PKHH1_MOUSE
ID PKHH1_MOUSE Reviewed; 1356 AA.
AC Q80TI1; Q3UPN4; Q8BWF3; Q8K334; Q99JL2;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Pleckstrin homology domain-containing family H member 1;
DE Short=PH domain-containing family H member 1;
GN Name=Plekhh1; Synonyms=Kiaa1200;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Kidney, and Ovary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 726-1356.
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451 AND SER-739, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80TI1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80TI1-2; Sequence=VSP_029350, VSP_029351;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65746.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK122464; BAC65746.1; ALT_INIT; mRNA.
DR EMBL; AK052692; BAC35099.1; -; mRNA.
DR EMBL; AK143387; BAE25361.1; -; mRNA.
DR EMBL; BC006045; AAH06045.1; -; mRNA.
DR EMBL; BC028900; AAH28900.1; -; mRNA.
DR CCDS; CCDS49097.1; -. [Q80TI1-1]
DR RefSeq; NP_851418.2; NM_181073.3. [Q80TI1-1]
DR RefSeq; XP_006515708.1; XM_006515645.2. [Q80TI1-1]
DR AlphaFoldDB; Q80TI1; -.
DR SMR; Q80TI1; -.
DR BioGRID; 229275; 1.
DR STRING; 10090.ENSMUSP00000049460; -.
DR iPTMnet; Q80TI1; -.
DR PhosphoSitePlus; Q80TI1; -.
DR MaxQB; Q80TI1; -.
DR PaxDb; Q80TI1; -.
DR PeptideAtlas; Q80TI1; -.
DR PRIDE; Q80TI1; -.
DR ProteomicsDB; 289604; -. [Q80TI1-1]
DR ProteomicsDB; 289605; -. [Q80TI1-2]
DR Antibodypedia; 64198; 74 antibodies from 17 providers.
DR DNASU; 211945; -.
DR Ensembl; ENSMUST00000039928; ENSMUSP00000049460; ENSMUSG00000060716. [Q80TI1-1]
DR Ensembl; ENSMUST00000219956; ENSMUSP00000151747; ENSMUSG00000060716. [Q80TI1-1]
DR GeneID; 211945; -.
DR KEGG; mmu:211945; -.
DR UCSC; uc007nzr.2; mouse. [Q80TI1-1]
DR CTD; 57475; -.
DR MGI; MGI:2144989; Plekhh1.
DR VEuPathDB; HostDB:ENSMUSG00000060716; -.
DR eggNOG; KOG0248; Eukaryota.
DR GeneTree; ENSGT00940000159456; -.
DR HOGENOM; CLU_001626_3_1_1; -.
DR InParanoid; Q80TI1; -.
DR OMA; NRKASMP; -.
DR OrthoDB; 75793at2759; -.
DR PhylomeDB; Q80TI1; -.
DR TreeFam; TF312866; -.
DR BioGRID-ORCS; 211945; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Plekhh1; mouse.
DR PRO; PR:Q80TI1; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q80TI1; protein.
DR Bgee; ENSMUSG00000060716; Expressed in paneth cell and 225 other tissues.
DR ExpressionAtlas; Q80TI1; baseline and differential.
DR Genevisible; Q80TI1; MM.
DR GO; GO:0005856; C:cytoskeleton; IEA:InterPro.
DR CDD; cd14473; FERM_B-lobe; 1.
DR Gene3D; 1.20.80.10; -; 1.
DR Gene3D; 1.25.40.530; -; 1.
DR Gene3D; 2.30.29.30; -; 3.
DR InterPro; IPR019749; Band_41_domain.
DR InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf.
DR InterPro; IPR035963; FERM_2.
DR InterPro; IPR019748; FERM_central.
DR InterPro; IPR000299; FERM_domain.
DR InterPro; IPR000857; MyTH4_dom.
DR InterPro; IPR038185; MyTH4_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR040105; PKHH1.
DR PANTHER; PTHR22903:SF4; PTHR22903:SF4; 1.
DR Pfam; PF00373; FERM_M; 1.
DR Pfam; PF00784; MyTH4; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00295; B41; 1.
DR SMART; SM00139; MyTH4; 1.
DR SMART; SM00233; PH; 2.
DR SUPFAM; SSF47031; SSF47031; 1.
DR PROSITE; PS50057; FERM_3; 1.
DR PROSITE; PS51016; MYTH4; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome;
KW Repeat.
FT CHAIN 1..1356
FT /note="Pleckstrin homology domain-containing family H
FT member 1"
FT /id="PRO_0000310949"
FT DOMAIN 572..666
FT /note="PH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 681..790
FT /note="PH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 826..980
FT /note="MyTH4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00359"
FT DOMAIN 991..1327
FT /note="FERM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00084"
FT REGION 179..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 27..172
FT /evidence="ECO:0000255"
FT COILED 359..407
FT /evidence="ECO:0000255"
FT COMPBIAS 279..304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..408
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 451
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 739
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..681
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029350"
FT VAR_SEQ 682..690
FT /note="PTVKGWLTK -> MCVCSCCSL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029351"
FT CONFLICT 93
FT /note="R -> K (in Ref. 1; BAC65746)"
FT /evidence="ECO:0000305"
FT CONFLICT 726
FT /note="Q -> E (in Ref. 3; AAH28900)"
FT /evidence="ECO:0000305"
FT CONFLICT 800..803
FT /note="YEQL -> HASV (in Ref. 3; AAH06045)"
FT /evidence="ECO:0000305"
FT CONFLICT 1016
FT /note="F -> L (in Ref. 3; AAH28900)"
FT /evidence="ECO:0000305"
FT CONFLICT 1156
FT /note="T -> A (in Ref. 1; BAC65746 and 3; AAH06045/
FT AAH28900)"
FT /evidence="ECO:0000305"
FT CONFLICT 1159..1172
FT /note="QHLLQQVLDRFYPR -> PRPGWTVLCFCFLH (in Ref. 3;
FT AAH06045)"
FT /evidence="ECO:0000305"
FT CONFLICT 1257
FT /note="Missing (in Ref. 3; AAH28900)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1356 AA; 150909 MW; 023328737EF57F44 CRC64;
MAEVKVEVAS IDWQKRCLSL ETQLFRFRLQ ASKIRELLAD KMQELEQRLL EAEQRAENAE
TQVGVMEEKI KLSNLKSVDS TGTLHQKYQE LLRAVQGKDE LISQLQAQLE KQKQTRAEEA
KIVQEKAAKI KEWVTVKLAE LEMENQQLKT CNQQLVEQVA ALQDALEDLR MTPSEELLVV
PEGTPERDPV PSGPSDQPVE QDSNPHTQIL KVAVPTPSLG TLQSRDSLSE ARSLEDLRFS
MVHPGETAEA KTLQSHLQKE GSPSQLCMKP GNPKHGSASY RESLVTAQGG TFPGTKTSAR
EGGPGSSLTL PKVRAPSIPR DSFQVAKRHH SQPQVGPGHC DHVVSIEIGA LSALHSPGSS
KSEARAKVRE EAEKMEMEAL PPSGKQEERE SLKSRRGELE DVELENKPPT PPLHRFPSWE
SRIYAMATSG MQLSEVSSRR SNAACYASGP SALAFPGAFS GLVYKNVTVP VYTALKGKAT
QISNVPFVDE SSGSDDDCGS QASFRMSVPC SEYRKTSGLG SPRAIKRGVS MSSLSSEGDY
AIPPDACSLD SDYSEPEHKL QRTSSYSTDG EALEKSGYLL KMGSRVKTWK RRWFVLRQGQ
ILYYKSPSDV IRKPQGQVDL NSHCQIVREE EAQTFQLISG NKTYYLTAES PSLLEEWIRV
LQSLLKVQVT GPPALHQGGT KPTVKGWLTK VKHGHSKLVW CALVGKTFYY YRSHEDKRPL
GCLPVQDAHI EEVDRSCDSD EDYEAGGTGR LLSSHCTLVI HPPEHSPTYL LIGTKHEKDT
WLYHLTVAAG GSNAKVGTVY EQLIGKLMDG EGNPDSPLWR HPMLCYSQEG LCASLTTLPS
EALQTEALKL FKSCQLFINV PVEAASVDYH VSLAQTALQV CLVHPELQSE IYCQLMKQIS
CRPPQKYSLM QCWQLLALCA PLFLPQHHFL WYVKQQLQRH ADPRSETGQY AIYCQRAVER
TLQTGEREAR PSRMEVVSIL LRNPFHHSLP FSIPVHFANG TYQVVGFDGS STVDEFLQRL
NQETGMRKPS QSGFALFTDD PSGRDLEHCL QGRVKICDAI SKWEQTLKEL HPGKSEGGTR
VVKLMYKNRL YFRSQVKGET ERERLLLAFQ ASGEIVAGRF PVTKELALEM AALMAQVEYG
DLEKPTLPGP GGTPPTKAQH LLQQVLDRFY PRRYRNGAPP EQLRHLADMM ATKWAALQGC
SPPECIRIYL TVARKWPLFG AKLFAAQPAQ LSPKENTVVW IAVNEDGVSI LDHRTMQVNI
TYPYSSVTTF GGCRDDFMLV IRSIPDQSSG KTRIDKLTFR MPAPKITETT LMMASYMNHC
SATVNLSAKL PAARQPRDLD GQFFASVSCT KGSALL
