ID   PKG20_BPPRD             Reviewed;          42 AA.
AC   P27387; Q3T4N3;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Packaging protein P20;
GN   Name=XX; Synonyms=J;
OS   Enterobacteria phage PRD1 (Bacteriophage PRD1).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Kalamavirales; Tectiviridae; Alphatectivirus.
OX   NCBI_TaxID=10658;
OH   NCBI_TaxID=471; Acinetobacter calcoaceticus.
OH   NCBI_TaxID=562; Escherichia coli.
OH   NCBI_TaxID=584; Proteus mirabilis.
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
OH   NCBI_TaxID=294; Pseudomonas fluorescens.
OH   NCBI_TaxID=303; Pseudomonas putida (Arthrobacter siderocapsulatus).
OH   NCBI_TaxID=90371; Salmonella typhimurium.
OH   NCBI_TaxID=666; Vibrio cholerae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1853567; DOI=10.1016/0042-6822(91)90995-n;
RA   Bamford J.K.H., Haenninen A.-L., Pakula T.M., Ojala P.M., Kalkkinen N.,
RA   Frilander M., Bamford D.H.;
RT   "Genome organization of membrane-containing bacteriophage PRD1.";
RL   Virology 183:658-676(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15946683; DOI=10.1016/j.jmb.2005.04.059;
RA   Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L.,
RA   Bamford D.H., Bamford J.K.H.;
RT   "A snapshot of viral evolution from genome analysis of the tectiviridae
RT   family.";
RL   J. Mol. Biol. 350:427-440(2005).
RN   [3]
RP   SUBCELLULAR LOCATION, AND IDENTIFICATION IN THE PORTAL COMPLEX.
RX   PubMed=12743288; DOI=10.1128/jvi.77.11.6314-6321.2003;
RA   Stromsten N.J., Bamford D.H., Bamford J.K.H.;
RT   "The unique vertex of bacterial virus PRD1 is connected to the viral
RT   internal membrane.";
RL   J. Virol. 77:6314-6321(2003).
RN   [4]
RP   FUNCTION, SUBUNIT, AND IDENTIFICATION IN THE PORTAL COMPLEX.
RX   PubMed=25514469; DOI=10.1371/journal.pbio.1002024;
RA   Hong C., Oksanen H.M., Liu X., Jakana J., Bamford D.H., Chiu W.;
RT   "A structural model of the genome packaging process in a membrane-
RT   containing double stranded DNA virus.";
RL   PLoS Biol. 12:E1002024-E1002024(2014).
CC   -!- FUNCTION: Together with P22, forms the internal part of the portal
CC       complex embeded in the virion internal membrane and which plays
CC       critical roles in genome packaging and genome ejection. Both proteins
CC       multimerize as a single ring-shaped heterdodecamer arranged around a
CC       central channel and interact with the P6/P9 external part of the
CC       portal. {ECO:0000269|PubMed:25514469}.
CC   -!- SUBUNIT: Heterodimer of P20 and P22; further multimerizes as hexamers
CC       of heterodimers. Part of the dodecameric portal complex that is
CC       composed of the packaging efficiency factor P6, the DNA packaging
CC       ATPase P9, and the internal heterododecamer P20/P22 which spans the
CC       virion inner membrane. {ECO:0000269|PubMed:12743288,
CC       ECO:0000269|PubMed:25514469}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass membrane protein.
CC       Note=Part of the capsid inner membrane. Binds the portal complex at the
CC       capsid vertex. {ECO:0000269|PubMed:12743288}.
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DR   EMBL; AY848689; AAX45914.1; -; Genomic_DNA.
DR   PIR; B36776; WMBPPJ.
DR   RefSeq; NP_040691.1; NC_001421.2.
DR   SMR; P27387; -.
DR   Proteomes; UP000002143; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039641; C:viral inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Capsid inner membrane protein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Viral genome packaging; Viral release from host cell;
KW   Virion.
FT   CHAIN           1..42
FT                   /note="Packaging protein P20"
FT                   /id="PRO_0000165365"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   42 AA;  4671 MW;  E1BB890A4897A3EE CRC64;
     MVNWELLKNP INWLIVILML TIAGMAATLV CNHFGKNAVT SE