PKHA6_MOUSE
ID PKHA6_MOUSE Reviewed; 1173 AA.
AC Q7TQG1; Q8K0J5;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Pleckstrin homology domain-containing family A member 6;
DE Short=PH domain-containing family A member 6;
DE AltName: Full=Phosphoinositol 3-phosphate-binding protein 3;
DE Short=PEPP-3;
GN Name=Plekha6; Synonyms=Pepp3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Colon, and Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-868; SER-901 AND SER-992, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-492, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=18034455; DOI=10.1021/pr0701254;
RA Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
RT "Large-scale identification and evolution indexing of tyrosine
RT phosphorylation sites from murine brain.";
RL J. Proteome Res. 7:311-318(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-925, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247; SER-251; SER-314;
RP SER-864; THR-868; SER-901; THR-908; SER-992; THR-1140; SER-1142; THR-1145;
RP SER-1146; SER-1149 AND THR-1151, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC031183; AAH31183.1; -; mRNA.
DR EMBL; BC054547; AAH54547.1; -; mRNA.
DR CCDS; CCDS15292.1; -.
DR RefSeq; NP_891846.1; NM_182930.2.
DR AlphaFoldDB; Q7TQG1; -.
DR BMRB; Q7TQG1; -.
DR SMR; Q7TQG1; -.
DR BioGRID; 232235; 1.
DR IntAct; Q7TQG1; 2.
DR MINT; Q7TQG1; -.
DR STRING; 10090.ENSMUSP00000048214; -.
DR iPTMnet; Q7TQG1; -.
DR PhosphoSitePlus; Q7TQG1; -.
DR MaxQB; Q7TQG1; -.
DR PaxDb; Q7TQG1; -.
DR PRIDE; Q7TQG1; -.
DR ProteomicsDB; 289653; -.
DR Antibodypedia; 34556; 114 antibodies from 20 providers.
DR DNASU; 240753; -.
DR Ensembl; ENSMUST00000038295; ENSMUSP00000048214; ENSMUSG00000041757.
DR GeneID; 240753; -.
DR KEGG; mmu:240753; -.
DR UCSC; uc007cpu.2; mouse.
DR CTD; 22874; -.
DR MGI; MGI:2388662; Plekha6.
DR VEuPathDB; HostDB:ENSMUSG00000041757; -.
DR eggNOG; ENOG502QQHD; Eukaryota.
DR GeneTree; ENSGT00940000159692; -.
DR HOGENOM; CLU_008216_0_0_1; -.
DR InParanoid; Q7TQG1; -.
DR OMA; WHHSGET; -.
DR OrthoDB; 71844at2759; -.
DR PhylomeDB; Q7TQG1; -.
DR TreeFam; TF329090; -.
DR Reactome; R-MMU-1660499; Synthesis of PIPs at the plasma membrane.
DR BioGRID-ORCS; 240753; 2 hits in 60 CRISPR screens.
DR ChiTaRS; Plekha6; mouse.
DR PRO; PR:Q7TQG1; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q7TQG1; protein.
DR Bgee; ENSMUSG00000041757; Expressed in interventricular septum and 169 other tissues.
DR ExpressionAtlas; Q7TQG1; baseline and differential.
DR Genevisible; Q7TQG1; MM.
DR CDD; cd13248; PH_PEPP1_2_3; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR040392; PKHA4-7_PH.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1173
FT /note="Pleckstrin homology domain-containing family A
FT member 6"
FT /id="PRO_0000053885"
FT DOMAIN 59..158
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 163..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 737..872
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 888..984
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1093..1114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1130..1173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..232
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 756..795
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 796..829
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 830..870
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 911..932
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 944..958
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1143..1157
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 247
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 251
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 314
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 459
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 461
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 472
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 492
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:18034455"
FT MOD_RES 665
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 864
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 868
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 901
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 908
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 925
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 973
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 979
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 992
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 1045
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 1065
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2H5"
FT MOD_RES 1140
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1142
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1145
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1146
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1149
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1151
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 1173 AA; 131427 MW; A71B82D513EE8B36 CRC64;
MSNKTGGKRS ATINSDIANH NMVSEVPPER PNIRATRTSR KAIAFGKRAH SMKRNPNAPV
TKAGWLYKQA SSGVKQWNKR WFVLVDRCLF YYKDEKQESI LGSIPLLSFR VAAVQPSDNI
SRKHTFKAEH AGVRTYFFSA ESPEEQEAWI QAMGEAARVQ IPPAQKSVPQ PVRHSLEKPD
SENIPPSKHH QQPPHNNLTK LEPEAKTRGE GDGRGCEKAE RRPERPEVKK ETLVKANGLP
SGPETASEPG SPYPDGPRVP GGGEHPAQPN GWQYSSPSRP GSTAFPPHDG DSGGQRRSFP
PRTDPDKIAQ RKSSMNQLQQ WVNLRRGVPP PEDLRSPSRF YPMPRRVPDY YNPYSSQYPD
DYQYYPPGVR PDSICSMPAY DRISPPWALE DKRHSFRNGG GPTYQLHEWK ESTSYGRQDG
TVWIPSPSRQ PVFYDELDAA SGSLRRLSLQ PRSHSVPRSP SQGSYSRARI YSPVRSPSAR
FDRLPPRSED IYADPAAYVM RRSISSPKYD YLGDRRPVPA GLFPYNYPSS PTVHDKMDEL
LDLQLQRNLE YLDQQMSESE TLISMVNRMV ENSSPRAHLF MQVPAYPEVF RDGLHTFKLN
EQDTDKLLGK LCEQNKVVRE QERLVQQLRA EKESLESALM GTHQELEMFG SQPAYPEKLL
HKKESLQNQL INIRVELSQA TTALTNSTVV YENLESEVSA LHDELWEQLN LDIQNEVLNR
QIQKEIWRIQ DVMEGLRKNN PSRGTDTAKH RGGLGPSATY SSNSPASPLS SASLTSPLSP
FSMVSGSQGS PTKPGSSEEP GPPRPPLPKA YVPLESPPTV PPLPNESRFW PYPNSPSWHR
SGETAKGQPK TGYETSKKDP SQTSPLGTPR DINLVPTRQE VEAEKQAALN KVGIVPPRTK
SPAEEELTPS AVVRRTTNGL TNGLSSRQER PKSAVFSGEG KVKMSVEEQM DRMRRHQSGS
MKEKRRSLQL PASPAPEPST RPAYKVVRRH RSIHEVDISN LEAALRAEEP GGQAYETPRE
EIARLRKMEL EPQHYDVDIS KELSTPDKVL IPERYIDLEP DTPLSPEELK EKQKKVERIK
TLIAKSSMQN VVPIGEGDSV DVPQDSESQL QEQEKRIEIS CALATEASRR GRMLSVQCAT
PSPPTSPASP TPPVNPLSSD RPRGADSSHT MRV
