PKG2_ADE02
ID PKG2_ADE02 Reviewed; 195 AA.
AC P0DJX1;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2013, sequence version 1.
DT 02-JUN-2021, entry version 29.
DE RecName: Full=Packaging protein 2;
DE AltName: Full=Packaging protein 22K;
DE Short=L4-22K;
GN ORFNames=L4;
OS Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus.
OX NCBI_TaxID=10515;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=478297; DOI=10.1016/0378-1119(79)90081-7;
RA Galibert F., Herisse J., Courtois G.;
RT "Nucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome.";
RL Gene 6:1-22(1979).
RN [2]
RP FUNCTION.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=16809303; DOI=10.1128/jvi.00123-06;
RA Ostapchuk P., Anderson M.E., Chandrasekhar S., Hearing P.;
RT "The L4 22-kilodalton protein plays a role in packaging of the adenovirus
RT genome.";
RL J. Virol. 80:6973-6981(2006).
RN [3]
RP INTERACTION WITH PACKAGING PROTEIN 1.
RX PubMed=17804492; DOI=10.1128/jvi.01470-07;
RA Ewing S.G., Byrd S.A., Christensen J.B., Tyler R.E., Imperiale M.J.;
RT "Ternary complex formation on the adenovirus packaging sequence by the IVa2
RT and L4 22-kilodalton proteins.";
RL J. Virol. 81:12450-12457(2007).
RN [4]
RP REVIEW, AND ALTERNATIVE SPLICING.
RX PubMed=18508565; DOI=10.2741/3059;
RA Akusjarvi G.;
RT "Temporal regulation of adenovirus major late alternative RNA splicing.";
RL Front. Biosci. 13:5006-5015(2008).
RN [5]
RP FUNCTION.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=19176628; DOI=10.1128/jvi.02455-08;
RA Morris S.J., Leppard K.N.;
RT "Adenovirus serotype 5 L4-22K and L4-33K proteins have distinct functions
RT in regulating late gene expression.";
RL J. Virol. 83:3049-3058(2009).
RN [6]
RP FUNCTION.
RX PubMed=20621673; DOI=10.1016/j.virusres.2010.05.013;
RA Backstrom E., Kaufmann K.B., Lan X., Akusjarvi G.;
RT "Adenovirus L4-22K stimulates major late transcription by a mechanism
RT requiring the intragenic late-specific transcription factor-binding site.";
RL Virus Res. 151:220-228(2010).
RN [7]
RP SUBUNIT.
RX PubMed=20925313; DOI=10.1021/bi101144q;
RA Yang T.-C., Maluf N.K.;
RT "Self-association of the adenoviral L4-22K protein.";
RL Biochemistry 49:9830-9838(2010).
RN [8]
RP FUNCTION, AND IDENTIFICATION IN THE PACKAGING COMPLEX.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=22811519; DOI=10.1128/jvi.01463-12;
RA Wu K., Orozco D., Hearing P.;
RT "The adenovirus L4-22K Protein is multifunctional and is an integral
RT component of crucial aspects of infection.";
RL J. Virol. 86:10474-10483(2012).
RN [9]
RP SUBCELLULAR LOCATION.
RC STRAIN=Human adenovirus C serotype 5;
RX PubMed=22944109; DOI=10.1016/j.virol.2012.08.021;
RA Ostberg S., Tormanen Persson H., Akusjarvi G.;
RT "Serine 192 in the tiny RS repeat of the adenoviral L4-33K splicing
RT enhancer protein is essential for function and reorganization of the
RT protein to the periphery of viral replication centers.";
RL Virology 433:273-281(2012).
CC -!- FUNCTION: Component of the packaging machinery which encapsidates the
CC viral DNA into preformed capsids and transcriptional activator of the
CC viral major late promoter (MLP). Binds, along with packaging proteins 1
CC and 3, to the specific packaging sequence on the left end of viral
CC genomic DNA and plays an active role in packaging of the viral genome
CC into preformed capsids. Specifically binds to the 5'-TTTG-3'
CC nucleotides of the repeats making up the packaging sequence. Forms a
CC transcription factor called DEF-A through cooperative binding with
CC packaging protein 1 (Probable). DEF-A binds to downstream elements of
CC the major late promoter (MLP) and stimulates transcription from the MLP
CC after initiation of viral DNA replication. Simultaneously suppresses
CC early gene expression and is thus likely to participate in the early-
CC late switch in the expression pattern of the late viral proteins. May
CC as well enhance transcription from IVa2 and pIX promoters.
CC {ECO:0000269|PubMed:16809303, ECO:0000269|PubMed:19176628,
CC ECO:0000269|PubMed:20621673, ECO:0000269|PubMed:22811519, ECO:0000305}.
CC -!- SUBUNIT: Part of a genome packaging complex composed of packaging
CC proteins 1, 2 and 3; this complex specifically binds to the packaging
CC sequence on the left end of viral genomic DNA and performs packaging of
CC the viral genome. Self-assembles into higher-order structures.
CC {ECO:0000269|PubMed:17804492, ECO:0000269|PubMed:20925313,
CC ECO:0000269|PubMed:22811519}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:22944109}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Packaging protein 2; Synonyms=Packaging protein 22K, L4-22K;
CC IsoId=P0DJX1-1; Sequence=Displayed;
CC Name=Protein 33K; Synonyms=Splicing factor 33K, L4-33K;
CC IsoId=P24939-1; Sequence=External;
CC -!- INDUCTION: Expressed at the late phase of the viral replicative cycle.
CC Probably already expressed from the early to late transition.
CC -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC are produced by alternative splicing and alternative polyadenylation of
CC the same gene giving rise to non-overlapping ORFs. Expression of
CC packaging protein 2 and splicing factor is controlled by a L4 promoter
CC distinct from the major late promoter.
CC -!- MISCELLANEOUS: [Isoform Packaging protein 2]: Unspliced isoform.
CC -!- SIMILARITY: Belongs to the adenoviridae splicing factor family.
CC {ECO:0000305}.
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DR EMBL; J01917; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; AP_000180.1; AC_000007.1. [P0DJX1-1]
DR Proteomes; UP000008167; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0039708; P:nuclear capsid assembly; IDA:UniProtKB.
DR GO; GO:0019073; P:viral DNA genome packaging; IEA:InterPro.
DR InterPro; IPR021304; Adeno_L4-33K/L4-22K.
DR Pfam; PF11081; DUF2890; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Host nucleus; Host-virus interaction;
KW Late protein; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Viral genome packaging;
KW Viral release from host cell.
FT CHAIN 1..195
FT /note="Packaging protein 2"
FT /id="PRO_0000421107"
FT REGION 1..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..51
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 195 AA; 21543 MW; 0FF447098C8460AA CRC64;
MAPKKKLQLP PPPPTDEEEY WDSQAEEVLD EEEEMMEDWD SLDEASEAEE VSDETPSPSV
AFPSPAPQKL ATVPSIATTS APQAPPALPV RRPNRRWDTT GTRAGKSKQP PPLAQEQQQR
QGYRSWRGHK NAIVACLQDC GGNISFARRF LLYHHGVAFP RNILHYYRHL YSPYCTGGSG
SGSNSSGHTE AKATG
