ID   PK81I_PSEFD             Reviewed;         309 AA.
AC   M2ZIX7;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   07-OCT-2020, sequence version 2.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Short chain dehydrogenase MYCFIDRAFT_6125 {ECO:0000303|PubMed:30735556};
DE            EC=1.1.1.- {ECO:0000305|PubMed:30735556};
DE   AltName: Full=PKS8-1 gene cluster protein MYCFIDRAFT_6125 {ECO:0000303|PubMed:30735556};
GN   ORFNames=MYCFIDRAFT_6125;
OS   Pseudocercospora fijiensis (strain CIRAD86) (Black leaf streak disease
OS   fungus) (Mycosphaerella fijiensis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora.
OX   NCBI_TaxID=383855;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CIRAD86;
RX   PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA   Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA   Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA   LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA   Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA   Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA   Grigoriev I.V.;
RT   "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT   genomes of eighteen Dothideomycetes fungi.";
RL   PLoS Pathog. 8:E1003037-E1003037(2012).
RN   [2]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=27388157; DOI=10.1371/journal.pone.0158471;
RA   Noar R.D., Daub M.E.;
RT   "Bioinformatics prediction of polyketide synthase gene clusters from
RT   Mycosphaerella fijiensis.";
RL   PLoS ONE 11:e0158471-e0158471(2016).
RN   [3]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=30735556; DOI=10.1371/journal.pone.0212229;
RA   Noar R.D., Thomas E., Daub M.E.;
RT   "A novel polyketide synthase gene cluster in the plant pathogenic fungus
RT   Pseudocercospora fijiensis.";
RL   PLoS ONE 14:e0212229-e0212229(2019).
CC   -!- FUNCTION: Short chain dehydrogenase; part of the gene cluster that
CC       mediates the biosynthesis of an emodin derivative that may be involved
CC       in black Sigatoka disease of banana (PubMed:27388157, PubMed:30735556).
CC       The pathway begins with the synthesis of atrochrysone thioester by the
CC       polyketide synthase PKS8-1 (Probable). The atrochrysone carboxyl ACP
CC       thioesterase MYCFIDRAFT_190111 then breaks the thioester bond and
CC       releases the atrochrysone carboxylic acid from PKS8-1 (Probable). The
CC       decarboxylase MYCFIDRAFT_34057 then catalyzes the concerted
CC       decarboxylation-elimination required to convert atochrysone carboxylic
CC       acid into emodin anthrone, which is further oxidized to emodin by the
CC       anthrone oxygenase MYCFIDRAFT_34418 (Probable). The functions of the
CC       other tailoring enzymes as well as the final product of the cluster
CC       have still to be identified (Probable). {ECO:0000269|PubMed:27388157,
CC       ECO:0000269|PubMed:30735556, ECO:0000305|PubMed:30735556}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:30735556}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EME79064.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=EME79064.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; KB446562; EME79064.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_007929584.1; XM_007931393.1.
DR   AlphaFoldDB; M2ZIX7; -.
DR   SMR; M2ZIX7; -.
DR   STRING; 83344.XP_007929584.1; -.
DR   EnsemblFungi; EME79064; EME79064; MYCFIDRAFT_6125.
DR   GeneID; 19340661; -.
DR   KEGG; pfj:MYCFIDRAFT_6125; -.
DR   eggNOG; KOG1205; Eukaryota.
DR   HOGENOM; CLU_010194_2_10_1; -.
DR   OrthoDB; 1194344at2759; -.
DR   Proteomes; UP000016932; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..309
FT                   /note="Short chain dehydrogenase MYCFIDRAFT_6125"
FT                   /id="PRO_0000451126"
FT   ACT_SITE        182
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
SQ   SEQUENCE   309 AA;  32777 MW;  0463A1E7D23E3B52 CRC64;
     MTATYMNSSV PFMYDSYPAV APERFQGQLQ GKTAIVTGGS SGIGRAVCKA FASAGASVAC
     IARREPRLKT LVDEIKAEGG KAIPLAVDIS EKDAAKKIVS QVEAELGPVD ILVNVAGIAR
     LGPLVDEPED LDIWWKVHEV NVRAPALLTR AVLPSMIERK SGAVISVSSV VATWPSPIQT
     AYASSKAAIS KFHESLAAEL EGTGVLSFAL NPGSVESELG APDDAINTAS QHPMLEKMRQ
     MLKSPRKKQT AQLPADVCVA LVCDPRCKVL NGRHVEAAQD LSPVLEEAEK EGGGRIGKDR
     LYLVNIGIL