ID   PK81B_PSEFD             Reviewed;         154 AA.
AC   M2ZIX2;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Decarboxylase MYCFIDRAFT_34057 {ECO:0000303|PubMed:30735556};
DE            EC=4.1.1.- {ECO:0000305|PubMed:30735556};
DE   AltName: Full=PKS8-1 gene cluster protein MYCFIDRAFT_34057 {ECO:0000303|PubMed:30735556};
GN   ORFNames=MYCFIDRAFT_34057;
OS   Pseudocercospora fijiensis (strain CIRAD86) (Black leaf streak disease
OS   fungus) (Mycosphaerella fijiensis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora.
OX   NCBI_TaxID=383855;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CIRAD86;
RX   PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA   Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA   Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA   LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA   Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA   Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA   Grigoriev I.V.;
RT   "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT   genomes of eighteen Dothideomycetes fungi.";
RL   PLoS Pathog. 8:E1003037-E1003037(2012).
RN   [2]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=27388157; DOI=10.1371/journal.pone.0158471;
RA   Noar R.D., Daub M.E.;
RT   "Bioinformatics prediction of polyketide synthase gene clusters from
RT   Mycosphaerella fijiensis.";
RL   PLoS ONE 11:e0158471-e0158471(2016).
RN   [3]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=30735556; DOI=10.1371/journal.pone.0212229;
RA   Noar R.D., Thomas E., Daub M.E.;
RT   "A novel polyketide synthase gene cluster in the plant pathogenic fungus
RT   Pseudocercospora fijiensis.";
RL   PLoS ONE 14:e0212229-e0212229(2019).
CC   -!- FUNCTION: Decarboxylase; part of the gene cluster that mediates the
CC       biosynthesis of an emodin derivative that may be involved in black
CC       Sigatoka disease of banana (PubMed:27388157, PubMed:30735556). The
CC       pathway begins with the synthesis of atrochrysone thioester by the
CC       polyketide synthase PKS8-1 (Probable). The atrochrysone carboxyl ACP
CC       thioesterase MYCFIDRAFT_190111 then breaks the thioester bond and
CC       releases the atrochrysone carboxylic acid from PKS8-1 (Probable). The
CC       decarboxylase MYCFIDRAFT_34057 then catalyzes the concerted
CC       decarboxylation-elimination required to convert atochrysone carboxylic
CC       acid into emodin anthrone, which is further oxidized to emodin by the
CC       anthrone oxygenase MYCFIDRAFT_34418 (Probable). The functions of the
CC       other tailoring enzymes as well as the final product of the cluster
CC       have still to be identified (Probable). {ECO:0000269|PubMed:27388157,
CC       ECO:0000269|PubMed:30735556, ECO:0000305|PubMed:30735556}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=atrochrysone carboxylate + H(+) = atrochrysone + CO2;
CC         Xref=Rhea:RHEA:64264, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:149713, ChEBI:CHEBI:150016;
CC         Evidence={ECO:0000305|PubMed:30735556};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64265;
CC         Evidence={ECO:0000305|PubMed:30735556};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:30735556}.
CC   -!- INDUCTION: Expression is positively regulated by the cluster-specific
CC       transcription factor MYCFIDRAFT_198930 and is up-regulated during
CC       banana leaves infection. {ECO:0000269|PubMed:30735556}.
CC   -!- SIMILARITY: Belongs to the tpcK family. {ECO:0000305}.
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DR   EMBL; KB446562; EME79059.1; -; Genomic_DNA.
DR   RefSeq; XP_007929611.1; XM_007931420.1.
DR   AlphaFoldDB; M2ZIX2; -.
DR   EnsemblFungi; EME79059; EME79059; MYCFIDRAFT_34057.
DR   GeneID; 19338972; -.
DR   KEGG; pfj:MYCFIDRAFT_34057; -.
DR   eggNOG; ENOG502RFY1; Eukaryota.
DR   HOGENOM; CLU_115019_0_0_1; -.
DR   OrthoDB; 1621831at2759; -.
DR   Proteomes; UP000016932; Unassembled WGS sequence.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   InterPro; IPR009799; EthD_dom.
DR   Pfam; PF07110; EthD; 1.
DR   SUPFAM; SSF54909; SSF54909; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..154
FT                   /note="Decarboxylase MYCFIDRAFT_34057"
FT                   /id="PRO_0000451119"
SQ   SEQUENCE   154 AA;  17986 MW;  0005BE37F5F418C1 CRC64;
     MPPATDPEIP AVTAPQKNKY LCLTILGYKK EGMTEEAYRN HMLNVSAPMT KDLMVKYGIR
     RWTMIHNQTP TRALMTQLFD SQMVNLAPYD CFSQVVFESI EDYKKIKLDP WYRKYLVGDH
     ENFADTKRSE MTIGWIEEFV RDGQLVEGFE GEYV