PK81C_PSEFD
ID PK81C_PSEFD Reviewed; 173 AA.
AC M3APK4;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=Anthrone oxygenase MYCFIDRAFT_34418 {ECO:0000303|PubMed:30735556};
DE EC=1.10.3.- {ECO:0000305|PubMed:30735556};
DE AltName: Full=PKS8-1 gene cluster protein MYCFIDRAFT_34418 {ECO:0000303|PubMed:30735556};
GN ORFNames=MYCFIDRAFT_34418;
OS Pseudocercospora fijiensis (strain CIRAD86) (Black leaf streak disease
OS fungus) (Mycosphaerella fijiensis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora.
OX NCBI_TaxID=383855;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CIRAD86;
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [2]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=27388157; DOI=10.1371/journal.pone.0158471;
RA Noar R.D., Daub M.E.;
RT "Bioinformatics prediction of polyketide synthase gene clusters from
RT Mycosphaerella fijiensis.";
RL PLoS ONE 11:e0158471-e0158471(2016).
RN [3]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=30735556; DOI=10.1371/journal.pone.0212229;
RA Noar R.D., Thomas E., Daub M.E.;
RT "A novel polyketide synthase gene cluster in the plant pathogenic fungus
RT Pseudocercospora fijiensis.";
RL PLoS ONE 14:e0212229-e0212229(2019).
CC -!- FUNCTION: Anthrone oxygenase; part of the gene cluster that mediates
CC the biosynthesis of an emodin derivative that may be involved in black
CC Sigatoka disease of banana (PubMed:27388157, PubMed:30735556). The
CC pathway begins with the synthesis of atrochrysone thioester by the
CC polyketide synthase PKS8-1 (Probable). The atrochrysone carboxyl ACP
CC thioesterase MYCFIDRAFT_190111 then breaks the thioester bond and
CC releases the atrochrysone carboxylic acid from PKS8-1 (Probable). The
CC decarboxylase MYCFIDRAFT_34057 then catalyzes the concerted
CC decarboxylation-elimination required to convert atochrysone carboxylic
CC acid into emodin anthrone, which is further oxidized to emodin by the
CC anthrone oxygenase MYCFIDRAFT_34418 (Probable). The functions of the
CC other tailoring enzymes as well as the final product of the cluster
CC have still to be identified (Probable). {ECO:0000269|PubMed:27388157,
CC ECO:0000269|PubMed:30735556, ECO:0000305|PubMed:30735556}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=emodin anthrone + O2 = emodin + H(+) + H2O;
CC Xref=Rhea:RHEA:64268, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:77659, ChEBI:CHEBI:150013;
CC Evidence={ECO:0000305|PubMed:30735556};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64269;
CC Evidence={ECO:0000305|PubMed:30735556};
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:30735556}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression is positively regulated by the cluster-specific
CC transcription factor MYCFIDRAFT_198930 and is up-regulated during
CC banana leaves infection. {ECO:0000269|PubMed:30735556}.
CC -!- SIMILARITY: Belongs to the anthrone oxygenase family. {ECO:0000305}.
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DR EMBL; KB446562; EME79058.1; -; Genomic_DNA.
DR RefSeq; XP_007929629.1; XM_007931438.1.
DR AlphaFoldDB; M3APK4; -.
DR EnsemblFungi; EME79058; EME79058; MYCFIDRAFT_34418.
DR GeneID; 19338990; -.
DR KEGG; pfj:MYCFIDRAFT_34418; -.
DR eggNOG; ENOG502SR2H; Eukaryota.
DR HOGENOM; CLU_105974_0_0_1; -.
DR OrthoDB; 1517224at2759; -.
DR Proteomes; UP000016932; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Monooxygenase; Oxidoreductase; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..173
FT /note="Anthrone oxygenase MYCFIDRAFT_34418"
FT /id="PRO_0000451120"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 173 AA; 18978 MW; 75E8A4DB95BDFF3A CRC64;
MKSDTQVRAP TPKVGKQATA VTLGAFLSGA MMSLSAVMIP VVLQTNDQAD QLLKQWALLY
HYGHIIMPSL AILTTSLYAY IAYSKRAVGH QDWSAYAKAG LTTIAIVPFT LTIMEPTNDT
LHELSRSTGN SLDIVQGLIV KWVWMHTVRS VFPMFGSILG FRGVLKECGV YSK
