ID   PKHA8_DANRE             Reviewed;         549 AA.
AC   Q5U3N0;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Pleckstrin homology domain-containing family A member 8;
DE            Short=PH domain-containing family A member 8;
GN   Name=plekha8; ORFNames=zgc:101886;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cargo transport protein that is required for apical transport
CC       from the trans-Golgi network (TGN) to the plasma membrane.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus, trans-
CC       Golgi network membrane {ECO:0000250}. Membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Note=Mainly localized to the
CC       trans-Golgi network (TGN) but also found at the rims of Golgi
CC       cisternae. {ECO:0000250}.
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DR   EMBL; BC085465; AAH85465.1; -; mRNA.
DR   RefSeq; NP_001007375.1; NM_001007374.1.
DR   AlphaFoldDB; Q5U3N0; -.
DR   SMR; Q5U3N0; -.
DR   STRING; 7955.ENSDARP00000059622; -.
DR   PaxDb; Q5U3N0; -.
DR   GeneID; 492502; -.
DR   KEGG; dre:492502; -.
DR   CTD; 84725; -.
DR   ZFIN; ZDB-GENE-041114-69; plekha8.
DR   eggNOG; KOG3221; Eukaryota.
DR   InParanoid; Q5U3N0; -.
DR   OrthoDB; 952122at2759; -.
DR   PhylomeDB; Q5U3N0; -.
DR   Reactome; R-DRE-1660499; Synthesis of PIPs at the plasma membrane.
DR   PRO; PR:Q5U3N0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:1902387; F:ceramide 1-phosphate binding; IBA:GO_Central.
DR   GO; GO:1902388; F:ceramide 1-phosphate transfer activity; IBA:GO_Central.
DR   GO; GO:0035627; P:ceramide transport; IBA:GO_Central.
DR   GO; GO:0035621; P:ER to Golgi ceramide transport; IBA:GO_Central.
DR   GO; GO:0120009; P:intermembrane lipid transfer; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3520.10; -; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR036497; GLTP_sf.
DR   InterPro; IPR014830; Glycolipid_transfer_prot_dom.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   Pfam; PF08718; GLTP; 1.
DR   Pfam; PF00169; PH; 1.
DR   SMART; SM00233; PH; 1.
DR   SUPFAM; SSF110004; SSF110004; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Golgi apparatus; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..549
FT                   /note="Pleckstrin homology domain-containing family A
FT                   member 8"
FT                   /id="PRO_0000306867"
FT   DOMAIN          1..93
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   REGION          180..312
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        180..203
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        216..232
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        240..272
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        273..296
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        297..312
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   549 AA;  61454 MW;  0F2AACD0F4C6E145 CRC64;
     MEGVLYKWTN YISGWQPRWF VLEGGTLSYY DSQEDAWKGC KGSIKISVCE IQVHPSDFTR
     VDLIIPGEQY FYLRAINAAE RQKWLVALGT AKACLTDNRT KREKELQENS EALKTKMSEL
     RLYCDLLLQQ VNKIQESPLA ETSAGSGEGG DETGNLVKST CTTFLKTLEE CMHIASRTFN
     PDLLSRTPPG SPSVATIKPQ KIKSNDPKNL HPGETRKDLI NTSGTSAHES GPDNEPPPSP
     QENISTAHTE SGLMEDQNDL IEPNNGSSSS TQDHEEPVEE QQTDGSTEDA DHSQEEQQEV
     SMSPTQNKQE VQEDIQTDLE LNETQSENKQ EEEDEDKVDT FFSTMSHRFS DIILEEDSGI
     PTQAFLDSCY AIVPVLDKLG PTVFAPVKID FVGNIKKIQQ KVVSDPESFP TLQSIVLHEV
     KTEVAQVRNS ATEALLWLKR GLKFLKEFLS EINTGVKDVQ GALYNAYGKT LRQYHGWVVR
     GVFALALRAA PSYEGFMAAL VSYEGDELKE GFRTGMHRDL DIYLPAMENQ LSILDTLYEE
     YGLESDEIV