PKG1_ADE40
ID PKG1_ADE40 Reviewed; 446 AA.
AC P48752;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Packaging protein 1 {ECO:0000255|HAMAP-Rule:MF_04057};
DE AltName: Full=Packaging protein IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
GN Name=IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
OS Human adenovirus F serotype 40 (HAdV-40) (Human adenovirus 40).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus; Human mastadenovirus F.
OX NCBI_TaxID=28284;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Dugan;
RX PubMed=8263936; DOI=10.1006/jmbi.1993.1687;
RA Davison A.J., Telford E.A., Watson M.S., McBride K., Mautner V.;
RT "The DNA sequence of adenovirus type 40.";
RL J. Mol. Biol. 234:1308-1316(1993).
CC -!- FUNCTION: Component of the packaging machinery which encapsidates the
CC viral DNA into preformed capsids and transcriptional activator of the
CC viral major late promoter (MLP). Binds, along with packaging proteins 2
CC and 3, to the specific packaging sequence on the left end of viral
CC genomic DNA and displays ATPase activity thereby providing the power
CC stroke of the packaging machinery. The activity of packaging protein
CC IVa2 is stimulated by protein 33K which acts as a terminase. May be the
CC protein that pumps DNA into the capsid powered by ATP hydrolysis.
CC Specifically binds to the 5'-CG-3' nucleotides of the repeats making up
CC the packaging sequence. Component of the DEF-A and DEF-B transcription
CC factors that bind downstream elements of the major late promoter (MLP),
CC and stimulate transcription from the MLP after initiation of viral DNA
CC replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-
CC B is a homodimer of packaging protein 1. {ECO:0000255|HAMAP-
CC Rule:MF_04057}.
CC -!- SUBUNIT: Homodimer. Part of a genome packaging complex composed of
CC packaging proteins 1, 2 and 3; this complex specifically binds to the
CC packaging sequence on the left end of viral genomic DNA and performs
CC packaging of the viral genome. Interacts with protein 33K.
CC {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC nucleus, host nucleoplasm {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC nucleus, host nucleolus {ECO:0000255|HAMAP-Rule:MF_04057}. Note=Located
CC at a unique vertex of the capsid. Present in about 6-8 copies per
CC virion. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- INDUCTION: Expressed in the intermediate phase of the viral replicative
CC cycle. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- SIMILARITY: Belongs to the adenoviridae packaging protein 1 family.
CC {ECO:0000255|HAMAP-Rule:MF_04057}.
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DR EMBL; L19443; AAC13952.1; -; Genomic_DNA.
DR RefSeq; NP_040852.1; NC_001454.1.
DR PRIDE; P48752; -.
DR GeneID; 2715937; -.
DR KEGG; vg:2715937; -.
DR Proteomes; UP000151954; Genome.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0044095; C:host cell nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0039708; P:nuclear capsid assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0098035; P:viral DNA genome packaging via site-specific sequence recognition; IEA:UniProtKB-UniRule.
DR GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04057; ADV_PKG1; 1.
DR InterPro; IPR003389; Adeno_IVa2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02456; Adeno_IVa2; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; DNA-binding; Host nucleus; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Viral genome packaging;
KW Viral release from host cell; Virion.
FT CHAIN 1..446
FT /note="Packaging protein 1"
FT /id="PRO_0000221887"
FT REGION 1..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 439..446
FT /note="DNA-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 170..177
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
SQ SEQUENCE 446 AA; 51122 MW; C0969E192524A36E CRC64;
METRGRRRAF SHQQDEPEEN PGKRPTRSAP LYRHRNQPNA NPATLERHYP CSTGRPPTGT
VQPKPSQPPQ PRSLLDRDAI DHITELWDRL YLLRQSLEKM TMADGLKPLK HFRSLEELLS
LGGERLLQDL VKENQHVRSM MNEVTPLLRE DGSCISLNYQ LQPVIGVIYG PTGCGKSQLL
RNLLSTQLIN PPPETVFFIA PQVDMIPPSE IKAWEMQICE GNYAPGPEGT IIPQSGTLLP
RFIKMAYDEL TLEQNYDVSH PDNIFAKAAS QGPIAIIMDE CMENLGGHKG VSKFFHAFPS
KLHDKFPKCT GYTVLVVLHN MNPRRDLGGN IANLKIQAKM HLISPRMHPS QLNRFVNTFT
KGLPLAISLL LKDIFQFHAQ KPCYDWIIYN TTPEHDALQW SYLHPRDGLM PMYLNIQAHL
YRVLENIHKV LNDRDRWSRA YRKRNK
