PKG1_ADES1
ID PKG1_ADES1 Reviewed; 443 AA.
AC A9CB86;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 03-AUG-2022, entry version 45.
DE RecName: Full=Packaging protein 1 {ECO:0000255|HAMAP-Rule:MF_04057};
DE AltName: Full=Packaging protein IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
GN Name=IVa2 {ECO:0000255|HAMAP-Rule:MF_04057};
OS Snake adenovirus serotype 1 (SnAdV-1).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Atadenovirus.
OX NCBI_TaxID=189830;
OH NCBI_TaxID=94885; Pantherophis guttatus (Corn snake) (Elaphe guttata).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12237421; DOI=10.1099/0022-1317-83-10-2403;
RA Farkas S.L., Benko M., Elo P.T., Ursu K., Dan A., Ahne W., Harrach B.;
RT "Genetic analysis of an adenovirus isolated from corn snake (Elaphe
RT guttata) implies common origin with the members of the proposed new genus
RT Atadenovirus.";
RL J. Gen. Virol. 83:2403-2410(2002).
CC -!- FUNCTION: Component of the packaging machinery which encapsidates the
CC viral DNA into preformed capsids and transcriptional activator of the
CC viral major late promoter (MLP). Binds, along with packaging proteins 2
CC and 3, to the specific packaging sequence on the left end of viral
CC genomic DNA and displays ATPase activity thereby providing the power
CC stroke of the packaging machinery. The activity of packaging protein
CC IVa2 is stimulated by protein 33K which acts as a terminase. May be the
CC protein that pumps DNA into the capsid powered by ATP hydrolysis.
CC Specifically binds to the 5'-CG-3' nucleotides of the repeats making up
CC the packaging sequence. Component of the DEF-A and DEF-B transcription
CC factors that bind downstream elements of the major late promoter (MLP),
CC and stimulate transcription from the MLP after initiation of viral DNA
CC replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-
CC B is a homodimer of packaging protein 1. {ECO:0000255|HAMAP-
CC Rule:MF_04057}.
CC -!- SUBUNIT: Homodimer. Part of a genome packaging complex composed of
CC packaging proteins 1, 2 and 3; this complex specifically binds to the
CC packaging sequence on the left end of viral genomic DNA and performs
CC packaging of the viral genome. Interacts with protein 33K.
CC {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC nucleus, host nucleoplasm {ECO:0000255|HAMAP-Rule:MF_04057}. Host
CC nucleus, host nucleolus {ECO:0000255|HAMAP-Rule:MF_04057}. Note=Located
CC at a unique vertex of the capsid. Present in about 6-8 copies per
CC virion. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- INDUCTION: Expressed in the intermediate phase of the viral replicative
CC cycle. {ECO:0000255|HAMAP-Rule:MF_04057}.
CC -!- SIMILARITY: Belongs to the adenoviridae packaging protein 1 family.
CC {ECO:0000255|HAMAP-Rule:MF_04057}.
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DR EMBL; DQ106414; ABA47236.1; -; Genomic_DNA.
DR RefSeq; YP_001552246.1; NC_009989.1.
DR GeneID; 10973888; -.
DR KEGG; vg:10973888; -.
DR Proteomes; UP000136605; Genome.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0044095; C:host cell nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0039708; P:nuclear capsid assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0098035; P:viral DNA genome packaging via site-specific sequence recognition; IEA:UniProtKB-UniRule.
DR GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04057; ADV_PKG1; 1.
DR InterPro; IPR003389; Adeno_IVa2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02456; Adeno_IVa2; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; DNA-binding; Host nucleus; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Viral genome packaging;
KW Viral release from host cell; Virion.
FT CHAIN 1..443
FT /note="Packaging protein 1"
FT /id="PRO_0000425925"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 433..443
FT /note="DNA-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
FT COMPBIAS 12..58
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 169..176
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04057"
SQ SEQUENCE 443 AA; 49956 MW; 82335652C8810845 CRC64;
MSGAADGTVP VGEDTHQEDS GERECEQRPV HSGREATGES DPALERPDHG ERHGPEPRFP
SFSVRPEAAQ PPPSRTIARE SFLGRFITDC LKWKNDVVKI DPNICKDPFP SDDEIFGNIG
SHSTLLRELH ECAYRYQKAV NASHPLLQRD GSLKTLNYGV QPFIVTVYGP TGSGKSQFLR
NVISSKMIDP PPETVFFVTP EKGTVTNEEK LSWEAQCAEG VYNSKCVPIT KTFQPAFVNL
SFSEAVDEEN LSIDSPNNVF VQAAKKGPIC IIIDECMNQL GACRSISSFF HALPSKIFGR
FPACTGYTVL VVLHNMNPRS DRGNIKDLKI QSKCHIISPQ LESQQVSRFI KNFSFGFPTP
LVSVIKDIVD HAKMHSKFSW LVYCSVPVRE SFRWSYYSPE DQLTPLYVDL QAVMYEACHN
IRRVFCKRLY SRVSYANKRK WYD
