ID   PKG1_ADECR              Reviewed;         502 AA.
AC   Q96680;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Packaging protein 1 {ECO:0000255|HAMAP-Rule:MF_04057};
DE   AltName: Full=Packaging protein IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
GN   Name=IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
OS   Canine adenovirus serotype 1 (strain RI261) (CAdV-1) (Canine adenovirus 1
OS   (strain RI261)).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus; Canine mastadenovirus A.
OX   NCBI_TaxID=69151;
OH   NCBI_TaxID=9615; Canis lupus familiaris (Dog) (Canis familiaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9129661; DOI=10.1099/0022-1317-78-4-873;
RA   Morrison M.D., Onions D.E., Nicolson L.;
RT   "Complete DNA sequence of canine adenovirus type 1.";
RL   J. Gen. Virol. 78:873-878(1997).
CC   -!- FUNCTION: Component of the packaging machinery which encapsidates the
CC       viral DNA into preformed capsids and transcriptional activator of the
CC       viral major late promoter (MLP). Binds, along with packaging proteins 2
CC       and 3, to the specific packaging sequence on the left end of viral
CC       genomic DNA and displays ATPase activity thereby providing the power
CC       stroke of the packaging machinery. The activity of packaging protein
CC       IVa2 is stimulated by protein 33K which acts as a terminase. May be the
CC       protein that pumps DNA into the capsid powered by ATP hydrolysis.
CC       Specifically binds to the 5'-CG-3' nucleotides of the repeats making up
CC       the packaging sequence. Component of the DEF-A and DEF-B transcription
CC       factors that bind downstream elements of the major late promoter (MLP),
CC       and stimulate transcription from the MLP after initiation of viral DNA
CC       replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-
CC       B is a homodimer of packaging protein 1. {ECO:0000255|HAMAP-
CC       Rule:MF_04057}.
CC   -!- SUBUNIT: Homodimer. Part of a genome packaging complex composed of
CC       packaging proteins 1, 2 and 3; this complex specifically binds to the
CC       packaging sequence on the left end of viral genomic DNA and performs
CC       packaging of the viral genome. Interacts with protein 33K.
CC       {ECO:0000255|HAMAP-Rule:MF_04057}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC       nucleus, host nucleoplasm {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC       nucleus, host nucleolus {ECO:0000255|HAMAP-Rule:MF_04057}. Note=Located
CC       at a unique vertex of the capsid. Present in about 6-8 copies per
CC       virion. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC   -!- INDUCTION: Expressed in the intermediate phase of the viral replicative
CC       cycle. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC   -!- SIMILARITY: Belongs to the adenoviridae packaging protein 1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04057}.
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DR   EMBL; Y07760; CAA69056.1; -; Genomic_DNA.
DR   RefSeq; NP_044188.1; NC_001734.1.
DR   GeneID; 1488950; -.
DR   KEGG; vg:1488950; -.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044095; C:host cell nucleoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0039708; P:nuclear capsid assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0098035; P:viral DNA genome packaging via site-specific sequence recognition; IEA:UniProtKB-UniRule.
DR   GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR   GO; GO:0019083; P:viral transcription; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04057; ADV_PKG1; 1.
DR   InterPro; IPR003389; Adeno_IVa2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF02456; Adeno_IVa2; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; DNA-binding; Host nucleus; Nucleotide-binding;
KW   Phosphoprotein; Transcription; Transcription regulation;
KW   Viral genome packaging; Viral release from host cell; Virion.
FT   CHAIN           1..502
FT                   /note="Packaging protein 1"
FT                   /id="PRO_0000221889"
FT   REGION          99..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          495..502
FT                   /note="DNA-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
FT   COMPBIAS        106..122
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         226..233
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
SQ   SEQUENCE   502 AA;  56819 MW;  46F73C5789A00AEA CRC64;
     MWGGCIQFRI SVPGAKIICF KGHHMSRMSI RGQGQVTGKR SCHLHPLLRC AKGLLLCRPA
     AGLRHLQNQQ NEPSQDPHQC ADSCPALHSD RNHLNKEAEA MEGQNPTCSR HESAYPIQSQ
     VSKSKKQRNY VDGAAVDDLK NLWDRLQTLQ QSLTEMPYAE GLKPLKNFTS FEELLSMGGD
     SLLNDLLDIQ DSITQCCIRA SKYLNKEGNC TSLNYYKQPF IAAVYGPTGC GKSQLLRNLM
     SAQLIVPTPE TVFFITPQVD MIPPQEIAAW ETQICEGNFL AGPENTVVPQ SGSIMPRFIQ
     MSYAQLTKDE NYDVTSPNNI FANAASKGPI AIVMDECMED LGGNRGIAKF FHAFPSKLLD
     RFPKCTGYSV IVVLHNMNPR RDQGGNIANL KIQAKVHMIS PKVHPSQLNR FINIYTKGQP
     TAISLLLKDI FNYHRLNTNF DWIVYNTEPI DNCMHWMYLS PQEGLIPMYL NIQGKLYQAL
     ERIHRTLTDR QRWTRYYHSK KK