PKG2_ADE05
ID PKG2_ADE05 Reviewed; 196 AA.
AC Q2KS03;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2013, sequence version 2.
DT 29-SEP-2021, entry version 41.
DE RecName: Full=Packaging protein 2;
DE AltName: Full=Packaging protein 22K;
DE Short=L4-22K;
GN ORFNames=L4;
OS Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus.
OX NCBI_TaxID=28285;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1727603; DOI=10.1016/0042-6822(92)90082-z;
RA Chroboczek J., Bieber F., Jacrot B.;
RT "The sequence of the genome of adenovirus type 5 and its comparison with
RT the genome of adenovirus type 2.";
RL Virology 186:280-285(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate NHRC Ad5FS 7151;
RX PubMed=16481660; DOI=10.1101/gr.4337206;
RA Lin B., Wang Z., Vora G.J., Thornton J.A., Schnur J.M., Thach D.C.,
RA Blaney K.M., Ligler A.G., Malanoski A.P., Santiago J., Walter E.A.,
RA Agan B.K., Metzgar D., Seto D., Daum L.T., Kruzelock R., Rowley R.K.,
RA Hanson E.H., Tibbetts C., Stenger D.A.;
RT "Broad-spectrum respiratory tract pathogen identification using
RT resequencing DNA microarrays.";
RL Genome Res. 16:527-535(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=23142869; DOI=10.1038/nmeth.2227;
RA Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.;
RT "De novo derivation of proteomes from transcriptomes for transcript and
RT protein identification.";
RL Nat. Methods 9:1207-1211(2012).
RN [4]
RP FUNCTION.
RX PubMed=16809303; DOI=10.1128/jvi.00123-06;
RA Ostapchuk P., Anderson M.E., Chandrasekhar S., Hearing P.;
RT "The L4 22-kilodalton protein plays a role in packaging of the adenovirus
RT genome.";
RL J. Virol. 80:6973-6981(2006).
RN [5]
RP REVIEW, AND ALTERNATIVE SPLICING.
RX PubMed=18508565; DOI=10.2741/3059;
RA Akusjarvi G.;
RT "Temporal regulation of adenovirus major late alternative RNA splicing.";
RL Front. Biosci. 13:5006-5015(2008).
RN [6]
RP FUNCTION.
RX PubMed=19176628; DOI=10.1128/jvi.02455-08;
RA Morris S.J., Leppard K.N.;
RT "Adenovirus serotype 5 L4-22K and L4-33K proteins have distinct functions
RT in regulating late gene expression.";
RL J. Virol. 83:3049-3058(2009).
RN [7]
RP FUNCTION, AND IDENTIFICATION IN THE PACKAGING COMPLEX.
RX PubMed=22811519; DOI=10.1128/jvi.01463-12;
RA Wu K., Orozco D., Hearing P.;
RT "The adenovirus L4-22K Protein is multifunctional and is an integral
RT component of crucial aspects of infection.";
RL J. Virol. 86:10474-10483(2012).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=22944109; DOI=10.1016/j.virol.2012.08.021;
RA Ostberg S., Tormanen Persson H., Akusjarvi G.;
RT "Serine 192 in the tiny RS repeat of the adenoviral L4-33K splicing
RT enhancer protein is essential for function and reorganization of the
RT protein to the periphery of viral replication centers.";
RL Virology 433:273-281(2012).
CC -!- FUNCTION: Component of the packaging machinery which encapsidates the
CC viral DNA into preformed capsids and transcriptional activator of the
CC viral major late promoter (MLP). Binds, along with packaging proteins 1
CC and 3, to the specific packaging sequence on the left end of viral
CC genomic DNA and plays an active role in packaging of the viral genome
CC into preformed capsids. Specifically binds to the 5'-TTTG-3'
CC nucleotides of the repeats making up the packaging sequence. Forms a
CC transcription factor called DEF-A through cooperative binding with
CC packaging protein 1 (Probable). DEF-A binds to downstream elements of
CC the major late promoter (MLP) and stimulates transcription from the MLP
CC after initiation of viral DNA replication. Simultaneously suppresses
CC early gene expression and is thus likely to participate in the early-
CC late switch in the expression pattern of the late viral proteins. May
CC as well enhance transcription from IVa2 and pIX promoters.
CC {ECO:0000269|PubMed:16809303, ECO:0000269|PubMed:19176628,
CC ECO:0000269|PubMed:22811519, ECO:0000305}.
CC -!- SUBUNIT: Part of a genome packaging complex composed of packaging
CC proteins 1, 2 and 3; this complex specifically binds to the packaging
CC sequence on the left end of viral genomic DNA and performs packaging of
CC the viral genome. Self-assembles into higher-order structures.
CC {ECO:0000269|PubMed:22811519}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:22944109}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Packaging protein 2; Synonyms=Packaging protein 22K, L4-22K;
CC IsoId=Q2KS03-1; Sequence=Displayed;
CC Name=Protein 33K; Synonyms=Splicing factor 33K, L4-33K;
CC IsoId=P24940-1; Sequence=External;
CC -!- INDUCTION: Expressed at the late phase of the viral replicative cycle.
CC Probably already expressed from the early to late transition.
CC -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC are produced by alternative splicing and alternative polyadenylation of
CC the same gene giving rise to non-overlapping ORFs. Expression of
CC packaging protein 2 and splicing factor is controlled by a L4 promoter
CC distinct from the major late promoter.
CC -!- MISCELLANEOUS: [Isoform Packaging protein 2]: Unspliced isoform.
CC -!- SIMILARITY: Belongs to the adenoviridae splicing factor family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M73260; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY601635; AAW65518.1; -; Genomic_DNA.
DR RefSeq; AP_000216.1; AC_000008.1. [Q2KS03-1]
DR Proteomes; UP000004992; Genome.
DR Proteomes; UP000125273; Genome.
DR GO; GO:0044815; C:DNA packaging complex; IDA:UniProtKB.
DR GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR GO; GO:0019073; P:viral DNA genome packaging; IMP:UniProtKB.
DR InterPro; IPR021304; Adeno_L4-33K/L4-22K.
DR Pfam; PF11081; DUF2890; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Host nucleus; Host-virus interaction;
KW Late protein; Repressor; Transcription; Transcription regulation;
KW Viral genome packaging; Viral release from host cell.
FT CHAIN 1..196
FT /note="Packaging protein 2"
FT /id="PRO_0000421140"
FT REGION 1..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..52
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 182..183
FT /note="Missing (in strain: Isolate NHRC Ad5FS 7151)"
SQ SEQUENCE 196 AA; 21672 MW; 74968433D2E16F13 CRC64;
MAPKKKLQLP PPPTDEEEYW DSQAEEVLDE EEEDMMEDWE SLDEEASEVE EVSDETPSPS
VAFPSPAPQK SATGSSMATT SAPQAPPALP VRRPNRRWDT TGTRAGKSKQ PPPLAQEQQQ
RQGYRSWRGH KNAIVACLQD CGGNISFARR FLLYHHGVAF PRNILHYYRH LYSPYCTGGS
GSGSNSSGHT EAKATG
