PKHH3_MOUSE
ID PKHH3_MOUSE Reviewed; 796 AA.
AC Q8VCE9; Q3U3Z6; Q8R1H2;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Pleckstrin homology domain-containing family H member 3;
DE Short=PH domain-containing family H member 3;
DE Flags: Precursor;
GN Name=Plekhh3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 384-796 (ISOFORM 3).
RC STRAIN=FVB/N; TISSUE=Colon, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8VCE9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VCE9-2; Sequence=VSP_029399;
CC Name=3;
CC IsoId=Q8VCE9-3; Sequence=VSP_029399, VSP_029400, VSP_029401;
CC Name=4;
CC IsoId=Q8VCE9-4; Sequence=VSP_029400, VSP_029401;
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DR EMBL; AK154510; BAE32639.1; -; mRNA.
DR EMBL; AL590969; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC020025; AAH20025.1; -; mRNA.
DR EMBL; BC024538; AAH24538.1; -; mRNA.
DR CCDS; CCDS25453.1; -. [Q8VCE9-1]
DR CCDS; CCDS83899.1; -. [Q8VCE9-2]
DR RefSeq; NP_001333698.1; NM_001346769.1. [Q8VCE9-2]
DR RefSeq; NP_666142.1; NM_146030.2. [Q8VCE9-1]
DR AlphaFoldDB; Q8VCE9; -.
DR SMR; Q8VCE9; -.
DR BioGRID; 229859; 1.
DR STRING; 10090.ENSMUSP00000046044; -.
DR GlyGen; Q8VCE9; 1 site.
DR iPTMnet; Q8VCE9; -.
DR PhosphoSitePlus; Q8VCE9; -.
DR MaxQB; Q8VCE9; -.
DR PaxDb; Q8VCE9; -.
DR PRIDE; Q8VCE9; -.
DR ProteomicsDB; 289655; -. [Q8VCE9-1]
DR ProteomicsDB; 289656; -. [Q8VCE9-2]
DR ProteomicsDB; 289657; -. [Q8VCE9-3]
DR ProteomicsDB; 289658; -. [Q8VCE9-4]
DR Antibodypedia; 29328; 33 antibodies from 13 providers.
DR DNASU; 217198; -.
DR Ensembl; ENSMUST00000043397; ENSMUSP00000046044; ENSMUSG00000035172. [Q8VCE9-1]
DR Ensembl; ENSMUST00000129895; ENSMUSP00000137841; ENSMUSG00000035172. [Q8VCE9-4]
DR Ensembl; ENSMUST00000164474; ENSMUSP00000127088; ENSMUSG00000035172. [Q8VCE9-2]
DR GeneID; 217198; -.
DR KEGG; mmu:217198; -.
DR UCSC; uc007lnq.2; mouse. [Q8VCE9-1]
DR UCSC; uc007lnr.2; mouse. [Q8VCE9-2]
DR CTD; 79990; -.
DR MGI; MGI:2384950; Plekhh3.
DR VEuPathDB; HostDB:ENSMUSG00000035172; -.
DR eggNOG; KOG4229; Eukaryota.
DR GeneTree; ENSGT00940000159764; -.
DR HOGENOM; CLU_001626_4_0_1; -.
DR InParanoid; Q8VCE9; -.
DR OMA; QDSKNTF; -.
DR OrthoDB; 478730at2759; -.
DR PhylomeDB; Q8VCE9; -.
DR TreeFam; TF327852; -.
DR BioGRID-ORCS; 217198; 3 hits in 74 CRISPR screens.
DR PRO; PR:Q8VCE9; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8VCE9; protein.
DR Bgee; ENSMUSG00000035172; Expressed in lip and 166 other tissues.
DR ExpressionAtlas; Q8VCE9; baseline and differential.
DR Genevisible; Q8VCE9; MM.
DR GO; GO:0005856; C:cytoskeleton; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR CDD; cd14473; FERM_B-lobe; 1.
DR Gene3D; 1.20.80.10; -; 1.
DR Gene3D; 1.25.40.530; -; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR019749; Band_41_domain.
DR InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf.
DR InterPro; IPR035963; FERM_2.
DR InterPro; IPR019748; FERM_central.
DR InterPro; IPR000299; FERM_domain.
DR InterPro; IPR000857; MyTH4_dom.
DR InterPro; IPR038185; MyTH4_dom_sf.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000159; RA_dom.
DR Pfam; PF00373; FERM_M; 1.
DR Pfam; PF00784; MyTH4; 1.
DR Pfam; PF00788; RA; 1.
DR SMART; SM00295; B41; 1.
DR SMART; SM00139; MyTH4; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF47031; SSF47031; 1.
DR PROSITE; PS50057; FERM_3; 1.
DR PROSITE; PS51016; MYTH4; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Methylation; Phosphoprotein;
KW Reference proteome; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..796
FT /note="Pleckstrin homology domain-containing family H
FT member 3"
FT /id="PRO_0000311109"
FT DOMAIN 95..199
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 237..399
FT /note="MyTH4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00359"
FT DOMAIN 404..757
FT /note="FERM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00084"
FT REGION 28..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 557..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 598..625
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 750..796
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..581
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..623
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 754..796
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 30
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z736"
FT MOD_RES 638
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z736"
FT CARBOHYD 474
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 471..474
FT /note="RFEN -> S (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029399"
FT VAR_SEQ 664..705
FT /note="YDVLELSTEPGGGAPQKLCLGLGAKAMSLSRPGETEPIHSVS -> SLVEVL
FT HRSSAWVWEQRPCRSPGLVRQNPSTVSAMVMWPPAS (in isoform 3 and
FT isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029400"
FT VAR_SEQ 706..796
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029401"
SQ SEQUENCE 796 AA; 85842 MW; 343C40578FBC0F24 CRC64;
MPLPGGLWWL LCCRRGFTLL HRDYGDGELS GDGDEDEDDE TFELRTPSPA GGGRGSLDVT
LTQPTRNGPI SDRLQGWEET WSLIPDKGLP EEDPDIIVKG WLYREPRGGG ARPWLLPRRA
WFVLTRDSLD QFSSSGKGAR RLGSLVLTSL CSVTGPERRP KETGLWSVTV SGRKHSIRLC
SPRQAEAERW GVALREVIAS KAPLETPTQL LLRDIQESCG DPEAVALIYR RNPILRHTSS
ALYAPLLPLP YEVSAPGPGY APLREEAVRL FLALQALEGA RRPGPLMQGV LQTCRDLPAL
QDELFLQLAK QTSGPAGPPG LPATQDPATL RYWQLLTCMS CTFRPGGAVR GHLLGHLERT
EQALPDSELA EYARFIRKAL GRTRGRELVP SLAEISALSR RQELLCTVHC PGAGACPVSI
DSHTTAGEVA RELVGRLGLA RSRNAFALYE QRGAQERALA GGTLVADVLT RFENLTSEEA
GLEDSPDCGW RLCLRLHGPL HPEGLSPEGH ELPFLFEQAH ALLLRGRPPP PEDTLRALAA
LRLQSLHRDF SPRGPLPLLD RLLPPPIPPR EQPPCPTRRP PPSAALLAGA LWSPGLAKRR
AERARRGGTG RSTGSTAQVG GGGASTTAAV LGGWKRLRGM GQAEAMAAYL ALAAQCPGFG
AARYDVLELS TEPGGGAPQK LCLGLGAKAM SLSRPGETEP IHSVSYGHVA ACQLIGPHTL
ALRVGDSQLL LQSPQVEEIM ELVNAYLANP SPERPCRSGS SSGPPSQDLP DTSPPSQHQV
LEEPQGQSGC LKQLQD
