PKP3_MOUSE
ID PKP3_MOUSE Reviewed; 797 AA.
AC Q9QY23; A4QPD8; Q0VGP3; Q3KQL7;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Plakophilin-3;
GN Name=Pkp3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=10381383; DOI=10.1242/jcs.112.14.2265;
RA Bonne S., van Hengel J., Nollet F., Kools P., van Roy F.;
RT "Plakophilin-3, a novel armadillo-like protein present in nuclei and
RT desmosomes of epithelial cells.";
RL J. Cell Sci. 112:2265-2276(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Colon, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123; SER-180 AND SER-331, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, Lung, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-261, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: May play a role in junctional plaques.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cell junction, desmosome. Note=Nuclear
CC and associated with desmosomes.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9QY23-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9QY23-2; Sequence=VSP_026139;
CC -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
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DR EMBL; AF136719; AAD55892.1; -; mRNA.
DR EMBL; BC090668; AAH90668.1; -; mRNA.
DR EMBL; BC106141; AAI06142.1; -; mRNA.
DR EMBL; BC139775; AAI39776.1; -; mRNA.
DR CCDS; CCDS21999.1; -. [Q9QY23-1]
DR CCDS; CCDS52437.1; -. [Q9QY23-2]
DR RefSeq; NP_001156396.1; NM_001162924.1. [Q9QY23-2]
DR RefSeq; NP_062736.2; NM_019762.2. [Q9QY23-1]
DR AlphaFoldDB; Q9QY23; -.
DR SMR; Q9QY23; -.
DR BioGRID; 207999; 6.
DR IntAct; Q9QY23; 2.
DR STRING; 10090.ENSMUSP00000101654; -.
DR iPTMnet; Q9QY23; -.
DR PhosphoSitePlus; Q9QY23; -.
DR MaxQB; Q9QY23; -.
DR PaxDb; Q9QY23; -.
DR PeptideAtlas; Q9QY23; -.
DR PRIDE; Q9QY23; -.
DR ProteomicsDB; 289754; -. [Q9QY23-1]
DR ProteomicsDB; 289755; -. [Q9QY23-2]
DR Antibodypedia; 4571; 294 antibodies from 33 providers.
DR DNASU; 56460; -.
DR Ensembl; ENSMUST00000066873; ENSMUSP00000069961; ENSMUSG00000054065. [Q9QY23-1]
DR Ensembl; ENSMUST00000106039; ENSMUSP00000101654; ENSMUSG00000054065. [Q9QY23-2]
DR GeneID; 56460; -.
DR KEGG; mmu:56460; -.
DR UCSC; uc009kjh.2; mouse. [Q9QY23-2]
DR UCSC; uc009kji.2; mouse. [Q9QY23-1]
DR CTD; 11187; -.
DR MGI; MGI:1891830; Pkp3.
DR VEuPathDB; HostDB:ENSMUSG00000054065; -.
DR eggNOG; KOG1048; Eukaryota.
DR GeneTree; ENSGT00940000159515; -.
DR HOGENOM; CLU_009111_2_0_1; -.
DR InParanoid; Q9QY23; -.
DR OMA; LDWPEAS; -.
DR OrthoDB; 233858at2759; -.
DR PhylomeDB; Q9QY23; -.
DR TreeFam; TF321877; -.
DR Reactome; R-MMU-6805567; Keratinization.
DR Reactome; R-MMU-6809371; Formation of the cornified envelope.
DR BioGRID-ORCS; 56460; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Pkp3; mouse.
DR PRO; PR:Q9QY23; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9QY23; protein.
DR Bgee; ENSMUSG00000054065; Expressed in lip and 173 other tissues.
DR ExpressionAtlas; Q9QY23; baseline and differential.
DR Genevisible; Q9QY23; MM.
DR GO; GO:0005912; C:adherens junction; IBA:GO_Central.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0001533; C:cornified envelope; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030057; C:desmosome; IDA:MGI.
DR GO; GO:1990124; C:messenger ribonucleoprotein complex; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005914; C:spot adherens junction; ISS:MGI.
DR GO; GO:0045294; F:alpha-catenin binding; ISO:MGI.
DR GO; GO:0045296; F:cadherin binding; IBA:GO_Central.
DR GO; GO:0050839; F:cell adhesion molecule binding; ISO:MGI.
DR GO; GO:0019899; F:enzyme binding; ISO:MGI.
DR GO; GO:0098609; P:cell-cell adhesion; IBA:GO_Central.
DR GO; GO:0007043; P:cell-cell junction assembly; IBA:GO_Central.
DR GO; GO:0002159; P:desmosome assembly; ISO:MGI.
DR GO; GO:1902373; P:negative regulation of mRNA catabolic process; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISO:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR028434; Plakophilin-3.
DR InterPro; IPR028435; Plakophilin/d_Catenin.
DR PANTHER; PTHR10372; PTHR10372; 1.
DR PANTHER; PTHR10372:SF1; PTHR10372:SF1; 1.
DR Pfam; PF00514; Arm; 2.
DR SMART; SM00185; ARM; 4.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell adhesion; Cell junction; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..797
FT /note="Plakophilin-3"
FT /id="PRO_0000064288"
FT REPEAT 305..348
FT /note="ARM 1"
FT REPEAT 351..390
FT /note="ARM 2"
FT REPEAT 393..432
FT /note="ARM 3"
FT REPEAT 449..487
FT /note="ARM 4"
FT REPEAT 491..536
FT /note="ARM 5"
FT REPEAT 596..637
FT /note="ARM 6"
FT REPEAT 645..684
FT /note="ARM 7"
FT REPEAT 689..730
FT /note="ARM 8"
FT REGION 58..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 219..241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 253..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 81
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y446"
FT MOD_RES 123
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 180
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 183
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y446"
FT MOD_RES 240
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y446"
FT MOD_RES 250
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y446"
FT MOD_RES 261
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 313
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y446"
FT MOD_RES 314
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y446"
FT MOD_RES 331
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..12
FT /note="MQEGNFLLSALQ -> MEPTAGSRTRMEPRRNCPTAGTSRMSQGASGGQTSG
FT K (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026139"
FT CONFLICT 182
FT /note="R -> P (in Ref. 1; AAD55892)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="E -> K (in Ref. 1; AAD55892)"
FT /evidence="ECO:0000305"
FT CONFLICT 459
FT /note="A -> P (in Ref. 1; AAD55892)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 797 AA; 87333 MW; C2ECF73BDE7323B1 CRC64;
MQEGNFLLSA LQPETGVCSL ALPSDLQLDR RGAEGPEADR LRAARVQEQV RARLLQLGQQ
SRHNGSAELD GSAESARGMP RGQYHTMQTG FSSRSQGMSG DKTSTFRPIA KPAYSPASWS
SRSAVDLTCS RRLSSAHNGG SAFGAVGYGG TQPTPPMPTR PVSFHERGGA ASRADYDTLS
LRSLRLGPGG LDDRYSVVSE QLEPAAASTY RAYAYERQAS SGSSRAGGLD WPEATEGPPS
RTIRAPAMRT LQRFQSSHRS RGGTGSVSGA GLEPVARAPS VRSLSLSLAD SGHLPDVRGL
DSYTGHRTLQ RLSSGFDDID LPSAVKYLMA SDPNLQVLGA AYIQHRCYSD AAAKKQARSL
QAVPRLVKLF NHANQEVQRH ATGAMRNLIY DNVDNKLALV EENGIFELLR TLREQDDELR
KNVTGILWNL SSSDHLKDRL ARDTLEQLTD LVLSPLSGAG GPPLIQQNAS EAEIFYNATG
FLRNLSSASQ ATRQKMRECH GLVDALVTYI NHALDVGKCE DKSVENAVCV LRNLSYRLYD
EMPPSALQRL EGRGRRDMAG APPGEMVGCF TPQSRRLREL PLTADALTFA EVSKDPKGLE
WLWSPQIVGL YNRLLQRCEL NRHTTEAAAG ALQNITAGDR RWAGVLSRLA LEQERILNPL
LDRVRTADHN QLRSLTGLIR NLSRNARNKD EMSTKVVSHL IEKLPGSVGE KCPPAEVLVN
IIAVLNNLVV ASPIAARDLL YFDGLRKLVF IKKKRDSPDS EKSSRAASSL LANLWQYSKL
HRDFRAKGYR KEDFLGP
