PKHO2_MOUSE
ID PKHO2_MOUSE Reviewed; 495 AA.
AC Q8K124; Q3TCY2; Q3U4R2; Q8C1F2; Q8R140;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Pleckstrin homology domain-containing family O member 2;
DE Short=PH domain-containing family O member 2;
DE AltName: Full=Pleckstrin homology domain-containing family Q member 1;
DE Short=PH domain-containing family Q member 1;
GN Name=Plekho2; Synonyms=Plekhq1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-233; SER-236 AND SER-395, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=19131326; DOI=10.1074/mcp.m800451-mcp200;
RA Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
RT "Large scale localization of protein phosphorylation by use of electron
RT capture dissociation mass spectrometry.";
RL Mol. Cell. Proteomics 8:904-912(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-233; SER-238; SER-239;
RP SER-292 AND THR-296, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK028069; BAC25735.1; -; mRNA.
DR EMBL; AK154092; BAE32369.1; -; mRNA.
DR EMBL; AK170479; BAE41823.1; -; mRNA.
DR EMBL; BC025598; AAH25598.1; -; mRNA.
DR EMBL; BC028908; AAH28908.1; -; mRNA.
DR CCDS; CCDS23294.1; -.
DR RefSeq; NP_694759.1; NM_153119.3.
DR AlphaFoldDB; Q8K124; -.
DR SMR; Q8K124; -.
DR BioGRID; 221902; 1.
DR IntAct; Q8K124; 1.
DR MINT; Q8K124; -.
DR STRING; 10090.ENSMUSP00000063677; -.
DR iPTMnet; Q8K124; -.
DR PhosphoSitePlus; Q8K124; -.
DR EPD; Q8K124; -.
DR jPOST; Q8K124; -.
DR MaxQB; Q8K124; -.
DR PaxDb; Q8K124; -.
DR PeptideAtlas; Q8K124; -.
DR PRIDE; Q8K124; -.
DR ProteomicsDB; 289606; -.
DR Antibodypedia; 34926; 108 antibodies from 23 providers.
DR DNASU; 102595; -.
DR Ensembl; ENSMUST00000068944; ENSMUSP00000063677; ENSMUSG00000050721.
DR GeneID; 102595; -.
DR KEGG; mmu:102595; -.
DR UCSC; uc009qdl.2; mouse.
DR CTD; 80301; -.
DR MGI; MGI:2143132; Plekho2.
DR VEuPathDB; HostDB:ENSMUSG00000050721; -.
DR eggNOG; ENOG502QVFF; Eukaryota.
DR GeneTree; ENSGT00530000063760; -.
DR HOGENOM; CLU_026551_3_0_1; -.
DR InParanoid; Q8K124; -.
DR OMA; MEQDTKE; -.
DR OrthoDB; 827145at2759; -.
DR PhylomeDB; Q8K124; -.
DR TreeFam; TF333115; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 102595; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Plekho2; mouse.
DR PRO; PR:Q8K124; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8K124; protein.
DR Bgee; ENSMUSG00000050721; Expressed in granulocyte and 150 other tissues.
DR Genevisible; Q8K124; MM.
DR GO; GO:0071888; P:macrophage apoptotic process; IMP:MGI.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR043448; PKHO1/2.
DR PANTHER; PTHR15871; PTHR15871; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..495
FT /note="Pleckstrin homology domain-containing family O
FT member 2"
FT /id="PRO_0000309484"
FT DOMAIN 18..120
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 171..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 444..469
FT /evidence="ECO:0000255"
FT COMPBIAS 258..273
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..298
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 326..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TD55"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TD55"
FT MOD_RES 233
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 236
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 238
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 239
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 274
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TD55"
FT MOD_RES 292
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 296
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 395
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19131326,
FT ECO:0007744|PubMed:19144319"
FT CONFLICT 1..4
FT /note="MEEE -> VRRWRNWAEQDKTFP (in Ref. 1; BAE32369)"
FT /evidence="ECO:0000305"
FT CONFLICT 183
FT /note="P -> A (in Ref. 1; BAE41823)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="A -> P (in Ref. 1; BAE32369)"
FT /evidence="ECO:0000305"
FT CONFLICT 330..332
FT /note="GSS -> TRP (in Ref. 2; AAH25598)"
FT /evidence="ECO:0000305"
FT CONFLICT 396..428
FT /note="LGDLLRESPQHPRLPKEKLYRAQLEVKVASKQT -> HAGGVEWGWGLPRLS
FT PCPYDPPFLSFGNKPHFM (in Ref. 1; BAC25735)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 495 AA; 53872 MW; 2105D71A407498FE CRC64;
MEEESIKEGS EKPRGARTAD KAGWIKKSSG GLLGLWKDRY LLLCQAQLLV YENEDEQKCV
ETVELGSYEK CQDLRTLLKR KHHRFILLRS PGNKVSDIKF QAPSGEEKES WIKALNEGIN
RGKNKAFDEV KVDKTCALEH VTRNRVRGGQ RRRPPTRIHL KEVASAASDG LSRLDLDVPD
SGPPVFAPLS DISEDQPQEP PRALMPPVKP SPGPETSAVE DSKETPAGER ALTPDSASSG
ANPESQEDAE TPAKEDSDVK SLPNSTLSEK LKVSWENPSP EKPSAPESAQ LSSSETPEAT
PRESKKPPAP PPKILSEKMK ACMSGVDASG SSQSSEAPET TSPEPTQVSV NGMDDGPESA
LQAMGIPGPA PEDAAASPAL PFSDLPSQFH PRSSSLGDLL RESPQHPRLP KEKLYRAQLE
VKVASKQTEK LLNQVLGSEP PPVCAESLLS QAVEQLRQAT QVLQEMRDLG ELNQETPGLV
QKRKELVTLY RRSAP
