PKR2_MOUSE
ID PKR2_MOUSE Reviewed; 381 AA.
AC Q8K458; A2AMQ8; Q80XQ0; Q8BIR4;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Prokineticin receptor 2;
DE Short=PK-R2;
DE AltName: Full=G-protein coupled receptor 73-like 1;
GN Name=Prokr2; Synonyms=Gpr73l1, Pkr2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=12024206; DOI=10.1038/417405a;
RA Cheng M.Y., Bullock C.M., Li C., Lee A.G., Bermak J.C., Belluzzi J.,
RA Weaver D.R., Leslie F.M., Zhou Q.-Y.;
RT "Prokineticin 2 transmits the behavioural circadian rhythm of the
RT suprachiasmatic nucleus.";
RL Nature 417:405-410(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Medulla oblongata, Pituitary, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Receptor for prokineticin 2. Exclusively coupled to the G(q)
CC subclass of heteromeric G proteins. Activation leads to mobilization of
CC calcium, stimulation of phosphoinositide turnover and activation of
CC p44/p42 mitogen-activated protein kinase (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8NFJ6};
CC Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in several regions of the brain,
CC including paraventricular hypothalamic nucleus, dorsal medial
CC hypothalamic nucleus, paratenial thalamic nuclei, paracentral thalamic
CC nucleus, lateral habenular nucleus, lateral septal nucleus, lateral
CC globus pallidus and amygdala. Highest expression seen in
CC paraventricular thalamic nuclei and is also extensively expressed in
CC the suprachiasmatic nucleus.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC26971.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF487279; AAM49571.1; -; mRNA.
DR EMBL; AK030458; BAC26971.1; ALT_INIT; mRNA.
DR EMBL; AK041586; BAC30994.1; -; mRNA.
DR EMBL; AK080980; BAC38103.1; -; mRNA.
DR EMBL; AL807793; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC043116; AAH43116.1; -; mRNA.
DR CCDS; CCDS16773.1; -.
DR RefSeq; NP_659193.3; NM_144944.3.
DR RefSeq; XP_011237857.1; XM_011239555.2.
DR AlphaFoldDB; Q8K458; -.
DR SMR; Q8K458; -.
DR STRING; 10090.ENSMUSP00000056659; -.
DR GlyGen; Q8K458; 2 sites.
DR PhosphoSitePlus; Q8K458; -.
DR PaxDb; Q8K458; -.
DR PRIDE; Q8K458; -.
DR TopDownProteomics; Q8K458; -.
DR Antibodypedia; 8176; 245 antibodies from 32 providers.
DR DNASU; 246313; -.
DR Ensembl; ENSMUST00000049997; ENSMUSP00000056659; ENSMUSG00000050558.
DR Ensembl; ENSMUST00000110156; ENSMUSP00000105784; ENSMUSG00000050558.
DR GeneID; 246313; -.
DR KEGG; mmu:246313; -.
DR UCSC; uc008mmp.2; mouse.
DR CTD; 128674; -.
DR MGI; MGI:2181363; Prokr2.
DR VEuPathDB; HostDB:ENSMUSG00000050558; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000154544; -.
DR HOGENOM; CLU_009579_6_0_1; -.
DR InParanoid; Q8K458; -.
DR OMA; VDCIKLQ; -.
DR OrthoDB; 664455at2759; -.
DR PhylomeDB; Q8K458; -.
DR TreeFam; TF315303; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 246313; 0 hits in 71 CRISPR screens.
DR PRO; PR:Q8K458; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8K458; protein.
DR Bgee; ENSMUSG00000050558; Expressed in rostral migratory stream and 76 other tissues.
DR ExpressionAtlas; Q8K458; baseline and differential.
DR Genevisible; Q8K458; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISO:MGI.
DR GO; GO:0004983; F:neuropeptide Y receptor activity; IEA:InterPro.
DR GO; GO:0007623; P:circadian rhythm; IDA:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000611; NPY_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01012; NRPEPTIDEYR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..381
FT /note="Prokineticin receptor 2"
FT /id="PRO_0000070084"
FT TOPO_DOM 1..51
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..133
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..168
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..220
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..270
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..310
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 125..205
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 244
FT /note="S -> P (in Ref. 4; AAH43116)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="V -> L (in Ref. 2; BAC26971)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 381 AA; 43375 MW; 1981FD101324166D CRC64;
MGPQNRNTSF APDLNPPQDH VSLNYSYGDY DLPLGEDEDV TKTQTFFAAK IVIGVALAGI
MLVCGIGNFV FIAALARYKK LRNLTNLLIA NLAISDFLVA IVCCPFEMDY YVVRQLSWAH
GHVLCASVNY LRTVSLYVST NALLAIAIDR YLAIVHPLKP RMNYQTASFL IALVWMVSIL
IAVPSAYFTT ETILVIVKNQ EKIFCGQIWS VDQQLYYKSY FLFVFGLEFV GPVVTMTLCY
ARISQELWFK AVPGFQTEQI RKRLRCRRKT VLLLMGILTA YVLCWAPFYG FTIVRDFFPT
VVVKEKHYLT AFYVVECIAM SNSMINTICF VTVKNNTMKY FKKMLRLHWR PSHYGSKSSA
DLDLKTSGVP ATEEVDCIRL K
