PKRA_DROME
ID PKRA_DROME Reviewed; 422 AA.
AC Q9VNE2; A4V2F9;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Protein krasavietz;
DE AltName: Full=Eukaryotic translation initiation factor 5C domain-containing protein;
DE Short=Decp;
DE Short=eIF5C domain-containing protein;
DE AltName: Full=Protein extra bases;
GN Name=kra; Synonyms=ecp, eIF-5C, exba; ORFNames=CG2922;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Wan Y., Zhu K., Boulianne G.L., Xie W.;
RT "Drosophila eIF5C-containing protein gene (Decp).";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=12593794; DOI=10.1016/s0960-9822(03)00064-2;
RA Dubnau J., Chiang A.-S., Grady L., Barditch J., Gossweiler S., McNeil J.,
RA Smith P., Buldoc F., Scott R., Certa U., Broger C., Tully T.;
RT "The staufen/pumilio pathway is involved in Drosophila long-term memory.";
RL Curr. Biol. 13:286-296(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407; SER-412 AND SER-414, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: May be involved in memory formation.
CC {ECO:0000269|PubMed:12593794}.
CC -!- TISSUE SPECIFICITY: Expressed in mushroom bodies.
CC {ECO:0000269|PubMed:12593794}.
CC -!- DISRUPTION PHENOTYPE: Mutant flies show defective one-day memory but
CC normal learning. {ECO:0000269|PubMed:12593794}.
CC -!- SIMILARITY: Belongs to the BZW family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF325529; AAK01218.1; -; mRNA.
DR EMBL; AE014297; AAF51995.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF51996.1; -; Genomic_DNA.
DR EMBL; AE014297; AAG22214.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13246.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13247.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13248.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13249.1; -; Genomic_DNA.
DR EMBL; AY058505; AAL13734.1; -; mRNA.
DR RefSeq; NP_524238.1; NM_079514.3.
DR RefSeq; NP_730958.1; NM_169074.2.
DR RefSeq; NP_730959.1; NM_169075.2.
DR RefSeq; NP_730960.1; NM_169076.2.
DR RefSeq; NP_730961.1; NM_169077.2.
DR RefSeq; NP_730962.1; NM_169078.2.
DR RefSeq; NP_730963.1; NM_169079.2.
DR AlphaFoldDB; Q9VNE2; -.
DR SMR; Q9VNE2; -.
DR BioGRID; 65886; 15.
DR IntAct; Q9VNE2; 3.
DR STRING; 7227.FBpp0078393; -.
DR iPTMnet; Q9VNE2; -.
DR PaxDb; Q9VNE2; -.
DR PRIDE; Q9VNE2; -.
DR DNASU; 40680; -.
DR EnsemblMetazoa; FBtr0078744; FBpp0078393; FBgn0250753.
DR EnsemblMetazoa; FBtr0078745; FBpp0078394; FBgn0250753.
DR EnsemblMetazoa; FBtr0078746; FBpp0078395; FBgn0250753.
DR EnsemblMetazoa; FBtr0078747; FBpp0078396; FBgn0250753.
DR EnsemblMetazoa; FBtr0078748; FBpp0078397; FBgn0250753.
DR EnsemblMetazoa; FBtr0078749; FBpp0078398; FBgn0250753.
DR EnsemblMetazoa; FBtr0078750; FBpp0078399; FBgn0250753.
DR GeneID; 40680; -.
DR KEGG; dme:Dmel_CG2922; -.
DR UCSC; CG2922-RB; d. melanogaster.
DR CTD; 40680; -.
DR FlyBase; FBgn0250753; kra.
DR VEuPathDB; VectorBase:FBgn0250753; -.
DR eggNOG; KOG2297; Eukaryota.
DR GeneTree; ENSGT00390000012561; -.
DR HOGENOM; CLU_032849_0_1_1; -.
DR InParanoid; Q9VNE2; -.
DR OMA; ELIQCIW; -.
DR OrthoDB; 653740at2759; -.
DR PhylomeDB; Q9VNE2; -.
DR BioGRID-ORCS; 40680; 2 hits in 3 CRISPR screens.
DR ChiTaRS; kra; fly.
DR GenomeRNAi; 40680; -.
DR PRO; PR:Q9VNE2; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0250753; Expressed in ovary and 10 other tissues.
DR Genevisible; Q9VNE2; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0044295; C:axonal growth cone; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0030175; C:filopodium; IDA:FlyBase.
DR GO; GO:0043025; C:neuronal cell body; IDA:FlyBase.
DR GO; GO:0043024; F:ribosomal small subunit binding; IDA:FlyBase.
DR GO; GO:0043022; F:ribosome binding; IDA:FlyBase.
DR GO; GO:0031369; F:translation initiation factor binding; IPI:FlyBase.
DR GO; GO:0016199; P:axon midline choice point recognition; IMP:FlyBase.
DR GO; GO:0048149; P:behavioral response to ethanol; IMP:FlyBase.
DR GO; GO:0007616; P:long-term memory; IMP:UniProtKB.
DR GO; GO:0017148; P:negative regulation of translation; IDA:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:FlyBase.
DR GO; GO:0051491; P:positive regulation of filopodium assembly; IMP:FlyBase.
DR CDD; cd11560; W2_eIF5C_like; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR043510; W2_BZW1/2.
DR InterPro; IPR003307; W2_domain.
DR Pfam; PF02020; W2; 1.
DR SMART; SM00515; eIF5C; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51363; W2; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..422
FT /note="Protein krasavietz"
FT /id="PRO_0000254625"
FT DOMAIN 244..415
FT /note="W2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00695"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 407
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 412
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 414
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 422 AA; 49225 MW; E99F77F94A41E839 CRC64;
MSQKTERPVL SGQRIKTRKR DEREKYDPTG FRDAVIAGLE KTEGDLEQIS KYLDSAGNKL
DYRRYGEVLF DILIAGGLLV PGGSISQDGE KPRTSYCIFD APESMESMRN HEQVFVKLIR
RYKYLEKMFE EEMGKVLLFV KGFTPSERIK LARMTALWLV NGSVPPNVLL VLNNEHLIKD
GIALEFLLEL FQTFKQEKGI AYLIQALKKG GLESKLMDFF PPNKRTEEYF KQVFLDKELN
EIVKLHKAQA SQEAKRELQQ ALIDDINDEK PYNEITSDIK DFSQRTNIPD HEIIVIIWST
IMSLGEWNKK EELVTDQAVR HLKNYCPLLQ AFASTDRSEL ALILKVQEFC YENMNFMKAF
QKIILLFYKT EVLSEEIILR WYKEGHSNKG KMHFLEQMRK FVEWLQSAEE ESESEDEQKN
GE
