PKG3_ADE05
ID PKG3_ADE05 Reviewed; 415 AA.
AC P04496;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 2.
DT 23-FEB-2022, entry version 77.
DE RecName: Full=Packaging protein 3 {ECO:0000255|HAMAP-Rule:MF_04058};
DE AltName: Full=L1-52/55 kDa protein {ECO:0000255|HAMAP-Rule:MF_04058};
DE AltName: Full=Packaging protein 52K {ECO:0000255|HAMAP-Rule:MF_04058};
GN Name=L1 {ECO:0000255|HAMAP-Rule:MF_04058};
OS Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5).
OC Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC Rowavirales; Adenoviridae; Mastadenovirus.
OX NCBI_TaxID=28285;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1727603; DOI=10.1016/0042-6822(92)90082-z;
RA Chroboczek J., Bieber F., Jacrot B.;
RT "The sequence of the genome of adenovirus type 5 and its comparison with
RT the genome of adenovirus type 2.";
RL Virology 186:280-285(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=23142869; DOI=10.1038/nmeth.2227;
RA Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.;
RT "De novo derivation of proteomes from transcriptomes for transcript and
RT protein identification.";
RL Nat. Methods 9:1207-1211(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-173.
RX PubMed=6325298; DOI=10.1016/0378-1119(84)90244-0;
RA Dekker B.M.M., van Ormondt H.;
RT "The nucleotide sequence of fragment HindIII-C of human adenovirus type 5
RT DNA (map positions 17.1-31.7).";
RL Gene 27:115-120(1984).
RN [4]
RP FUNCTION, AND INTERACTION WITH PACKAGING PROTEIN 1.
RX PubMed=15681437; DOI=10.1128/jvi.79.4.2366-2374.2005;
RA Perez-Romero P., Tyler R.E., Abend J.R., Dus M., Imperiale M.J.;
RT "Analysis of the interaction of the adenovirus L1 52/55-kilodalton and IVa2
RT proteins with the packaging sequence in vivo and in vitro.";
RL J. Virol. 79:2366-2374(2005).
RN [5]
RP INTERACTION WITH HEXON-LINKING PROTEIN IIIA PROTEIN.
RX PubMed=21632753; DOI=10.1128/jvi.00467-11;
RA Ma H.C., Hearing P.;
RT "Adenovirus structural protein IIIa is involved in the serotype specificity
RT of viral DNA packaging.";
RL J. Virol. 85:7849-7855(2011).
RN [6]
RP IDENTIFICATION IN THE GENOME PACKAGING COMPLEX.
RX PubMed=22811519; DOI=10.1128/jvi.01463-12;
RA Wu K., Orozco D., Hearing P.;
RT "The adenovirus L4-22K Protein is multifunctional and is an integral
RT component of crucial aspects of infection.";
RL J. Virol. 86:10474-10483(2012).
CC -!- FUNCTION: Involved in viral genome packaging through its interaction
CC with packaging proteins 1 and 2. After proteolytic cleavage by
CC adenovirus protease, L1 52/55k protein is removed from the capsid
CC during viral maturation. {ECO:0000255|HAMAP-Rule:MF_04058,
CC ECO:0000269|PubMed:15681437}.
CC -!- SUBUNIT: Part of the genome packaging complex composed of packaging
CC proteins 1, 2 and 3; this complex specifically binds to the packaging
CC sequence on the left end of viral genomic DNA and performs packaging of
CC the viral genome (PubMed:22811519). Interacts with hexon-linking
CC protein IIIa; this interaction is required to promote correct genome
CC packaging (PubMed:21632753). {ECO:0000255|HAMAP-Rule:MF_04058,
CC ECO:0000269|PubMed:15681437, ECO:0000269|PubMed:21632753,
CC ECO:0000269|PubMed:22811519}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04058}.
CC Note=Nuclear protein present in empty capsids and assembly
CC intermediates. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC -!- INDUCTION: Expressed in the early phase and late phase of the viral
CC replicative cycle. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC -!- PTM: Cleaved at different sites by the viral protease during virion
CC maturation. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC are produced by alternative splicing and alternative polyadenylation of
CC the same gene giving rise to non-overlapping ORFs. A leader sequence is
CC present in the N-terminus of all these mRNAs and is recognized by the
CC viral shutoff protein to provide expression although conventional
CC translation via ribosome scanning from the cap has been shut off in the
CC host cell. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC -!- SIMILARITY: Belongs to the adenoviridae packaging protein 3 family.
CC {ECO:0000255|HAMAP-Rule:MF_04058}.
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DR EMBL; M73260; AAA96406.1; -; Genomic_DNA.
DR EMBL; X02996; CAA26751.1; -; Genomic_DNA.
DR PIR; G39449; WMAD65.
DR RefSeq; AP_000204.1; AC_000008.1.
DR Proteomes; UP000004992; Genome.
DR GO; GO:0044815; C:DNA packaging complex; IDA:UniProtKB.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019073; P:viral DNA genome packaging; IEA:UniProtKB-UniRule.
DR GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04058; ADV_PKG3; 1.
DR InterPro; IPR037536; ADV_PKG3.
DR InterPro; IPR004292; L1-like.
DR Pfam; PF03052; Adeno_52K; 1.
PE 1: Evidence at protein level;
KW Host nucleus; Late protein; Phosphoprotein; Viral genome packaging;
KW Viral release from host cell.
FT CHAIN 1..415
FT /note="Packaging protein 3"
FT /id="PRO_0000221866"
FT REGION 1..173
FT /note="Interaction with packaging protein 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..415
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 351..352
FT /note="Cleavage; by viral protease"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
FT MOD_RES 75
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
FT MOD_RES 360
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
FT CONFLICT 79
FT /note="Y -> H (in Ref. 2; no nucleotide entry)"
SQ SEQUENCE 415 AA; 47060 MW; DA51B34066BA0129 CRC64;
MHPVLRQMRP PPQQRQEQEQ RQTCRAPSPP PTASGGATSA VDAAADGDYE PPRRRARHYL
DLEEGEGLAR LGAPSPERYP RVQLKRDTRE AYVPRQNLFR DREGEEPEEM RDRKFHAGRE
LRHGLNRERL LREEDFEPDA RTGISPARAH VAAADLVTAY EQTVNQEINF QKSFNNHVRT
LVAREEVAIG LMHLWDFVSA LEQNPNSKPL MAQLFLIVQH SRDNEAFRDA LLNIVEPEGR
WLLDLINILQ SIVVQERSLS LADKVAAINY SMLSLGKFYA RKIYHTPYVP IDKEVKIEGF
YMRMALKVLT LSDDLGVYRN ERIHKAVSVS RRRELSDREL MHSLQRALAG TGSGDREAES
YFDAGADLRW APSRRALEAA GAGPGLAVAP ARAGNVGGVE EYDEDDEYEP EDGEY
