PKHO2_HUMAN
ID PKHO2_HUMAN Reviewed; 490 AA.
AC Q8TD55; Q7L4H4; Q8WYS8;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Pleckstrin homology domain-containing family O member 2;
DE Short=PH domain-containing family O member 2;
DE AltName: Full=Pleckstrin homology domain-containing family Q member 1;
DE Short=PH domain-containing family Q member 1;
GN Name=PLEKHO2; Synonyms=PLEKHQ1; ORFNames=PP9099;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Li N., Wan T., Zhang W., Cao X.;
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-490, AND VARIANT SER-290.
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-311; SER-390 AND SER-468, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164; SER-167; SER-273;
RP THR-311 AND SER-390, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- INTERACTION:
CC Q8TD55-2; P24863: CCNC; NbExp=3; IntAct=EBI-12905986, EBI-395261;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8TD55-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TD55-2; Sequence=VSP_029212;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL55880.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF494534; AAM18053.1; -; mRNA.
DR EMBL; AC069368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471082; EAW77697.1; -; Genomic_DNA.
DR EMBL; CH471082; EAW77699.1; -; Genomic_DNA.
DR EMBL; BC008744; AAH08744.2; -; mRNA.
DR EMBL; AF318373; AAL55880.1; ALT_INIT; mRNA.
DR CCDS; CCDS10196.1; -. [Q8TD55-1]
DR CCDS; CCDS73739.1; -. [Q8TD55-2]
DR RefSeq; NP_001181988.1; NM_001195059.1. [Q8TD55-2]
DR RefSeq; NP_079477.2; NM_025201.4. [Q8TD55-1]
DR AlphaFoldDB; Q8TD55; -.
DR SMR; Q8TD55; -.
DR BioGRID; 123215; 35.
DR IntAct; Q8TD55; 19.
DR MINT; Q8TD55; -.
DR STRING; 9606.ENSP00000326706; -.
DR CarbonylDB; Q8TD55; -.
DR GlyGen; Q8TD55; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8TD55; -.
DR PhosphoSitePlus; Q8TD55; -.
DR BioMuta; PLEKHO2; -.
DR DMDM; 74739384; -.
DR EPD; Q8TD55; -.
DR jPOST; Q8TD55; -.
DR MassIVE; Q8TD55; -.
DR MaxQB; Q8TD55; -.
DR PaxDb; Q8TD55; -.
DR PeptideAtlas; Q8TD55; -.
DR PRIDE; Q8TD55; -.
DR ProteomicsDB; 74242; -. [Q8TD55-1]
DR ProteomicsDB; 74243; -. [Q8TD55-2]
DR Antibodypedia; 34926; 108 antibodies from 23 providers.
DR DNASU; 80301; -.
DR Ensembl; ENST00000323544.5; ENSP00000326706.4; ENSG00000241839.10. [Q8TD55-1]
DR Ensembl; ENST00000616065.4; ENSP00000483505.1; ENSG00000241839.10. [Q8TD55-2]
DR GeneID; 80301; -.
DR KEGG; hsa:80301; -.
DR MANE-Select; ENST00000323544.5; ENSP00000326706.4; NM_025201.5; NP_079477.2.
DR UCSC; uc002anv.4; human. [Q8TD55-1]
DR CTD; 80301; -.
DR GeneCards; PLEKHO2; -.
DR HGNC; HGNC:30026; PLEKHO2.
DR HPA; ENSG00000241839; Tissue enhanced (lymphoid).
DR neXtProt; NX_Q8TD55; -.
DR OpenTargets; ENSG00000241839; -.
DR PharmGKB; PA162399756; -.
DR VEuPathDB; HostDB:ENSG00000241839; -.
DR eggNOG; ENOG502QVFF; Eukaryota.
DR GeneTree; ENSGT00530000063760; -.
DR HOGENOM; CLU_026551_3_0_1; -.
DR InParanoid; Q8TD55; -.
DR OMA; MEQDTKE; -.
DR OrthoDB; 827145at2759; -.
DR PhylomeDB; Q8TD55; -.
DR TreeFam; TF333115; -.
DR PathwayCommons; Q8TD55; -.
DR Reactome; R-HSA-6798695; Neutrophil degranulation.
DR SignaLink; Q8TD55; -.
DR BioGRID-ORCS; 80301; 8 hits in 1073 CRISPR screens.
DR ChiTaRS; PLEKHO2; human.
DR GenomeRNAi; 80301; -.
DR Pharos; Q8TD55; Tdark.
DR PRO; PR:Q8TD55; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q8TD55; protein.
DR Bgee; ENSG00000241839; Expressed in spleen and 153 other tissues.
DR Genevisible; Q8TD55; HS.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:1904813; C:ficolin-1-rich granule lumen; TAS:Reactome.
DR GO; GO:0071888; P:macrophage apoptotic process; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR043448; PKHO1/2.
DR PANTHER; PTHR15871; PTHR15871; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..490
FT /note="Pleckstrin homology domain-containing family O
FT member 2"
FT /id="PRO_0000309483"
FT DOMAIN 18..119
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 173..402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 439..481
FT /evidence="ECO:0000255"
FT MOD_RES 164
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 167
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 232
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8K124"
FT MOD_RES 235
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K124"
FT MOD_RES 237
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K124"
FT MOD_RES 238
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K124"
FT MOD_RES 273
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 298
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8K124"
FT MOD_RES 311
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163,
FT ECO:0007744|PubMed:24275569"
FT MOD_RES 390
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163,
FT ECO:0007744|PubMed:24275569"
FT MOD_RES 468
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 4..53
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029212"
FT VARIANT 290
FT /note="P -> S (in dbSNP:rs2010875)"
FT /evidence="ECO:0000269|PubMed:15498874"
FT /id="VAR_036964"
SQ SEQUENCE 490 AA; 53350 MW; 02099F6ECAFA8BF3 CRC64;
MEEEGVKEAG EKPRGAQMVD KAGWIKKSSG GLLGFWKDRY LLLCQAQLLV YENEDDQKCV
ETVELGSYEK CQDLRALLKR KHRFILLRSP GNKVSDIKFQ APTGEEKESW IKALNEGINR
GKNKAFDEVK VDKSCALEHV TRDRVRGGQR RRPPTRVHLK EVASAASDGL LRLDLDVPDS
GPPVFAPSNH VSEAQPRETP RPLMPPTKPF LAPETTSPGD RVETPVGERA PTPVSASSEV
SPESQEDSET PAEEDSGSEQ PPNSVLPDKL KVSWENPSPQ EAPAAESAEP SQAPCSETSE
AAPREGGKPP TPPPKILSEK LKASMGEMQA SGPPAPGTVQ VSVNGMDDSP EPAKPSQAEG
TPGTPPKDAT TSTALPPWDL PPQFHPRCSS LGDLLGEGPR HPLQPRERLY RAQLEVKVAS
EQTEKLLNKV LGSEPAPVSA ETLLSQAVEQ LRQATQVLQE MRDLGELSQE APGLREKRKE
LVTLYRRSAP
