ID   PKG16_BPPZA             Reviewed;         332 AA.
AC   P07541;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=DNA packaging protein {ECO:0000250|UniProtKB:P11014};
DE   AltName: Full=ATPase gp16 {ECO:0000250|UniProtKB:P11014};
DE            EC=3.6.4.- {ECO:0000250|UniProtKB:P11014};
DE   AltName: Full=Gene product 16;
DE            Short=gp16;
DE   AltName: Full=Protein p16;
GN   Name=16;
OS   Bacillus phage PZA (Bacteriophage PZA).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Salasmaviridae; Picovirinae; Salasvirus; Bacillus virus PZA.
OX   NCBI_TaxID=10757;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3095188; DOI=10.1016/0378-1119(86)90048-x;
RA   Paces V., Vlcek C., Urbanek P.;
RT   "Nucleotide sequence of the late region of Bacillus subtilis phage PZA, a
RT   close relative of phi 29.";
RL   Gene 44:107-114(1986).
CC   -!- FUNCTION: ATPase required for the genome encapsidation reaction. Part
CC       of the active packaging motor via the binding to the packaging RNA
CC       (pRNA), itself fixed to the head-tail connector at the unique portal
CC       vertex of the prohead. Binds and supercoils the DNA-gp3 to produce an
CC       initiation complex for DNA packaging. Provides the energy to actively
CC       pump the viral DNA into the prohead. Approximately one molecule of ATP
CC       is used in the packaging of 2 bp of viral DNA. After packaging, the
CC       ATPase and the pRNA are released from the prohead.
CC       {ECO:0000250|UniProtKB:P11014}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
CC         Evidence={ECO:0000250|UniProtKB:P11014};
CC   -!- SUBUNIT: Homohexamer. Interacts with the packaging RNA. Part of a DNA-
CC       gp3-gp16 complex. {ECO:0000250|UniProtKB:P11014}.
CC   -!- SIMILARITY: Belongs to the phi29likevirus gp16 family. {ECO:0000305}.
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DR   EMBL; M11813; AAA88493.1; -; Genomic_DNA.
DR   PIR; B26215; WMBP16.
DR   SMR; P07541; -.
DR   Proteomes; UP000000855; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR008784; Podovirus_Gp16.
DR   Pfam; PF05894; Podovirus_Gp16; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Hydrolase; Late protein; Nucleotide-binding;
KW   Viral genome packaging; Viral release from host cell.
FT   CHAIN           1..332
FT                   /note="DNA packaging protein"
FT                   /id="PRO_0000106602"
FT   BINDING         24..31
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   332 AA;  38959 MW;  3B8099FD0AE994FA CRC64;
     MDKSLFYNPQ KMLSYDRILN FVIGARGIGK SYAMKVYPIN RFIKYGEQFI YVRRYKPELA
     KVSNYFNDVA QEFPDHELVV KGRRFYIDGK LAGWAIPLSV WQSEKSNAYP NVSTIVFDEF
     IREKDNSNYI PNEVSALLNL MDTVFRNRER VRCICLSNAV SVVNPYFLFF NLVPDVNKRF
     NVYDDALIEI PDSLDFSSER RKTRFGRLID GTEYGEMSLD NQFIGDSHVF IEKRSKDSKF
     VFSIVYNGFT LGVWVDVNQG LMYVDTAHDP STKNVYTLTT DDLNENMMLI TNYKNNYHLR
     KLASAFMNGY LRFDNQVIRN IAYELFRKMR IQ