PKD1_CAEEL
ID PKD1_CAEEL Reviewed; 3284 AA.
AC Q09624; Q09625; Q969D4;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 4.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Location of vulva defective 1;
DE AltName: Full=Polycystic kidney disease 1 protein homolog;
DE AltName: Full=Polycystin-1;
DE Flags: Precursor;
GN Name=lov-1; Synonyms=pkd-1; ORFNames=ZK945.9/ZK945.10;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10517638; DOI=10.1038/43913;
RA Barr M.M., Sternberg P.W.;
RT "A polycystic kidney-disease gene homologue required for male mating
RT behaviour in C. elegans.";
RL Nature 401:386-389(1999).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11553327; DOI=10.1016/s0960-9822(01)00423-7;
RA Barr M.M., DeModena J., Braun D., Nguyen C.Q., Hall D.H., Sternberg P.W.;
RT "The Caenorhabditis elegans autosomal dominant polycystic kidney disease
RT gene homologs lov-1 and pkd-2 act in the same pathway.";
RL Curr. Biol. 11:1341-1346(2001).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12411744; DOI=10.1159/000066650;
RA Kaletta T., Van der Craen M., Van Geel A., Dewulf N., Bogaert T.,
RA Branden M., King K.V., Buechner M., Barstead R., Hyink D., Li H.-P.,
RA Geng L., Burrow C., Wilson P.;
RT "Towards understanding the polycystins.";
RL Nephron Exp. Nephrol. 93:E9-17(2003).
RN [5]
RP FUNCTION.
RX PubMed=15753033; DOI=10.1016/j.cub.2004.12.073;
RA Peden E.M., Barr M.M.;
RT "The KLP-6 kinesin is required for male mating behaviors and polycystin
RT localization in Caenorhabditis elegans.";
RL Curr. Biol. 15:394-404(2005).
RN [6]
RP FUNCTION, INTERACTION WITH ATP-2, AND TISSUE SPECIFICITY.
RX PubMed=15563610; DOI=10.1091/mbc.e04-09-0851;
RA Hu J., Barr M.M.;
RT "ATP-2 interacts with the PLAT domain of LOV-1 and is involved in
RT Caenorhabditis elegans polycystin signaling.";
RL Mol. Biol. Cell 16:458-469(2005).
RN [7]
RP FUNCTION.
RX PubMed=16943275; DOI=10.1242/dev.02555;
RA Bae Y.-K., Qin H., Knobel K.M., Hu J., Rosenbaum J.L., Barr M.M.;
RT "General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia.";
RL Development 133:3859-3870(2006).
RN [8]
RP INTERACTION WITH KIN-10.
RX PubMed=16481400; DOI=10.1091/mbc.e05-10-0935;
RA Hu J., Bae Y.-K., Knobel K.M., Barr M.M.;
RT "Casein kinase II and calcineurin modulate TRPP function and ciliary
RT localization.";
RL Mol. Biol. Cell 17:2200-2211(2006).
RN [9]
RP INTERACTION WITH STAM-1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17581863; DOI=10.1091/mbc.e07-03-0239;
RA Hu J., Wittekind S.G., Barr M.M.;
RT "STAM and Hrs down-regulate ciliary TRP receptors.";
RL Mol. Biol. Cell 18:3277-3289(2007).
CC -!- FUNCTION: Required for two aspects of male mating behavior: response to
CC hermaphrodite contact and vulva location. Acts in the same pathway as
CC pkd-2 and atp-2 in response behavior. May be required for ciliary
CC targeting of pkd-2. {ECO:0000269|PubMed:10517638,
CC ECO:0000269|PubMed:11553327, ECO:0000269|PubMed:12411744,
CC ECO:0000269|PubMed:15563610, ECO:0000269|PubMed:15753033,
CC ECO:0000269|PubMed:16943275}.
CC -!- SUBUNIT: Interacts (via PLAT domain) with atp-2 (via N-terminus) and
CC with kin-10 (via C-terminus). Interacts (via C-terminus) with isoform a
CC of stam-1/pqn-19 (via C-terminus). {ECO:0000269|PubMed:15563610,
CC ECO:0000269|PubMed:16481400, ECO:0000269|PubMed:17581863}.
CC -!- INTERACTION:
CC Q09624; P46561: atp-2; NbExp=4; IntAct=EBI-2529627, EBI-316294;
CC Q09624; P28548: kin-10; NbExp=2; IntAct=EBI-2529627, EBI-317777;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cell projection, cilium
CC {ECO:0000269|PubMed:17581863}. Note=localizes to both the ciliary base
CC and cilium proper.
CC -!- TISSUE SPECIFICITY: Exclusively expressed in a subset of three
CC categories of adult male sensory neurons: ray neurons, hook neurons and
CC head cephalic (CEM) neurons. {ECO:0000269|PubMed:10517638,
CC ECO:0000269|PubMed:11553327, ECO:0000269|PubMed:12411744,
CC ECO:0000269|PubMed:15563610, ECO:0000269|PubMed:17581863}.
CC -!- DEVELOPMENTAL STAGE: First expressed during L4 and peaks in the adult
CC male. {ECO:0000269|PubMed:12411744}.
CC -!- MISCELLANEOUS: Worms lacking lov-1 exhibit defects in two aspects of
CC male mating behavior: response to hermaphrodite contact and vulva
CC location.
CC -!- SIMILARITY: Belongs to the polycystin family. {ECO:0000305}.
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DR EMBL; Z48544; CAB70192.3; -; Genomic_DNA.
DR EMBL; Z48582; CAB70192.3; JOINED; Genomic_DNA.
DR PIR; T28125; T28125.
DR RefSeq; NP_496184.3; NM_063783.3.
DR BioGRID; 39895; 3.
DR ComplexPortal; CPX-3887; TRPP complex.
DR IntAct; Q09624; 3.
DR STRING; 6239.ZK945.9; -.
DR PaxDb; Q09624; -.
DR PRIDE; Q09624; -.
DR EnsemblMetazoa; ZK945.9.1; ZK945.9.1; WBGene00003058.
DR GeneID; 174576; -.
DR KEGG; cel:CELE_ZK945.9; -.
DR CTD; 174576; -.
DR WormBase; ZK945.9; CE45696; WBGene00003058; lov-1.
DR eggNOG; KOG3599; Eukaryota.
DR GeneTree; ENSGT00940000164047; -.
DR HOGENOM; CLU_000354_0_0_1; -.
DR InParanoid; Q09624; -.
DR OMA; WDWARES; -.
DR OrthoDB; 1276906at2759; -.
DR PRO; PR:Q09624; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003058; Expressed in material anatomical entity and 2 other tissues.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; IDA:WormBase.
DR GO; GO:0097730; C:non-motile cilium; IDA:WormBase.
DR GO; GO:0071683; C:sensory dendrite; IC:ComplexPortal.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0019904; F:protein domain specific binding; IPI:WormBase.
DR GO; GO:0050982; P:detection of mechanical stimulus; IBA:GO_Central.
DR GO; GO:0060179; P:male mating behavior; IMP:UniProtKB.
DR GO; GO:0007617; P:mating behavior; IMP:WormBase.
DR GO; GO:1902435; P:regulation of male mating behavior; IC:ComplexPortal.
DR GO; GO:0034606; P:response to hermaphrodite contact; IMP:UniProtKB.
DR GO; GO:0034608; P:vulval location; IMP:UniProtKB.
DR CDD; cd01752; PLAT_polycystin; 1.
DR InterPro; IPR000203; GPS.
DR InterPro; IPR013122; PKD1_2_channel.
DR InterPro; IPR001024; PLAT/LH2_dom.
DR InterPro; IPR036392; PLAT/LH2_dom_sf.
DR InterPro; IPR042060; PLAT_polycystin1.
DR Pfam; PF08016; PKD_channel; 1.
DR Pfam; PF01477; PLAT; 1.
DR SMART; SM00303; GPS; 1.
DR SMART; SM00308; LH2; 1.
DR SUPFAM; SSF49723; SSF49723; 1.
DR PROSITE; PS50095; PLAT; 1.
PE 1: Evidence at protein level;
KW Behavior; Cell projection; Cilium; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..3284
FT /note="Location of vulva defective 1"
FT /id="PRO_0000065555"
FT TRANSMEM 2245..2265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2453..2473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2496..2516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2557..2577
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2592..2612
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2672..2692
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2945..2965
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 2994..3014
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 3043..3063
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 3089..3109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 3144..3164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 2177..2226
FT /note="GPS"
FT DOMAIN 2288..2411
FT /note="PLAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00152"
FT REGION 226..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 350..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 623..702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 827..926
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1043..1216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3284 AA; 355492 MW; 1E2E5320857CED2F CRC64;
MKKSNFFVLL LLAISAIQID GLHYQLLDGI ATFRLDNDDT TIGGVPRNSQ GVVKIKLSCG
LNRLSVENKV TEVSSLELIH NCIQTETRLV GLFLNSTWIT LNEVNDDDEI SIAVEAKYEV
CYDDGIDRCD GSLWWLQVGG NEMALLGYRE KCESGEINEE YARRMCKRPY RSEKSTAISD
SQGVYYDGQV LKGVRAKQFS MRTSGSPTLR RMKRDAGDNT CDYTIESTST STTTPTTTTV
TSTVTSTTTV PTSTSTVTTA MSTSTSTPST STTIESTSTT FTSTASTSTS STSTTQQSSS
TITSSPSSTT LSTSIPTTTT PEITSTLSSL PDNAICSYLD ETTTSTTFTT TMLTSTTTEE
PSTSTTTTEV TSTSSTVTTT EPTTTLTTST ASTSTTEPST STVTTSPSTS PVTSTVTSSS
SSSTTVTTPT STESTSTSPS STVTTSTTAP STSTTGPSSS SSTPSSTASS SVSSTASSTQ
SSTSTQQSST TTKSETTTSS DGTNPDFYFV EKATTTFYDS TSVNLTLNSG LGIIGYQTSI
ECTSPTSSNY VSTTKDGACF TKSVSMPRLG GTYPASTFVG PGNYTFRATM TTDDKKVYYT
YANVYIQEYS STTIESESST SAVASSTSST PSTPSSTLST STVTEPSSTR SSDSTTTSAG
STTTLQESTT TSEESTTDSS TTTISDTSTT SSSPSSTTAD STSTLSVDQF DFILDSGLSW
NETRHNEDSI NIVPLPTNAI TPTERSQTFE CRNVSTEPFL IIKESTCLNY SNTVLNATYS
SNIPIQPIET FLVGIGTYEF RINMTDLTTM QVVSHIFTLN VVADSTSTSE VTSTTSTGSS
SESSAISTTS GIESTSTLEA STTDASQDSS TSTSDSGTTS DSTTIDSSNS TPSTSDSSGL
SQTPSDSSSA SDSMRTTTVD PDASTETPYD FVLENLTWNE TVYYSENPFY ITPIPNKEPG
ALTTAMTCQC RNDSSQPFVL LKESNCLTEF GKNGAYSASV SFNPMTSFVP ATGTYEFLIN
VTNRASGESA SHIFTMNVVL PTTTTETPPT TVSSSDDAGG KTGGTGATGG TGGTGSGGSA
TTLSTGDAVR STTSGSGSGQ SSTGSGAGGS GTTASGSGSG GSSGTGSDGV NSGKTTALNG
DGTGSGTATT PGSHLGDGGS TSGSGSDSNG SSGVSTKSSS GSDTSGSSDS SGANGAFSAT
AQPSTRTTKT RSSLATVSPI SAAEQAIIDA QKADVMNQLA GIMDGSASNN SLNTSSSLLN
QISSLPAADL VEVAQSLLSN TLKIPGVGNM SSVDVLKTLQ DNIATTNSEL ADEMAKVITK
LANVNMTSAQ SLNSVLSSLD LALKGSTVYT LGVSSTKSKD GTYAVIFGYV IASGYTLVSP
RCTLSIYGST IYLTGDTRAS YKQLDGDTVT ADTMLAAAIG IQGMFATNGR TVQVEQDKID
DKRSLVSGNI MATMSGVGDV QSGEYSYNDM YVTAWNVTYD NSTVGSTSQK NTSFSFNIPV
SEVQYILLIE SGTMIKLHST QNIVSRGLVV TASYGGVTYT ITCTNGTGKF VEVDTDNAIF
SYNADSFTVV ASDGSSASTV KKLIQMPIVI ENVNLALFNQ TTSPLVFSNA GSYSMRMVLS
PQDIGIPAVS ALSQTVSIST LSPTASYTKD DLQSLIKEQT LVTVSGTLFF SKASSIDVSG
YSFFVDSTAL YLSNSVTTLV ISSPTYNIVS LALGGYGIQI TAGTYTSGSQ THTVTLMEFS
DTQKMRIDGG LIIRNGTNGY VIQNGQVSTE GDVSGTKIDI VPQSLMNQES QQQIETILSN
TQDFLTNNGM TMTDAEINDT SNSLLSIAGS LTSALKIALD NPLSSDLAAN LKYATDNYDS
LYNVLPSDPD NIVYVEEMTS EEWAAYVTKM FQKNIAKNLA NQLASTLDTL ENTLAARAIA
TGNLPYDYSN SVDGTGMVIV IDDASNIVGK TQNCEEWAFK LPSPASTLNT AEITDKTLIQ
VGLVCYATNP RTYVDNFDML ITSGALEAHI KDENQIIIPI TGTTAPIYVN GRGSEDDAVL
TLMQQGDFAS YQILDLHAFR TTNWNNSLQV EIIASQDYEI PNNDDTYMFS SFQSLPGPLE
SNHEWIFDLN TLNKTSNYFV TAGNLINNTG LFFIGIGKRN SSTNTGNSSD IVNYGQYDSM
QWSFARSVPM DYQVAAVSKG CYFYQKTSDV FNSEGMYPSD GQGMQFVNCS TDHLTMFSVG
AFNPTIDADF SYNYNVNEIE KNVKVMIAAV FMLVVYGCLT INAIICQRKD ASRGRLRFLK
DNEPHDGYMY VIAVETGYRM FATTDSTICF NLSGNEGDQI FRSFRSEEDG NWEFPFSWGT
TDRFVMTTAF PLGELEYMRL WLDDAGLDHR ESWYCNRIIV KDLQTQDIYY FPFNNWLGTK
NGDGETERLA RVEYKRRFLD ESMSMHMLAQ TISWFAMFTG GGNRLRDRVS RQDYSVSIIF
SLVVVSMISI TILKSDNSII SDSKSVSEFT FTIKDIAFGV GFGVLITFLN SLHILLCTKC
RSHSEHYYYK KRKREDPEFK DNSGSWPMFM AGMARTIIVF PVLMGLIYIS GAGMSLMDDL
ANSFYIRFLI SLILWAVVFE PIKGLIWAFL ILKTRKSHKI INKLEEALLR AKPAETFLRN
PYGKIEKGLG TEIADVTKLR DTENRKMRDE QLFITIRDML CFFASLYIMV MLTYYCKDRH
GYWYQLEMST ILNINQKNYG DNTFMSIQHA DDFWDWARES LATALLASWY DGNPAYGMRA
YMNDKVSRSM GIGTIRQVRT KKSAECTMFK QFQGYINDCG EELTSKNEEK TLYMQAGWTE
LESENGTDAS DEYTYKTSEE LSTETVSGLL YSYSGGGYTI SMSGTQAEII TLFNKLDSER
WIDDHTRAVI IEFSAYNAQI NYFSVVQLLV EIPKSGIYLP NSWVESVRLI KSEGSDGTVV
KYYEMLYIFF SVLIFVKEIV FYLYGRYKVI TTMKPTRNPF KIVYQLALGN FSPWNFMDLI
VGALAVASVL AYTIRQRTTN RAMEDFNANN GNSYINLTEQ RNWEIVFSYC LAGAVFFTSC
KMIRILRFNR RIGVLAATLD NALGAIVSFG IAFLFFSMTF NSVLYAVLGN KMGGYRSLMA
TFQTALAGML GKLDVTSIQP ISQFAFVVIM LYMIAGSKLV LQLYVTIIMF EFEEIRNDSE
KQTNDYEIID HIKYKTKRRL GLLEPKDFAP VSIADTQKDF RLFHSAVAKV NLLHHRATRM
LQTQGQYQNQ TVINYTLSYD PVSAIHETGP KRFQKWRLND VEKD
