmodium knowlesi is highly conserved
RT   and distributed throughout Plasmodium.";
RL   J. Biol. Chem. 265:17974-17979(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NURI;
RX   PubMed=2011149; DOI=10.1016/0166-6851(91)90230-4;
RA   Waters A.P., Thomas A.W., Mitchell G.H., McCutchan T.F.;
RT   "Intra-generic conservation and limited inter-strain variation in a
RT   protective minor surface antigen of Plasmodium knowlesi merozoites.";
RL   Mol. Biochem. Parasitol. 44:141-144(1991).
CC   -!- FUNCTION: Merozoite receptor PK66 is a surface antigen involved in
CC       parasite invasion of erythrocytes.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC       Note=Concentrated at the apical end prior to rupture, following which
CC       it can distribute itself entirely across the surface of the free
CC       merozoite. During invasion PK66 is excluded from the erythrocyte at,
CC       and behind, the invasion interface.
CC   -!- MISCELLANEOUS: PK66 expressed in mature schizonts is rapidly processed
CC       as the schizont ruptures, yielding a 42/44 kDa doublet associated with
CC       the surface merozoite.
CC   -!- SIMILARITY: Belongs to the apicomplexan parasites AMA1 family.
CC       {ECO:0000305}.
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DR   EMBL; M58317; AAA63444.1; -; mRNA.
DR   EMBL; M61097; AAA29728.1; -; Genomic_DNA.
DR   AlphaFoldDB; P21303; -.
DR   SMR; P21303; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.1010.10; -; 1.
DR   InterPro; IPR003298; Apmem_Ag1.
DR   InterPro; IPR024056; Apmem_Ag1_dom_sf.
DR   Pfam; PF02430; AMA-1; 1.
DR   PRINTS; PR01361; MEROZOITESA.
DR   SMART; SM00815; AMA-1; 1.
DR   SUPFAM; SSF82910; SSF82910; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Malaria; Membrane; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..13
FT                   /evidence="ECO:0000255"
FT   CHAIN           14..563
FT                   /note="Merozoite receptor PK66"
FT                   /id="PRO_0000024608"
FT   TOPO_DOM        14..487
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        488..508
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        509..563
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        36
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        107
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        176
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        189
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        238
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        441
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         228
FT                   /note="N -> K (in strain: Line W1)"
SQ   SEQUENCE   563 AA;  64680 MW;  9EF0620264AA5CCF CRC64;
     MNKIYYILFL SAQCLVHMGK CERNQKTTRL TRSANNASLE KGPIIERSIR MSNPWKAFME
     KYDLERAHNS GIRIDLGEDA EVGNSKYRIP AGKCPVFGKG IVIENSNVSF LTPVATGAQR
     LKEGGFAFPN ADDHISPITI ANLKERYKEN ADLMKLNDIA LCKTHAASFV IAEDQNTSYR
     HPAVYDEKNK TCYMLYLSAQ ENMGPRYCSP DSQNKDAMFC FKPDKNENFD NLVYLSKNVS
     NDWENKCPRK NLGNAKFGLW VDGNCEEIPY VNEVEARSLR ECNRIVFEAS ASDQPRQYEE
     ELTDYEKIQE GFRQNNRDMI KSAFLPVGAF NSDNFKSKGR GYNWANFDSV NNKCYIFNTK
     PTCLINDKNF FATTALSHPQ EVDNEFPCSI YKDEIEREIK KQSRNMNLYS VDKERIVLPR
     IFISTDKESI KCPCEPEHIS NSTCNFYVCN CVEKRAEIKE NNEVIIKEEF KEDYENPDGK
     HKKKMLLIII GVTGAVCVVA VASLFYFRKK AQDDKYDKMD QAEAYGKTAN TRKDEMLDPE
     ASFWGEDKRA SHTTPVLMEK PYY