PKHA4_MOUSE
ID PKHA4_MOUSE Reviewed; 588 AA.
AC Q8VC98; Q8R3N3;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Pleckstrin homology domain-containing family A member 4;
DE Short=PH domain-containing family A member 4;
DE AltName: Full=Phosphoinositol 3-phosphate-binding protein 1;
DE Short=PEPP-1;
GN Name=Plekha4; Synonyms=Pepp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Binds specifically to phosphatidylinositol 3-phosphate
CC (PtdIns3P), but not to other phosphoinositides. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VC98-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VC98-2; Sequence=VSP_009773, VSP_009774;
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DR EMBL; BC021387; AAH21387.1; -; mRNA.
DR EMBL; BC024961; AAH24961.1; -; mRNA.
DR AlphaFoldDB; Q8VC98; -.
DR SMR; Q8VC98; -.
DR STRING; 10090.ENSMUSP00000051468; -.
DR iPTMnet; Q8VC98; -.
DR PhosphoSitePlus; Q8VC98; -.
DR jPOST; Q8VC98; -.
DR PaxDb; Q8VC98; -.
DR PRIDE; Q8VC98; -.
DR ProteomicsDB; 289900; -. [Q8VC98-1]
DR ProteomicsDB; 289901; -. [Q8VC98-2]
DR MGI; MGI:1916467; Plekha4.
DR eggNOG; ENOG502RI1J; Eukaryota.
DR InParanoid; Q8VC98; -.
DR Reactome; R-MMU-1660499; Synthesis of PIPs at the plasma membrane.
DR ChiTaRS; Plekha4; mouse.
DR PRO; PR:Q8VC98; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8VC98; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; ISO:MGI.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; ISO:MGI.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; ISO:MGI.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; ISO:MGI.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISO:MGI.
DR GO; GO:2000096; P:positive regulation of Wnt signaling pathway, planar cell polarity pathway; ISO:MGI.
DR CDD; cd13248; PH_PEPP1_2_3; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR040392; PKHA4-7_PH.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Lipid-binding; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..588
FT /note="Pleckstrin homology domain-containing family A
FT member 4"
FT /id="PRO_0000053881"
FT DOMAIN 54..153
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 155..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..588
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..206
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..231
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..253
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..542
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 550..577
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 164
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H4M7"
FT MOD_RES 562
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H4M7"
FT VAR_SEQ 1..122
FT /note="MEQGRPRSSLSLASSASTVSSLSSLSTKKPTRAVHKVHAFGKRNNALRRDPN
FT LPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSVRPDGP
FT GAPRGRRFTFT -> MHFDETPTCRCTSVAGCTNRTAQASGCGSAVGSSFQAIASSTIR
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009773"
FT VAR_SEQ 526..588
FT /note="SLSESLELSSPQSPEVDWSRPSGGDRALSSPQSGVGSPRVSRASSPECRQPS
FT SPLLRTKVDHL -> TLEWNLNRQTWWQVPLSAEISHQPLI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009774"
FT CONFLICT 486
FT /note="H -> Q (in Ref. 1; AAH24961)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 588 AA; 64839 MW; F38C722D5A0280C0 CRC64;
MEQGRPRSSL SLASSASTVS SLSSLSTKKP TRAVHKVHAF GKRNNALRRD PNLPVHIRGW
LHKQDSSGLR LWKRRWFVLS GHCLFYYKDS REESVLGSVL LPSYSVRPDG PGAPRGRRFT
FTAEHPGMRT YVLAADTLED LRGWLRALGR ASRAEGEDCG LPRSPARPRP GEGPGGPGGP
PEVNRREEGR ISESPEVARL SRGLGRHETH TPNSTVDLQT DTWSRRTRSP ELFSPLSRPP
SPLSLPRPRS APARRPPLSA GDISFPARPH TPLSRIDVRP PLDWGPQRQT LSRPPIPRRG
PSSEAGGERP PRSPQPRTPE QRTQSTQATS GSSTYLQLPP RPPGTQASMI LLPGPPVDSA
LHQSLETDTL LTKLCGQDRL LRQLQEDLDK RQEEKEQLEA ALELTRQQLG QATREAAASG
KAWGRQRLLQ DRLVNVRAAL CHLAQERERV WDTYSGLEQD LGTLRETLEY LLHLGSPQDR
ACAQQHLWMV EDTLAGLGGP QKQPPHTDPK SPSPAPQGEE SSERESLSES LELSSPQSPE
VDWSRPSGGD RALSSPQSGV GSPRVSRASS PECRQPSSPL LRTKVDHL
